Genetics and mapping of a new anthracnose resistance locus in Andean common bean Paloma
Background: The Andean cultivar Paloma is resistant to Mesoamerican and Andean races of Colletotrichum lindemuthianum, the fungal pathogen that causes the destructive anthracnose disease in common bean. Remarkably, Paloma is resistant to Mesoamerican races 2047 and 3481, which are among the most vir...
| Autores principales: | , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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2018
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| Acceso en línea: | https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-017-3685-7 http://hdl.handle.net/20.500.12123/3488 https://doi.org/10.1186/s12864-017-3685-7 |
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| author | Castro, Sandra Aparecida de Lima Gonçalves-Vidigal, Maria Celeste Gilio, Thiago Alexandre Santana Lacanallo, Giselly Figueiredo Valentini, Giseli Martins, Vanusa da Silva Ramos Qijian, Song Galvan, Marta Zulema Hurtado-Gonzales, Oscar P. Pastor-Corrales, Marcial Antonio |
| author_browse | Castro, Sandra Aparecida de Lima Galvan, Marta Zulema Gilio, Thiago Alexandre Santana Gonçalves-Vidigal, Maria Celeste Hurtado-Gonzales, Oscar P. Lacanallo, Giselly Figueiredo Martins, Vanusa da Silva Ramos Pastor-Corrales, Marcial Antonio Qijian, Song Valentini, Giseli |
| author_facet | Castro, Sandra Aparecida de Lima Gonçalves-Vidigal, Maria Celeste Gilio, Thiago Alexandre Santana Lacanallo, Giselly Figueiredo Valentini, Giseli Martins, Vanusa da Silva Ramos Qijian, Song Galvan, Marta Zulema Hurtado-Gonzales, Oscar P. Pastor-Corrales, Marcial Antonio |
| author_sort | Castro, Sandra Aparecida de Lima |
| collection | INTA Digital |
| description | Background: The Andean cultivar Paloma is resistant to Mesoamerican and Andean races of Colletotrichum lindemuthianum, the fungal pathogen that causes the destructive anthracnose disease in common bean. Remarkably, Paloma is resistant to Mesoamerican races 2047 and 3481, which are among the most virulent races of the anthracnose pathogen. Most genes conferring anthracnose resistance in common bean are overcome by these races. The genetic mapping and the relationship between the resistant Co-Pa gene of Paloma and previously characterized anthracnose resistance genes can be a great contribution for breeding programs.
Results: The inheritance of resistance studies for Paloma was performed in F2 population from the cross Paloma (resistant) × Cornell 49–242 (susceptible) inoculated with race 2047, and in F2 and F2:3 generations from the cross Paloma (resistant) × PI 207262 (susceptible) inoculated with race 3481. The results of these studies demonstrated that a single dominant gene confers the resistance in Paloma. Allelism tests performed with multiple races of C. lindemuthianum showed that the resistance gene in Paloma, provisionally named Co-Pa, is independent from the anthracnose resistance genes Co-1, Co-2, Co-3, Co-4, Co-5, Co-6, Co-12, Co-13, Co-14, Co-15 and Co-16. Bulk segregant analysis using the SNP chip BARCBean6K_3 positioned the approximate location of Co-Pa in the lower arm of chromosome Pv01. Further mapping analysis located the Co-Pa gene at a 390 kb region of Pv01 flanked by SNP markers SS82 and SS83 at a distance of 1.3 and 2.1 cM, respectively.
