Antagonistic effects of Bacillus subtilis subsp. subtilis and B.amyloliquefaciens against Macrophomina phaseolina: SEM study offungal changes and UV-MALDI-TOF MS analysis of their bioactivecompounds
The antifungal effect of Bacillus subtilis subsp. subtilis PGPMori7 and Bacillus amyloliquefaciens PGPBacCA1 was evaluated against Macrophomina phaseolina (Tassi) Goid. Cell suspension (CS), cell-free supernatant (CFS) and the lipopeptide fraction (LF) of PGPMori7 and PGPBacCA1 were screened against...
| Autores principales: | , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Elsevier
2018
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/3540 https://www.sciencedirect.com/science/article/pii/S0944501315300094?via%3Dihub https://doi.org/10.1016/j.micres.2015.09.005 |
| _version_ | 1855035160294064128 |
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| author | Torres, María Julia Perez Brandan, Carolina Petroselli, Gabriela Erra Balsells, Rosa Audisio, Marcela Carina |
| author_browse | Audisio, Marcela Carina Erra Balsells, Rosa Perez Brandan, Carolina Petroselli, Gabriela Torres, María Julia |
| author_facet | Torres, María Julia Perez Brandan, Carolina Petroselli, Gabriela Erra Balsells, Rosa Audisio, Marcela Carina |
| author_sort | Torres, María Julia |
| collection | INTA Digital |
| description | The antifungal effect of Bacillus subtilis subsp. subtilis PGPMori7 and Bacillus amyloliquefaciens PGPBacCA1 was evaluated against Macrophomina phaseolina (Tassi) Goid. Cell suspension (CS), cell-free supernatant (CFS) and the lipopeptide fraction (LF) of PGPMori7 and PGPBacCA1 were screened against three different M. phaseolina strains. CS exhibited the highest inhibitory effect (around 50%) when compared to those of CFS and LF, regardless of the fungal strain studied. The synthesis of lipopeptides was studied by UV-MALDI TOF. Chemical analysis of Bacillus metabolite synthesis revealed that surfactin and iturin were mainly produced in liquid medium. Potential fengycin was also co-produced when both Bacillus were cultivated in solid medium. In co-culture assays, the bacterial colony-fungal mycelium interface at the inhibition zone was evaluated by both scanning electron microscopy (SEM) and UV-MALDI TOF, the former to determine the structural changes on M. phaseolina cells and the latter to identify the main bioactive molecules involved in the inhibitory effect. PGPBacCA1 produced surfactin, iturin and fengycin in the inhibition zone while PGPMori7 only produced these metabolites within its colony and not in the narrow inhibition zone. Interestingly, SEM revealed that PGPBacCA1 induced damage in M. phaseolina sclerotia, generating a fungicidal effect as no growth was observed when normal growth conditions were reestablished. In turn, PGPMori7 inhibited the growth of the Macrophomina mycelium without fungal injury, resulting only in a fungistatic activity. From these results, it was determined that the two bacilli significantly inhibited the growth of an important phytopathogenic fungus by at least two different mechanisms: lipopeptide synthesis and competition among |
| format | info:ar-repo/semantics/artículo |
| id | INTA3540 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA35402018-10-02T17:07:41Z Antagonistic effects of Bacillus subtilis subsp. subtilis and B.amyloliquefaciens against Macrophomina phaseolina: SEM study offungal changes and UV-MALDI-TOF MS analysis of their bioactivecompounds Torres, María Julia Perez Brandan, Carolina Petroselli, Gabriela Erra Balsells, Rosa Audisio, Marcela Carina Bacillus Subtilis Macrophomina Phaseolina Microscopia de Barrido Scanning Microscopy Bacillus Amyloliquefaciens Lipopeptides Scanning Electron Microscopy SEM UV-MALDI TOF MS The antifungal effect of Bacillus subtilis subsp. subtilis PGPMori7 and Bacillus amyloliquefaciens PGPBacCA1 was evaluated against Macrophomina phaseolina (Tassi) Goid. Cell suspension (CS), cell-free supernatant (CFS) and the lipopeptide fraction (LF) of PGPMori7 and PGPBacCA1 were screened against three different M. phaseolina strains. CS exhibited the highest inhibitory effect (around 50%) when compared to those of CFS and LF, regardless of the fungal strain studied. The synthesis of