Microsatellite markers from the ‘South American fruit fly’ Anastrepha fraterculusa: valuable tool for population genetic analysis and SIT applications
Background: Anastrepha fraterculus Wiedemann is a horticultural pest which causes significant economic losses in the fruit-producing areas of the American continent and limits the access of products to international markets. The use of environmentally friendly control strategies against this pest is...
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
BioMed Central
2018
|
| Materias: | |
| Acceso en línea: | https://bmcgenet.biomedcentral.com/articles/10.1186/1471-2156-15-S2-S13 http://hdl.handle.net/20.500.12123/3414 https://doi.org/10.1186/1471-2156-15-S2-S13 |
| _version_ | 1855035138585395200 |
|---|---|
| author | Lanzavecchia, Silvia Beatriz Juri, Marianela Lucía Bonomi, Angelica Gomulski, Ludvik Scannapieco, Alejandra Carla Segura, Diego Fernando Malacrida, Anna Cladera, Jorge Luis Gasperi, Giuliano |
| author_browse | Bonomi, Angelica Cladera, Jorge Luis Gasperi, Giuliano Gomulski, Ludvik Juri, Marianela Lucía Lanzavecchia, Silvia Beatriz Malacrida, Anna Scannapieco, Alejandra Carla Segura, Diego Fernando |
| author_facet | Lanzavecchia, Silvia Beatriz Juri, Marianela Lucía Bonomi, Angelica Gomulski, Ludvik Scannapieco, Alejandra Carla Segura, Diego Fernando Malacrida, Anna Cladera, Jorge Luis Gasperi, Giuliano |
| author_sort | Lanzavecchia, Silvia Beatriz |
| collection | INTA Digital |
| description | Background: Anastrepha fraterculus Wiedemann is a horticultural pest which causes significant economic losses in the fruit-producing areas of the American continent and limits the access of products to international markets. The use of environmentally friendly control strategies against this pest is constrained due to the limited knowledge of
its population structure.
Results: We developed microsatellite markers for A. fraterculus from four genomic libraries, which were enriched in CA, CAA, GA and CAT microsatellite motifs. Fifty microsatellite regions were evaluated and 14 loci were selected for population genetics studies. Genotypes of 122 individuals sampled from four A. fraterculus populations were analyzed. The level of polymorphism ranged from three to 13 alleles per locus and the mean expected heterozygosity ranged from 0.60 to 0.64. Comparison between allelic and genotypic frequencies showed significant
differences among all pairs of populations.
Conclusions: This novel set of microsatellite markers provides valuable information for the description of genetic variability and population structure of wild populations and laboratory strains of A. fraterculus. This information will be used to identify and characterize candidate strains suitable to implement effective pest control strategies and might represent a first step towards having a more comprehensive knowledge about the genetics of this pest. |
| format | info:ar-repo/semantics/artículo |
| id | INTA3414 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | BioMed Central |
| publisherStr | BioMed Central |
| record_format | dspace |
| spelling | INTA34142018-09-19T18:18:27Z Microsatellite markers from the ‘South American fruit fly’ Anastrepha fraterculusa: valuable tool for population genetic analysis and SIT applications Lanzavecchia, Silvia Beatriz Juri, Marianela Lucía Bonomi, Angelica Gomulski, Ludvik Scannapieco, Alejandra Carla Segura, Diego Fernando Malacrida, Anna Cladera, Jorge Luis Gasperi, Giuliano Marcadores Genéticos Tephritidae Anastrepha fraterculus Control Biológico Genetic Markers Biological Control Mosca de la fruta Técnica del insecto estéril Background: Anastrepha fraterculus Wiedemann is a horticultural pest which causes significant economic losses in the fruit-producing areas of the American continent and limits the access of products to international markets. The use of environmentally friendly control strategies against this pest is constrained due to the limited knowledge of its population structure. Results: We developed microsatellite markers for A. fraterculus from four genomic libraries, which were enriched in CA, CAA, GA and CAT microsatellite motifs. Fifty microsatellite regions were evaluated and 14 loci were selected for population genetics studies. Genotypes of 122 individuals sampled from four A. fraterculus populations were analyzed. The level of polymorphism ranged from three to 13 alleles per locus and the mean expected heterozygosity ranged from 0.60 to 0.64. Comparison between allelic and genotypic frequencies showed significant differences among all pairs of populations. Conclusions: This novel set of microsatellite markers provides valuable information for the description of genetic variability and population structure of wild populations and laboratory strains of A. fraterculus. This information will be used to identify and characterize candidate strains suitable to implement effective pest control strategies and might represent a first step towards having a more comprehensive knowledge about the genetics of this pest. Instituto de Genética Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina Fil: Juri, Marianela Lucía. