Biocontrol of Sclerotinia sclerotiorum (Lib.) de Bary on common bean by native lipopeptide-producer Bacillus strains
Bacillus sp. B19, Bacillus sp. P12 and B. amyloliquefaciens B14 were isolated from soils of Salta province, and PGPR properties on the common bean (Phaseolus vulgaris L.) cv. Alubia and antagonistic activity against Sclerotinia sclerotiorum were studied. It was determined that B19 and P12 increased...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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2018
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| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S0944501317310546?via%3Dihub http://hdl.handle.net/20.500.12123/3487 https://doi.org/10.1016/j.micres.2018.04.003 |
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| author | Sabate, Daniela Constanza Perez Brandan, Carolina Petroselli, Gabriela Erra Balsells, Rosa Audisio, Marcela Carina |
| author_browse | Audisio, Marcela Carina Erra Balsells, Rosa Perez Brandan, Carolina Petroselli, Gabriela Sabate, Daniela Constanza |
| author_facet | Sabate, Daniela Constanza Perez Brandan, Carolina Petroselli, Gabriela Erra Balsells, Rosa Audisio, Marcela Carina |
| author_sort | Sabate, Daniela Constanza |
| collection | INTA Digital |
| description | Bacillus sp. B19, Bacillus sp. P12 and B. amyloliquefaciens B14 were isolated from soils of Salta province, and PGPR properties on the common bean (Phaseolus vulgaris L.) cv. Alubia and antagonistic activity against Sclerotinia sclerotiorum were studied.
It was determined that B19 and P12 increased crop germination potential (GP) from the common bean by 14.5% compared to control seeds; these strains also increased root length (10.4 and 15%, respectively) and stem length (20.2 and 30%, respectively) compared to the control; however, as for the B14 strain, no increases in growth parameters were detected. In addition, all the treatments that combined two bacilli: B14 + B19, B14 + P12 and B19 + P12, generated beneficial effects on GP and seedling growth compared to control seeds, but not compared to a single inoculant. B19 and P12 strains synthesized auxins at concentrations of 5.71 and 4.90 mg/mL, respectively, and it was qualitatively determined that they synthesize siderophores. In addition, previous studies have determined that B14 produces auxins in a concentration of 10.10 mg/mL, and qualitatively synthesizes siderophores.
The phytosanitary state of the white bean cv. Alubia control seeds revealed bacterial contamination in 87% of all the evaluated seeds and different fungi such as Cladosporium sp., Fusarium sp., and Rhizopus sp. Bean seeds treated with B14, B19 or P12 showed no growth of contaminating bacteria or of pathogenic fungi; in fact, bacilli inoculum development was observed in all seeds. Additionally, B19, P12 and B14 strains inhibited in vitro the development of 9 native S. sclerotiorum strains isolated from the Salta region, with FI ranging between 60 and 100%. The three Bacillus strains synthesized different isoforms of the lipopeptides: surfactin, iturin, and fengycin in the presence of S. sclerotiorum, as determined by MALDI-TOF.
In the in vivo trials, when common bean seeds were grown in soils contaminated with S. sclerotiorum, an incidence of 100% was determined when the seeds were not treated with any Bacillus. Seeds treated with the chemical fungicide and sown in S. sclerotiorum-infested soil did not produce seed emergence, while the inoculation of the seeds with B14 + P12, B14 + B19 or B19 + P12 reduced the effect of the pathogen by 46, 43 and 25%, respectively. Disease progression in B14 + P12 and B14 + B19 treatments was significantly lower than in the remaining treatments, with an AUDPC of 873.75 and 1071, respectively. |
| format | Artículo |
| id | INTA3487 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA34872018-09-27T11:58:05Z Biocontrol of Sclerotinia sclerotiorum (Lib.) de Bary on common bean by native lipopeptide-producer Bacillus strains Sabate, Daniela Constanza Perez Brandan, Carolina Petroselli, Gabriela Erra Balsells, Rosa Audisio, Marcela Carina Sclerotinia sclerotiorum Control Biológico Fríjol (phaseolus) Phaseolus vulgaris Bacillus Sideróforos Biological Control Kidney Beans Siderophores Poroto Bacillus sp. B19, Bacillus sp. P12 and B. amyloliquefaciens B14 were isolated from soils of Salta province, and PGPR properties on the common bean (Phaseolus vulgaris L.) cv. Alubia and antagonistic activity against Sclerotinia sclerotiorum were studied. It was determined that B19 and P12 increased crop germination potential (GP) from the common bean by 14.5% compared to control seeds; these strains also increased root