Down-regulation of catalase activity contributes to senescence induction in wheat leaves exposed to shading stress
In shaded wheat (Triticum aestivum L.) leaves, the suppression of blue radiation (BR) triggers senescence. This phenomenon is correlated to an increase in oxidative stress symptoms and a decrease of catalase (CAT) activity, among other traits. Previous data suggest that the radiation signal transduc...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Springer
2018
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| Online Access: | http://hdl.handle.net/20.500.12123/3558 https://link.springer.com/article/10.1007/s10535-014-0480-z https://doi.org/10.1007/s10535-014-0480-z |
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| author | Causin, Humberto Fabio Marchetti, Cintia Florencia Pena, Liliana Beatriz Gallego, Susana Mabel Barneix, Atilio Jose |
| author_browse | Barneix, Atilio Jose Causin, Humberto Fabio Gallego, Susana Mabel Marchetti, Cintia Florencia Pena, Liliana Beatriz |
| author_facet | Causin, Humberto Fabio Marchetti, Cintia Florencia Pena, Liliana Beatriz Gallego, Susana Mabel Barneix, Atilio Jose |
| author_sort | Causin, Humberto Fabio |
| collection | INTA Digital |
| description | In shaded wheat (Triticum aestivum L.) leaves, the suppression of blue radiation (BR) triggers senescence. This phenomenon is correlated to an increase in oxidative stress symptoms and a decrease of catalase (CAT) activity, among other traits. Previous data suggest that the radiation signal transduction pathway may involve changes in Ca2+ and H2O2 homeostasis. For better a understanding of the interaction among the spectral composition of radiation, Ca2+ availability, and the antioxidant metabolism in the regulation of shade-induced senescence, detached wheat leaves were placed in a growth chamber and exposed to either blue (B, high BR transmittance) and/or green (G, very low BR transmittance) Lee® filters in the absence or presence of 0.8 mM verapamil (a Ca2+ channels blocker), 4.0 mM EGTA (a Ca2+chelator), or 8.0 mM 3-amino-1,2,4-triazole (a CAT inhibitor). At defined time points, the leaf samples were analyzed for changes in chlorophyll content, specific activities of CAT, ascorbate peroxidase (APX), and guaiacol peroxidase (POX), CAT isozymes, and gene expression of CAT1, CAT2, and two senescence markers (TaSAG1 and TaSAG3). BR transmittance decreased the chlorophyll degradation rate and SAG genes expression either in leaves continuously exposed under the B filter, as well as in leaves previously exposed under the G filter. The effect of BR was associated with the maintenance of a high CAT (but not APX and POX) activity, and it was suppressed either in the presence of 3-AT or when Ca2+ availability was decreased. BR altered the CAT activity both at the transcriptional and at the posttranscriptional level. Nevertheless, different responses of CAT isozymes and CAT genes expression profiles to specific treatment combinations indicate that they differed in their regulatory pathways. |
| format | info:ar-repo/semantics/artículo |
| id | INTA3558 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA35582019-01-04T18:34:47Z Down-regulation of catalase activity contributes to senescence induction in wheat leaves exposed to shading stress Causin, Humberto Fabio Marchetti, Cintia Florencia Pena, Liliana Beatriz Gallego, Susana Mabel Barneix, Atilio Jose Trigo Triticum Aestivum Catalasa Antioxidantes Calcio Radiación Estrés Oxidativo Expresión Génica Wheat Catalase Antioxidants Calcium Radiation Oxidative Stress Gene Expression Blue Radiation Senescencia In shaded wheat (Triticum aestivum L.) leaves, the suppression of blue radiation (BR) triggers senescence. This phenomenon is correlated to an increase in oxidative stress symptoms and a decrease of catalase (CAT) activity, among other traits. Previous data suggest that the radiation signal transduction pathway may involve changes in Ca2+ and H2O2 homeostasis. For better a understanding of the interaction among the spectral composition of radiation, Ca2+ availability, and the antioxidant metabolism in the regulation of shade-induced senescence, detached wheat leaves were placed in a growth chamber and exposed to either blue (B, high BR transmittance) and/or green (G, very low BR transmittance) Lee® filters in the absence or presence of 0.8 mM verapamil (a Ca2+ channels blocker), 4.0 mM EGTA (a Ca2+chelator), or 8.0 mM 3-amino-1,2,4-triazole (a CAT inhibitor). At defined time points, the leaf samples were analyzed for changes in chlorophyll content, specific activities of CAT, ascorbate peroxidase (APX), and guaiacol peroxidase (POX), CAT isozymes, and gene expression of CAT1, CAT2, and two senescence markers (TaSAG1 and TaSAG3). BR transmittance decreased the chlorophyll degradation rate and SAG genes expression either in leaves continuously exposed under the B filter, as well as in leaves previously exposed under the G filter. The effect of BR was associated with the maintenance of a high CAT (but not APX and POX) activity, and it was suppressed either in the presence of 3-AT or when Ca2+ availability was decreased. BR altered the CAT activity both at the transcriptional and at the posttranscriptional level. Nevertheless, different responses of CAT isozymes and CAT genes expression profiles to specific treatment combinations indicate that they differed in their regulatory pathways. Instituto de Suelos Fil: Causin, Humberto Fabio. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Marchetti, Cintia Florencia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Pena, Liliana Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Gallego, Susana Mabel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Barneix, Atilio José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Suelos; Argentina 2018-10-04T16:13:50Z 2018-10-04T16:13:50Z 2015-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/3558 https://link.springer.com/article/10.1007/s10535-014-0480-z 0006-3134 1573-8264 (Online) https://doi.org/10.1007/s10535-014-0480-z eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Biologia plantarum 59 (1) : 154–162. (January 2015) |
| spellingShingle | Trigo Triticum Aestivum Catalasa Antioxidantes Calcio Radiación Estrés Oxidativo Expresión Génica Wheat Catalase Antioxidants Calcium Radiation Oxidative Stress Gene Expression Blue Radiation Senescencia Causin, Humberto Fabio Marchetti, Cintia Florencia Pena, Liliana Beatriz Gallego, Susana Mabel Barneix, Atilio Jose Down-regulation of catalase activity contributes to senescence induction in wheat leaves exposed to shading stress |
| title | Down-regulation of catalase activity contributes to senescence induction in wheat leaves exposed to shading stress |
| title_full | Down-regulation of catalase activity contributes to senescence induction in wheat leaves exposed to shading stress |
| title_fullStr | Down-regulation of catalase activity contributes to senescence induction in wheat leaves exposed to shading stress |
| title_full_unstemmed | Down-regulation of catalase activity contributes to senescence induction in wheat leaves exposed to shading stress |
| title_short | Down-regulation of catalase activity contributes to senescence induction in wheat leaves exposed to shading stress |
| title_sort | down regulation of catalase activity contributes to senescence induction in wheat leaves exposed to shading stress |
| topic | Trigo Triticum Aestivum Catalasa Antioxidantes Calcio Radiación Estrés Oxidativo Expresión Génica Wheat Catalase Antioxidants Calcium Radiation Oxidative Stress Gene Expression Blue Radiation Senescencia |
| url | http://hdl.handle.net/20.500.12123/3558 https://link.springer.com/article/10.1007/s10535-014-0480-z https://doi.org/10.1007/s10535-014-0480-z |
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