lipopeptides was studied by UV-MALDI TOF. Chemical analysis of Bacillus metabolite synthesis revealed that surfactin and iturin were mainly produced in liquid medium. Potential fengycin was also co-produced when both Bacillus were cultivated in solid medium. In co-culture assays, the bacterial colony-fungal mycelium interface at the inhibition zone was evaluated by both scanning electron microscopy (SEM) and UV-MALDI TOF, the former to determine the structural changes on M. phaseolina cells and the latter to identify the main bioactive molecules involved in the inhibitory effect. PGPBacCA1 produced surfactin, iturin and fengycin in the inhibition zone while PGPMori7 only produced these metabolites within its colony and not in the narrow inhibition zone. Interestingly, SEM revealed that PGPBacCA1 induced damage in M. phaseolina sclerotia, generating a fungicidal effect as no growth was observed when normal growth conditions were reestablished. In turn, PGPMori7 inhibited the growth of the Macrophomina mycelium without fungal injury, resulting only in a fungistatic activity. From these results, it was determined that the two bacilli significantly inhibited the growth of an important phytopathogenic fungus by at least two different mechanisms: lipopeptide synthesis and competition among EEA Salta Fil: Torres, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina Fil: Perez Brandan, Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Petroselli, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina Fil: Erra Balsells, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina Fil: Audisio, Marcela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina 2018-10-02T17:01:37Z 2018-10-02T17:01:37Z 2016-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/3540 https://www.sciencedirect.com/science/article/pii/S0944501315300094?via%3Dihub 0944-5013 https://doi.org/10.1016/j.micres.2015.09.005 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Microbiological research 182 : 31-39. (January 2016) |
| spellingShingle | Bacillus Subtilis Macrophomina Phaseolina Microscopia de Barrido Scanning Microscopy Bacillus Amyloliquefaciens Lipopeptides Scanning Electron Microscopy SEM UV-MALDI TOF MS Torres, María Julia Perez Brandan, Carolina Petroselli, Gabriela Erra Balsells, Rosa Audisio, Marcela Carina Antagonistic effects of Bacillus subtilis subsp. subtilis and B.amyloliquefaciens against Macrophomina phaseolina: SEM study offungal changes and UV-MALDI-TOF MS analysis of their bioactivecompounds |
| title | Antagonistic effects of Bacillus subtilis subsp. subtilis and B.amyloliquefaciens against Macrophomina phaseolina: SEM study offungal changes and UV-MALDI-TOF MS analysis of their bioactivecompounds |
| title_full | Antagonistic effects of Bacillus subtilis subsp. subtilis and B.amyloliquefaciens against Macrophomina phaseolina: SEM study offungal changes and UV-MALDI-TOF MS analysis of their bioactivecompounds |
| title_fullStr | Antagonistic effects of Bacillus subtilis subsp. subtilis and B.amyloliquefaciens against Macrophomina phaseolina: SEM study offungal changes and UV-MALDI-TOF MS analysis of their bioactivecompounds |
| title_full_unstemmed | Antagonistic effects of Bacillus subtilis subsp. subtilis and B.amyloliquefaciens against Macrophomina phaseolina: SEM study offungal changes and UV-MALDI-TOF MS analysis of their bioactivecompounds |
| title_short | Antagonistic effects of Bacillus subtilis subsp. subtilis and B.amyloliquefaciens against Macrophomina phaseolina: SEM study offungal changes and UV-MALDI-TOF MS analysis of their bioactivecompounds |
| title_sort | antagonistic effects of bacillus subtilis subsp subtilis and b amyloliquefaciens against macrophomina phaseolina sem study offungal changes and uv maldi tof ms analysis of their bioactivecompounds |
| topic | Bacillus Subtilis Macrophomina Phaseolina Microscopia de Barrido Scanning Microscopy Bacillus Amyloliquefaciens Lipopeptides Scanning Electron Microscopy SEM UV-MALDI TOF MS |
| url | http://hdl.handle.net/20.500.12123/3540 https://www.sciencedirect.com/science/article/pii/S0944501315300094?via%3Dihub https://doi.org/10.1016/j.micres.2015.09.005 |
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