Alfalfa snakin-1 prevents fungal colonization and probably coevolved with rhizobia
Background: The production of antimicrobial peptides is a common defense strategy of living cells against a wide range of pathogens. Plant snakin peptides inhibit bacterial and fungal growth at extremely low concentrations. However, little is known of their molecular and ecological characteristics,...
| Autores principales: | , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
BMC Plant Biology
2018
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| Materias: | |
| Acceso en línea: | https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-014-0248-9 http://hdl.handle.net/20.500.12123/3438 https://doi.org/10.1186/s12870-014-0248-9 |
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| author | Garcia, Araceli Nora Ayub, Nicolás Daniel Fox, Ana Romina Gomez, Maria Cristina Dieguez, Maria Jose Pagano, Elba Maria Berini, Carolina Andrea Muschietti, Jorge Prometeo Soto, Gabriela Cinthia |
| author_browse | Ayub, Nicolás Daniel Berini, Carolina Andrea Dieguez, Maria Jose Fox, Ana Romina Garcia, Araceli Nora Gomez, Maria Cristina Muschietti, Jorge Prometeo Pagano, Elba Maria Soto, Gabriela Cinthia |
| author_facet | Garcia, Araceli Nora Ayub, Nicolás Daniel Fox, Ana Romina Gomez, Maria Cristina Dieguez, Maria Jose Pagano, Elba Maria Berini, Carolina Andrea Muschietti, Jorge Prometeo Soto, Gabriela Cinthia |
| author_sort | Garcia, Araceli Nora |
| collection | INTA Digital |
| description | Background: The production of antimicrobial peptides is a common defense strategy of living cells against a wide range of pathogens. Plant snakin peptides inhibit bacterial and fungal growth at extremely low concentrations. However, little is known of their molecular and ecological characteristics, including origin, evolutionary equivalence, specific functions and activity against beneficial microbes. The aim of this study was to identify and characterize snakin-1 from alfalfa (MsSN1).
Results: Phylogenetic analysis showed complete congruence between snakin-1 and plant trees. The antimicrobial activity of MsSN1 against bacterial and fungal pathogens of alfalfa was demonstrated in vitro and in vivo. Transgenic alfalfa overexpressing MsSN1 showed increased antimicrobial activity against virulent fungal strains. However, MsSN1 did not affect nitrogen-fixing bacterial strains only when these had an alfalfa origin.
Conclusions: The results reported here suggest that snakin peptides have important and ancestral roles in land plant innate immunity. Our data indicate a coevolutionary process, in which alfalfa exerts a selection pressure for resistance to MsSN1 on rhizobial bacteria. The increased antimicrobial activity against virulent fungal strains without altering the nitrogen-fixing symbiosis observed in MsSN1-overexpressing alfalfa transgenic plants opens the way to the production of effective legume transgenic cultivars for biotic stress resistance. |
| format | info:ar-repo/semantics/artículo |
| id | INTA3438 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | BMC Plant Biology |
| publisherStr | BMC Plant Biology |
| record_format | dspace |
| spelling | INTA34382018-09-21T11:18:36Z Alfalfa snakin-1 prevents fungal colonization and probably coevolved with rhizobia Garcia, Araceli Nora Ayub, Nicolás Daniel Fox, Ana Romina Gomez, Maria Cristina Dieguez, Maria Jose Pagano, Elba Maria Berini, Carolina Andrea Muschietti, Jorge Prometeo Soto, Gabriela Cinthia Medicago sativa Péptidos Antimicrobianos Resistencia a la Enfermedad Peptides Antimicrobials Disease Resistance Alfalfa Background: The production of antimicrobial peptides is a common defense strategy of living cells against a wide range of pathogens. Plant snakin peptides inhibit bacterial and fungal growth at extremely low concentrations. However, little is known of their molecular and ecological characteristics, including origin, evolutionary equivalence, specific functions and activity against beneficial microbes. The aim of this study was to identify and characterize snakin-1 from alfalfa (MsSN1). Results: Phylogenetic analysis showed complete congruence between snakin-1 and plant trees. The antimicrobial activity of MsSN1 against bacterial and fungal pathogens of alfalfa was demonstrated in vitro and in vivo. Transgenic alfalfa overexpressing MsSN1 showed increased antimicrobial activity against virulent fungal strains. However, MsSN1 did not affect nitrogen-fixing bacterial strains only when these had an alfalfa origin. Conclusions: The results reported here suggest that snakin peptides have important and ancestral roles in land plant innate immunity. Our data indicate a coevolutionary process, in which alfalfa exerts a selection pressure for resistance to MsSN1 on rhizobial bacteria. The increased antimicrobial activity against virulent fungal strains without altering the nitrogen-fixing symbiosis observed in MsSN1-overexpressing alfalfa transgenic plants opens the way to the production of effective legume transgenic cultivars for biotic stress resistance. Instituto de Genética Fil: Garcia, Araceli Nora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Fox, Ana Romina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Fil: Gomez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Dieguez, María José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Pagano, Elba Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Berini, Carolina Andrea. Instituto de Investigaciones Biomédicas en Retrovirus y SIDA; Argentina Fil: Muschietti, Jorge Prometeo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, “Dr. Hector Torres”; Argentina Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina 2018-09-21T11:13:41Z 2018-09-21T11:13:41Z 2014-09-17 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-014-0248-9 http://hdl.handle.net/20.500.12123/3438 1471-2229 https://doi.org/10.1186/s12870-014-0248-9 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf BMC Plant Biology BMC Plant Biology 14: 248 (Sep 2014) |
| spellingShingle | Medicago sativa Péptidos Antimicrobianos Resistencia a la Enfermedad Peptides Antimicrobials Disease Resistance Alfalfa Garcia, Araceli Nora Ayub, Nicolás Daniel Fox, Ana Romina Gomez, Maria Cristina Dieguez, Maria Jose Pagano, Elba Maria Berini, Carolina Andrea Muschietti, Jorge Prometeo Soto, Gabriela Cinthia Alfalfa snakin-1 prevents fungal colonization and probably coevolved with rhizobia |
| title | Alfalfa snakin-1 prevents fungal colonization and probably coevolved with rhizobia |
| title_full | Alfalfa snakin-1 prevents fungal colonization and probably coevolved with rhizobia |
| title_fullStr | Alfalfa snakin-1 prevents fungal colonization and probably coevolved with rhizobia |
| title_full_unstemmed | Alfalfa snakin-1 prevents fungal colonization and probably coevolved with rhizobia |
| title_short | Alfalfa snakin-1 prevents fungal colonization and probably coevolved with rhizobia |
| title_sort | alfalfa snakin 1 prevents fungal colonization and probably coevolved with rhizobia |
| topic | Medicago sativa Péptidos Antimicrobianos Resistencia a la Enfermedad Peptides Antimicrobials Disease Resistance Alfalfa |
| url | https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-014-0248-9 http://hdl.handle.net/20.500.12123/3438 https://doi.org/10.1186/s12870-014-0248-9 |
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