Silencing of the glutathione biosynthetic pathway inhibits somatic embryogenesis in wheat
Somatic embryogenesis in scutella of wheat (Triticum aestivum L.) is a well documented phenomenon and it has been shown through transcriptome analysis that genes involved in antioxidant responses, particularly in glutathione (GSH) biosynthesis, participate in the process. Thus, we investigated the i...
| Autores principales: | , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2018
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/3432 https://link.springer.com/article/10.1007/s11240-012-0228-4 https://doi.org/10.1007/s11240-012-0228-4 |
| _version_ | 1855035141548670976 |
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| author | Bossio, Adrian Ezequiel Diaz Paleo, Antonio Horacio Del Vas, Mariana Baroli, Irene Acevedo, Alberto Rios, Raul Daniel |
| author_browse | Acevedo, Alberto Baroli, Irene Bossio, Adrian Ezequiel Del Vas, Mariana Diaz Paleo, Antonio Horacio Rios, Raul Daniel |
| author_facet | Bossio, Adrian Ezequiel Diaz Paleo, Antonio Horacio Del Vas, Mariana Baroli, Irene Acevedo, Alberto Rios, Raul Daniel |
| author_sort | Bossio, Adrian Ezequiel |
| collection | INTA Digital |
| description | Somatic embryogenesis in scutella of wheat (Triticum aestivum L.) is a well documented phenomenon and it has been shown through transcriptome analysis that genes involved in antioxidant responses, particularly in glutathione (GSH) biosynthesis, participate in the process. Thus, we investigated the influence of post-transcriptional silencing (PTGS) of the glutathione biosynthesis genes GSH1 and GSH2 on somatic embryogenesis in wheat. We found that PTGS of either of the target genes drastically inhibits callus regeneration and overall efficiency of transformation, in a similar manner as the GSH biosynthetic inhibitor buthionine sulfoximine. Supplementing the medium with glutathione did not overcome the observed low efficiency of wheat transformation. Furthermore, of the small number of obtained transformants, none exhibited altered GSH1 and GSH2 levels of transcription. Thus, it is concluded that GSH is essential for somatic embryogenesis and, as a consequence, it is difficult to regenerate wheat plants with silenced GSH1 and GSH2 genes. |
| format | info:ar-repo/semantics/artículo |
| id | INTA3432 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA34322021-11-11T12:06:01Z Silencing of the glutathione biosynthetic pathway inhibits somatic embryogenesis in wheat Bossio, Adrian Ezequiel Diaz Paleo, Antonio Horacio Del Vas, Mariana Baroli, Irene Acevedo, Alberto Rios, Raul Daniel Embriogénesis Somática Trigo Triticum aestivum Biosíntesis Glutation Transcripción Génica Somatic Embryogenesis Wheat Bioshyntesis Glutathione Gene Transcription Silenciamiento postranscripcional Somatic embryogenesis in scutella of wheat (Triticum aestivum L.) is a well documented phenomenon and it has been shown through transcriptome analysis that genes involved in antioxidant responses, particularly in glutathione (GSH) biosynthesis, participate in the process. Thus, we investigated the influence of post-transcriptional silencing (PTGS) of the glutathione biosynthesis genes GSH1 and GSH2 on somatic embryogenesis in wheat. We found that PTGS of either of the target genes drastically inhibits callus regeneration and overall efficiency of transformation, in a similar manner as the GSH biosynthetic inhibitor buthionine sulfoximine. Supplementing the medium with glutathione did not overcome the observed low efficiency of wheat transformation. Furthermore, of the small number of obtained transformants, none exhibited altered GSH1 and GSH2 levels of transcription. Thus, it is concluded that GSH is essential for somatic embryogenesis and, as a consequence, it is difficult to regenerate wheat plants with silenced GSH1 and GSH2 genes. Instituto de Genética Fil: Bossio, Adrian Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina Fil: Diaz Paleo, Antonio Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina Fil: Del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Fil: Baroli, Irene. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Acevedo, Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación de Recursos Naturales; Argentina Fil: Ríos, Raúl Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina 2018-09-20T17:25:42Z 2018-09-20T17:25:42Z 2013-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/3432 https://link.springer.com/article/10.1007/s11240-012-0228-4 1573-5044 https://doi.org/10.1007/s11240-012-0228-4 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Plant Cell, Tissue and Organ Culture 112 (2) : 239–248 (February 2013) |
| spellingShingle | Embriogénesis Somática Trigo Triticum aestivum Biosíntesis Glutation Transcripción Génica Somatic Embryogenesis Wheat Bioshyntesis Glutathione Gene Transcription Silenciamiento postranscripcional Bossio, Adrian Ezequiel Diaz Paleo, Antonio Horacio Del Vas, Mariana Baroli, Irene Acevedo, Alberto Rios, Raul Daniel Silencing of the glutathione biosynthetic pathway inhibits somatic embryogenesis in wheat |
| title | Silencing of the glutathione biosynthetic pathway inhibits somatic embryogenesis in wheat |
| title_full | Silencing of the glutathione biosynthetic pathway inhibits somatic embryogenesis in wheat |
| title_fullStr | Silencing of the glutathione biosynthetic pathway inhibits somatic embryogenesis in wheat |
| title_full_unstemmed | Silencing of the glutathione biosynthetic pathway inhibits somatic embryogenesis in wheat |
| title_short | Silencing of the glutathione biosynthetic pathway inhibits somatic embryogenesis in wheat |
| title_sort | silencing of the glutathione biosynthetic pathway inhibits somatic embryogenesis in wheat |
| topic | Embriogénesis Somática Trigo Triticum aestivum Biosíntesis Glutation Transcripción Génica Somatic Embryogenesis Wheat Bioshyntesis Glutathione Gene Transcription Silenciamiento postranscripcional |
| url | http://hdl.handle.net/20.500.12123/3432 https://link.springer.com/article/10.1007/s11240-012-0228-4 https://doi.org/10.1007/s11240-012-0228-4 |
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