Screening of pesticide residues in honeybee wax comb by LC-ESI-MS/MS. A pilot study

A developed multi-residue method using microflow-LC-ESI-QqQ-MS provided a wide-scope analysis for medium-polar and polar pesticide residues (120 compounds including breakdown products). Honeybee wax comb samples were extracted using a generic QuEChERS based procedure. Acceptable recoveries at concen...

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Autores principales: Herrera López, Sonia, Lozano, Ana, Sosa, Alexis Lionel, Hernando, M. Dolores, Rodríguez Fernández-Alba, Amadeo
Formato: info:ar-repo/semantics/artículo
Lenguaje:Inglés
Publicado: 2018
Materias:
Acceso en línea:https://www.sciencedirect.com/science/article/pii/S004565351630892X?via%3Dihub
http://hdl.handle.net/20.500.12123/3521
https://doi.org/10.1016/j.chemosphere.2016.07.008
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author Herrera López, Sonia
Lozano, Ana
Sosa, Alexis Lionel
Hernando, M. Dolores
Rodríguez Fernández-Alba, Amadeo
author_browse Hernando, M. Dolores
Herrera López, Sonia
Lozano, Ana
Rodríguez Fernández-Alba, Amadeo
Sosa, Alexis Lionel
author_facet Herrera López, Sonia
Lozano, Ana
Sosa, Alexis Lionel
Hernando, M. Dolores
Rodríguez Fernández-Alba, Amadeo
author_sort Herrera López, Sonia
collection INTA Digital
description A developed multi-residue method using microflow-LC-ESI-QqQ-MS provided a wide-scope analysis for medium-polar and polar pesticide residues (120 compounds including breakdown products). Honeybee wax comb samples were extracted using a generic QuEChERS based procedure. Acceptable recoveries at concentration levels of 5 and 50 μg kg−1 were within the 70–120% range with an associated precision RSD <20%. The LOQ values were mostly 5 μg kg−1 for almost all pesticides. Aprox. 31 of 120 LC-amenable pesticides tested (25.8%) were detected in a pilot study of 60 samples. Pesticide residues detected using the proposed method were: the breakdown products of amitraz, DMPF and DMF, an acaricide used for Varroa mite control, with a range of concentration from 5 to 464 μg kg−1 (sum of DMPF + DMF), organophosphate insecticides from 1 to 464 μg kg−1, acaricides at concentrations > 9 μg kg−1; fungicides at concentrations ranging from 1 to 23 μg kg−1. The number of positive detections due to herbicides was lower as expected and at a lower level of concentration, from 1 to 5.9 μg kg−1.
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institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
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spelling INTA35212018-10-01T13:31:18Z Screening of pesticide residues in honeybee wax comb by LC-ESI-MS/MS. A pilot study Herrera López, Sonia Lozano, Ana Sosa, Alexis Lionel Hernando, M. Dolores Rodríguez Fernández-Alba, Amadeo Abeja Melífera Cera de Abeja Productos de la Colmena Residuos de Plaguicidas Técnicas de Trazadores Honey Bees Beeswax Hive Products Pesticide Residues Tracer Techniques LC-ESI-MS/MS A developed multi-residue method using microflow-LC-ESI-QqQ-MS provided a wide-scope analysis for medium-polar and polar pesticide residues (120 compounds including breakdown products). Honeybee wax comb samples were extracted using a generic QuEChERS based procedure. Acceptable recoveries at concentration levels of 5 and 50 μg kg−1 were within the 70–120% range with an associated precision RSD <20%. The LOQ values were mostly 5 μg kg−1 for almost all pesticides. Aprox. 31 of 120 LC-amenable pesticides tested (25.8%) were detected in a pilot study of 60 samples. Pesticide residues detected using the proposed method were: the breakdown products of amitraz, DMPF and DMF, an acaricide used for Varroa mite control, with a range of concentration from 5 to 464 μg kg−1 (sum of DMPF + DMF), organophosphate insecticides from 1 to 464 μg kg−1, acaricides at concentrations > 9 μg kg−1; fungicides at concentrations ranging from 1 to 23 μg kg−1. The number of positive detections due to herbicides was lower as expected and at a lower level of concentration, from 1 to 5.9 μg kg−1. EEA Concordia Fil: Herrera López, Sonia. Universidad de Almería. Departamento de Química y Física. Campus de Excelencia Internacional en Agroalimentación; España. European Union Reference Laboratory for Pesticide Residues in Fruit and Vegetables; España Fil: Lozano, Ana. Universidad de Almería. Departamento de Química y Física. Campus de Excelencia Internacional en Agroalimentación; España. European Union Reference Laboratory for Pesticide Residues in Fruit and Vegetables; España Fil: Sosa, Alexis Lionel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Hernando, M. Dolores. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA); España Fil: Rodríguez Fernández-Alba, Amadeo. Universidad de Almería. Departamento de Química y Física. Campus de Excelencia Internacional en Agroalimentación; España. European Union Reference Laboratory for Pesticide Residues in Fruit and Vegetables; España 2018-10-01T13:26:00Z 2018-10-01T13:26:00Z 2016-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S004565351630892X?via%3Dihub http://hdl.handle.net/20.500.12123/3521 0045-6535 1879-1298 https://doi.org/10.1016/j.chemosphere.2016.07.008 eng info:eu-repo/semantics/restrictedAccess application/pdf Chemosphere 163 : 44-53 (November 2016)
spellingShingle Abeja Melífera
Cera de Abeja
Productos de la Colmena
Residuos de Plaguicidas
Técnicas de Trazadores
Honey Bees
Beeswax
Hive Products
Pesticide Residues
Tracer Techniques
LC-ESI-MS/MS
Herrera López, Sonia
Lozano, Ana
Sosa, Alexis Lionel
Hernando, M. Dolores
Rodríguez Fernández-Alba, Amadeo
Screening of pesticide residues in honeybee wax comb by LC-ESI-MS/MS. A pilot study
title Screening of pesticide residues in honeybee wax comb by LC-ESI-MS/MS. A pilot study
title_full Screening of pesticide residues in honeybee wax comb by LC-ESI-MS/MS. A pilot study
title_fullStr Screening of pesticide residues in honeybee wax comb by LC-ESI-MS/MS. A pilot study
title_full_unstemmed Screening of pesticide residues in honeybee wax comb by LC-ESI-MS/MS. A pilot study
title_short Screening of pesticide residues in honeybee wax comb by LC-ESI-MS/MS. A pilot study
title_sort screening of pesticide residues in honeybee wax comb by lc esi ms ms a pilot study
topic Abeja Melífera
Cera de Abeja
Productos de la Colmena
Residuos de Plaguicidas
Técnicas de Trazadores
Honey Bees
Beeswax
Hive Products
Pesticide Residues
Tracer Techniques
LC-ESI-MS/MS
url https://www.sciencedirect.com/science/article/pii/S004565351630892X?via%3Dihub
http://hdl.handle.net/20.500.12123/3521
https://doi.org/10.1016/j.chemosphere.2016.07.008
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