length (10.4 and 15%, respectively) and stem length (20.2 and 30%, respectively) compared to the control; however, as for the B14 strain, no increases in growth parameters were detected. In addition, all the treatments that combined two bacilli: B14 + B19, B14 + P12 and B19 + P12, generated beneficial effects on GP and seedling growth compared to control seeds, but not compared to a single inoculant. B19 and P12 strains synthesized auxins at concentrations of 5.71 and 4.90 mg/mL, respectively, and it was qualitatively determined that they synthesize siderophores. In addition, previous studies have determined that B14 produces auxins in a concentration of 10.10 mg/mL, and qualitatively synthesizes siderophores. The phytosanitary state of the white bean cv. Alubia control seeds revealed bacterial contamination in 87% of all the evaluated seeds and different fungi such as Cladosporium sp., Fusarium sp., and Rhizopus sp. Bean seeds treated with B14, B19 or P12 showed no growth of contaminating bacteria or of pathogenic fungi; in fact, bacilli inoculum development was observed in all seeds. Additionally, B19, P12 and B14 strains inhibited in vitro the development of 9 native S. sclerotiorum strains isolated from the Salta region, with FI ranging between 60 and 100%. The three Bacillus strains synthesized different isoforms of the lipopeptides: surfactin, iturin, and fengycin in the presence of S. sclerotiorum, as determined by MALDI-TOF. In the in vivo trials, when common bean seeds were grown in soils contaminated with S. sclerotiorum, an incidence of 100% was determined when the seeds were not treated with any Bacillus. Seeds treated with the chemical fungicide and sown in S. sclerotiorum-infested soil did not produce seed emergence, while the inoculation of the seeds with B14 + P12, B14 + B19 or B19 + P12 reduced the effect of the pathogen by 46, 43 and 25%, respectively. Disease progression in B14 + P12 and B14 + B19 treatments was significantly lower than in the remaining treatments, with an AUDPC of 873.75 and 1071, respectively. EEA Salta Fil: Sabate, Daniela Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química; Argentina. Universidad Nacional de Salta. Instituto de Investigación para la Industria Química; Argentina Fil: Pérez Brandan, Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Petroselli, Gabriela. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones En Hidratos de Carbono; Argentina Fil: Erra Balsells, Rosa. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones En Hidratos de Carbono; Argentina Fil: Audisio, Marcela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química; Argentina. Universidad Nacional de Salta. Instituto de Investigación para la Industria Química; Argentina 2018-09-27T11:55:23Z 2018-09-27T11:55:23Z 2018-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0944501317310546?via%3Dihub http://hdl.handle.net/20.500.12123/3487 0944-5013 1618-0623 https://doi.org/10.1016/j.micres.2018.04.003 eng info:eu-repo/semantics/restrictedAccess application/pdf Microbiological Research 211 : 21-30 (June 2018) |
| spellingShingle | Sclerotinia sclerotiorum Control Biológico Fríjol (phaseolus) Phaseolus vulgaris Bacillus Sideróforos Biological Control Kidney Beans Siderophores Poroto Sabate, Daniela Constanza Perez Brandan, Carolina Petroselli, Gabriela Erra Balsells, Rosa Audisio, Marcela Carina Biocontrol of Sclerotinia sclerotiorum (Lib.) de Bary on common bean by native lipopeptide-producer Bacillus strains |
| title | Biocontrol of Sclerotinia sclerotiorum (Lib.) de Bary on common bean by native lipopeptide-producer Bacillus strains |
| title_full | Biocontrol of Sclerotinia sclerotiorum (Lib.) de Bary on common bean by native lipopeptide-producer Bacillus strains |
| title_fullStr | Biocontrol of Sclerotinia sclerotiorum (Lib.) de Bary on common bean by native lipopeptide-producer Bacillus strains |
| title_full_unstemmed | Biocontrol of Sclerotinia sclerotiorum (Lib.) de Bary on common bean by native lipopeptide-producer Bacillus strains |
| title_short | Biocontrol of Sclerotinia sclerotiorum (Lib.) de Bary on common bean by native lipopeptide-producer Bacillus strains |
| title_sort | biocontrol of sclerotinia sclerotiorum lib de bary on common bean by native lipopeptide producer bacillus strains |
| topic | Sclerotinia sclerotiorum Control Biológico Fríjol (phaseolus) Phaseolus vulgaris Bacillus Sideróforos Biological Control Kidney Beans Siderophores Poroto |
| url | https://www.sciencedirect.com/science/article/pii/S0944501317310546?via%3Dihub http://hdl.handle.net/20.500.12123/3487 https://doi.org/10.1016/j.micres.2018.04.003 |
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