Absence of the nitrous oxide reductase gene cluster in commercial alfalfa inoculants is probably due to the extensive loss of genes during rhizobial domestication
As other legume crops, alfalfa cultivation increases the emission of the greenhouse gas nitrous oxide (N2O). Since legumesymbiotic nitrogen-fixing bacteria play a crucial role in this emission, it is important to understand the possible impacts of rhizobial domestication on the evolution of denitr...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Springer
2018
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| Online Access: | https://link.springer.com/article/10.1007/s00248-018-1145-9 http://hdl.handle.net/20.500.12123/3399 https://doi.org/10.1007/s00248-018-1145-9 |
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| author | Brambilla, Silvina Maricel Frare, Romina Alejandra Soto, Gabriela Cinthia Jozefkowicz, Cintia Ayub, Nicolás Daniel |
| author_browse | Ayub, Nicolás Daniel Brambilla, Silvina Maricel Frare, Romina Alejandra Jozefkowicz, Cintia Soto, Gabriela Cinthia |
| author_facet | Brambilla, Silvina Maricel Frare, Romina Alejandra Soto, Gabriela Cinthia Jozefkowicz, Cintia Ayub, Nicolás Daniel |
| author_sort | Brambilla, Silvina Maricel |
| collection | INTA Digital |
| description | As other legume crops, alfalfa cultivation increases the emission of the greenhouse gas nitrous oxide (N2O). Since legumesymbiotic
nitrogen-fixing bacteria play a crucial role in this emission, it is important to understand the possible impacts of
rhizobial domestication on the evolution of denitrification genes. In comparison with the genomes of non-commercial strains,
those of commercial alfalfa inoculants exhibit low total genome size, low number of ORFs and high numbers of both
frameshifted genes and pseudogenes, suggesting a dramatic loss of genes during bacterial domestication. Genomic analysis
focused on denitrification genes revealed that commercial strains have perfectly conserved the nitrate (NAP), nitrite (NIR) and
nitric (NOR) reductase clusters related to the production of N2O from nitrate but completely lost the nitrous oxide (NOS)
reductase cluster (nosRZDFYLX genes) associated with the reduction of N2O to gas nitrogen. Based on these results, we propose
future screenings for alfalfa-nodulating isolates containing both nitrogen fixation and N2O reductase genes for environmental
sustainability of alfalfa production. |
| format | info:ar-repo/semantics/artículo |
| id | INTA3399 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA33992018-09-19T17:10:15Z Absence of the nitrous oxide reductase gene cluster in commercial alfalfa inoculants is probably due to the extensive loss of genes during rhizobial domestication Brambilla, Silvina Maricel Frare, Romina Alejandra Soto, Gabriela Cinthia Jozefkowicz, Cintia Ayub, Nicolás Daniel Óxido Nitroso Medicago sativa Fijación del Nitrógeno Muestreo cluster Genes Rhizobium Nitrous Oxide Nitrogen Fixation Cluster Sampling Inoculantes comerciales As other legume crops, alfalfa cultivation increases the emission of the greenhouse gas nitrous oxide (N2O). Since legumesymbiotic nitrogen-fixing bacteria play a crucial role in this emission, it is important to understand the possible impacts of rhizobial domestication on the evolution of denitrification genes. In comparison with the genomes of non-commercial strains, those of commercial alfalfa inoculants exhibit low total genome size, low number of ORFs and high numbers of both frameshifted genes and pseudogenes, suggesting a dramatic loss of genes during bacterial domestication. Genomic analysis focused on denitrification genes revealed that commercial strains have perfectly conserved the nitrate (NAP), nitrite (NIR) and nitric (NOR) reductase clusters related to the production of N2O from nitrate but completely lost the nitrous oxide (NOS) reductase cluster (nosRZDFYLX genes) associated with the reduction of N2O to gas nitrogen. Based on these results, we propose future screenings for alfalfa-nodulating isolates containing both nitrogen fixation and N2O reductase genes for environmental sustainability of alfalfa production. Instituto de Genética Fil: Brambilla, Silvina Maricel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina Fil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Jozefkowicz, Cintia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2018-09-19T17:04:53Z 2018-09-19T17:04:53Z 2018-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007/s00248-018-1145-9 http://hdl.handle.net/20.500.12123/3399 0095-3628 1432-184X https://doi.org/10.1007/s00248-018-1145-9 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Microbial Ecology 76 (2): 299–302 (August 2018) |
| spellingShingle | Óxido Nitroso Medicago sativa Fijación del Nitrógeno Muestreo cluster Genes Rhizobium Nitrous Oxide Nitrogen Fixation Cluster Sampling Inoculantes comerciales Brambilla, Silvina Maricel Frare, Romina Alejandra Soto, Gabriela Cinthia Jozefkowicz, Cintia Ayub, Nicolás Daniel Absence of the nitrous oxide reductase gene cluster in commercial alfalfa inoculants is probably due to the extensive loss of genes during rhizobial domestication |
| title | Absence of the nitrous oxide reductase gene cluster in commercial alfalfa inoculants is probably due to the extensive loss of genes during rhizobial domestication |
| title_full | Absence of the nitrous oxide reductase gene cluster in commercial alfalfa inoculants is probably due to the extensive loss of genes during rhizobial domestication |
| title_fullStr | Absence of the nitrous oxide reductase gene cluster in commercial alfalfa inoculants is probably due to the extensive loss of genes during rhizobial domestication |
| title_full_unstemmed | Absence of the nitrous oxide reductase gene cluster in commercial alfalfa inoculants is probably due to the extensive loss of genes during rhizobial domestication |
| title_short | Absence of the nitrous oxide reductase gene cluster in commercial alfalfa inoculants is probably due to the extensive loss of genes during rhizobial domestication |
| title_sort | absence of the nitrous oxide reductase gene cluster in commercial alfalfa inoculants is probably due to the extensive loss of genes during rhizobial domestication |
| topic | Óxido Nitroso Medicago sativa Fijación del Nitrógeno Muestreo cluster Genes Rhizobium Nitrous Oxide Nitrogen Fixation Cluster Sampling Inoculantes comerciales |
| url | https://link.springer.com/article/10.1007/s00248-018-1145-9 http://hdl.handle.net/20.500.12123/3399 https://doi.org/10.1007/s00248-018-1145-9 |
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