Conclusions: The results presented here showed that Paloma cultivar has a new dominant gene conferring resistance to anthracnose, which is independent from those genes previously described. The linkage between the Co-Pa gene and the SS82 and SS83 SNP markers will be extremely important for marker-assisted introgression of the gene into elite cultivars in order to enhance resistance. |
| format | info:ar-repo/semantics/artículo |
| id | INTA3488 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA34882018-09-27T12:29:06Z Genetics and mapping of a new anthracnose resistance locus in Andean common bean Paloma Castro, Sandra Aparecida de Lima Gonçalves-Vidigal, Maria Celeste Gilio, Thiago Alexandre Santana Lacanallo, Giselly Figueiredo Valentini, Giseli Martins, Vanusa da Silva Ramos Qijian, Song Galvan, Marta Zulema Hurtado-Gonzales, Oscar P. Pastor-Corrales, Marcial Antonio Fríjol (phaseolus) Variedades Phaseolus vulgaris Genética Resistencia a la Enfermedad Antracnosis Kidney Beans Varieties Genetics Disease Resistance Anthracnosis Poroto Variedad Paloma Background: The Andean cultivar Paloma is resistant to Mesoamerican and Andean races of Colletotrichum lindemuthianum, the fungal pathogen that causes the destructive anthracnose disease in common bean. Remarkably, Paloma is resistant to Mesoamerican races 2047 and 3481, which are among the most virulent races of the anthracnose pathogen. Most genes conferring anthracnose resistance in common bean are overcome by these races. The genetic mapping and the relationship between the resistant Co-Pa gene of Paloma and previously characterized anthracnose resistance genes can be a great contribution for breeding programs. Results: The inheritance of resistance studies for Paloma was performed in F2 population from the cross Paloma (resistant) × Cornell 49–242 (susceptible) inoculated with race 2047, and in F2 and F2:3 generations from the cross Paloma (resistant) × PI 207262 (susceptible) inoculated with race 3481. The results of these studies demonstrated that a single dominant gene confers the resistance in Paloma. Allelism tests performed with multiple races of C. lindemuthianum showed that the resistance gene in Paloma, provisionally named Co-Pa, is independent from the anthracnose resistance genes Co-1, Co-2, Co-3, Co-4, Co-5, Co-6, Co-12, Co-13, Co-14, Co-15 and Co-16. Bulk segregant analysis using the SNP chip BARCBean6K_3 positioned the approximate location of Co-Pa in the lower arm of chromosome Pv01. Further mapping analysis located the Co-Pa gene at a 390 kb region of Pv01 flanked by SNP markers SS82 and SS83 at a distance of 1.3 and 2.1 cM, respectively. Conclusions: The results presented here showed that Paloma cultivar has a new dominant gene conferring resistance to anthracnose, which is independent from those genes previously described. The linkage between the Co-Pa gene and the SS82 and SS83 SNP markers will be extremely important for marker-assisted introgression of the gene into elite cultivars in order to enhance resistance. EEA Salta Fil: Castro, Sandra Aparecida de Lima. Universidade Estadual de Maringá. Departamento de Agronomia; Brasil Fil: Gonçalves-Vidigal, Maria Celeste. Universidade Estadual de Maringá. Departamento de Agronomia; Brasil Fil: Gilio, Thiago Alexandre Santana. Universidade Estadual de Maringá. Departamento de Agronomia; Brasil Fil: Lacanallo, Giselly Figueiredo. Universidade Estadual de Maringá. Departamento de Agronomia; Brasil Fil: Valentini, Giseli. Universidade Estadual de Maringá. Departamento de Agronomia; Brasil Fil: Martins, Vanusa da Silva Ramos. Universidade Estadual de Maringá. Departamento de Agronomia; Brasil Fil: Qijian, Song. United States Department of Agriculture. Agricultural Research Service. Soybean Genomics and Improvement Laboratory; Estados Unidos Fil: Galvan, Marta Zulema. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Hurtado-Gonzales, Oscar P. United States Department of Agriculture. Agricultural Research Service. Soybean Genomics and Improvement Laboratory; Estados Unidos Fil: Pastor-Corrales, Marcial Antonio. United States Department of Agriculture. Agricultural Research Service. Soybean Genomics and Improvement Laboratory; Estados Unidos 2018-09-27T12:27:20Z 2018-09-27T12:27:20Z 2017-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-017-3685-7 http://hdl.handle.net/20.500.12123/3488 1471-2164 https://doi.org/10.1186/s12864-017-3685-7 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf BMC Genomics 18 : 306 (April 2017) |
| spellingShingle | Fríjol (phaseolus) Variedades Phaseolus vulgaris Genética Resistencia a la Enfermedad Antracnosis Kidney Beans Varieties Genetics Disease Resistance Anthracnosis Poroto Variedad Paloma Castro, Sandra Aparecida de Lima Gonçalves-Vidigal, Maria Celeste Gilio, Thiago Alexandre Santana Lacanallo, Giselly Figueiredo Valentini, Giseli Martins, Vanusa da Silva Ramos Qijian, Song Galvan, Marta Zulema Hurtado-Gonzales, Oscar P. Pastor-Corrales, Marcial Antonio Genetics and mapping of a new anthracnose resistance locus in Andean common bean Paloma |
| title | Genetics and mapping of a new anthracnose resistance locus in Andean common bean Paloma |
| title_full | Genetics and mapping of a new anthracnose resistance locus in Andean common bean Paloma |
| title_fullStr | Genetics and mapping of a new anthracnose resistance locus in Andean common bean Paloma |
| title_full_unstemmed | Genetics and mapping of a new anthracnose resistance locus in Andean common bean Paloma |
| title_short | Genetics and mapping of a new anthracnose resistance locus in Andean common bean Paloma |
| title_sort | genetics and mapping of a new anthracnose resistance locus in andean common bean paloma |
| topic | Fríjol (phaseolus) Variedades Phaseolus vulgaris Genética Resistencia a la Enfermedad Antracnosis Kidney Beans Varieties Genetics Disease Resistance Anthracnosis Poroto Variedad Paloma |
| url | https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-017-3685-7 http://hdl.handle.net/20.500.12123/3488 https://doi.org/10.1186/s12864-017-3685-7 |
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