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bonomi, Angelica. Universitá di Pavia, Dipartimento di Biologia e Biotecnologie ‘L. Spallanzani’; Italia Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina Fil: Gasperi, Giuliano. Universitá di Pavia, Dipartimento di Biologia e Biotecnologie ‘L. Spallanzani’; Italia Fil: Gomulski, Ludvik. Universitá di Pavia, Dipartimento di Biologia e Biotecnologie ‘L. Spallanzani’; Italia Fil: Malacrida, Anna. Universitá di Pavia, Dipartimento di Biologia e Biotecnologie ‘L. Spallanzani’; Italia 2018-09-19T18:10:32Z 2018-09-19T18:10:32Z 2014-12-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://bmcgenet.biomedcentral.com/articles/10.1186/1471-2156-15-S2-S13 http://hdl.handle.net/20.500.12123/3414 1471-2156 https://doi.org/10.1186/1471-2156-15-S2-S13 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf BioMed Central BMC Genetics 15 (Suppl 2) : S13 (2014) |
| spellingShingle | Marcadores Genéticos Tephritidae Anastrepha fraterculus Control Biológico Genetic Markers Biological Control Mosca de la fruta Técnica del insecto estéril Lanzavecchia, Silvia Beatriz Juri, Marianela Lucía Bonomi, Angelica Gomulski, Ludvik Scannapieco, Alejandra Carla Segura, Diego Fernando Malacrida, Anna Cladera, Jorge Luis Gasperi, Giuliano Microsatellite markers from the ‘South American fruit fly’ Anastrepha fraterculusa: valuable tool for population genetic analysis and SIT applications |
| title | Microsatellite markers from the ‘South American fruit fly’ Anastrepha fraterculusa: valuable tool for population genetic analysis and SIT applications |
| title_full | Microsatellite markers from the ‘South American fruit fly’ Anastrepha fraterculusa: valuable tool for population genetic analysis and SIT applications |
| title_fullStr | Microsatellite markers from the ‘South American fruit fly’ Anastrepha fraterculusa: valuable tool for population genetic analysis and SIT applications |
| title_full_unstemmed | Microsatellite markers from the ‘South American fruit fly’ Anastrepha fraterculusa: valuable tool for population genetic analysis and SIT applications |
| title_short | Microsatellite markers from the ‘South American fruit fly’ Anastrepha fraterculusa: valuable tool for population genetic analysis and SIT applications |
| title_sort | microsatellite markers from the south american fruit fly anastrepha fraterculusa valuable tool for population genetic analysis and sit applications |
| topic | Marcadores Genéticos Tephritidae Anastrepha fraterculus Control Biológico Genetic Markers Biological Control Mosca de la fruta Técnica del insecto estéril |
| url | https://bmcgenet.biomedcentral.com/articles/10.1186/1471-2156-15-S2-S13 http://hdl.handle.net/20.500.12123/3414 https://doi.org/10.1186/1471-2156-15-S2-S13 |
| work_keys_str_mv | AT lanzavecchiasilviabeatriz microsatellitemarkersfromthesouthamericanfruitflyanastrephafraterculusavaluabletoolforpopulationgeneticanalysisandsitapplications AT jurimarianelalucia microsatellitemarkersfromthesouthamericanfruitflyanastrephafraterculusavaluabletoolforpopulationgeneticanalysisandsitapplications AT bonomiangelica microsatellitemarkersfromthesouthamericanfruitflyanastrephafraterculusavaluabletoolforpopulationgeneticanalysisandsitapplications AT gomulskiludvik microsatellitemarkersfromthesouthamericanfruitflyanastrephafraterculusavaluabletoolforpopulationgeneticanalysisandsitapplications AT scannapiecoalejandracarla microsatellitemarkersfromthesouthamericanfruitflyanastrephafraterculusavaluabletoolforpopulationgeneticanalysisandsitapplications AT seguradiegofernando microsatellitemarkersfromthesouthamericanfruitflyanastrephafraterculusavaluabletoolforpopulationgeneticanalysisandsitapplications AT malacridaanna microsatellitemarkersfromthesouthamericanfruitflyanastrephafraterculusavaluabletoolforpopulationgeneticanalysisandsitapplications AT claderajorgeluis microsatellitemarkersfromthesouthamericanfruitflyanastrephafraterculusavaluabletoolforpopulationgeneticanalysisandsitapplications AT gasperigiuliano microsatellitemarkersfromthesouthamericanfruitflyanastrephafraterculusavaluabletoolforpopulationgeneticanalysisandsitapplications |