Characterization of Fusarium species associated with tobacco diseases in Northwestern Argentina
Tobacco (Nicotiana tabacum L.) production is centred in the northwestern region of Argentina (NWA), where the incidence of root rot and stem diseases has increased considerably in recent years. This study aimed to analyse the genetic, morphological and pathogenic diversity of the Fusarium oxysporum...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/3385 https://link.springer.com/article/10.1007/s10658-018-1443-6 https://doi.org/10.1007/s10658-018-1443-6 |
| _version_ | 1855483232586301440 |
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| author | Berruezo, Lorena Andrea Mercado Cardenas, Guadalupe Eugenia Harries, Eleonora Del Milagro Stenglein, Sebastian Alberto Curti, Ramiro Nestor Rodriguero, Marcela Silvina Galvan, Marta Zulema |
| author_browse | Berruezo, Lorena Andrea Curti, Ramiro Nestor Galvan, Marta Zulema Harries, Eleonora Del Milagro Mercado Cardenas, Guadalupe Eugenia Rodriguero, Marcela Silvina Stenglein, Sebastian Alberto |
| author_facet | Berruezo, Lorena Andrea Mercado Cardenas, Guadalupe Eugenia Harries, Eleonora Del Milagro Stenglein, Sebastian Alberto Curti, Ramiro Nestor Rodriguero, Marcela Silvina Galvan, Marta Zulema |
| author_sort | Berruezo, Lorena Andrea |
| collection | INTA Digital |
| description | Tobacco (Nicotiana tabacum L.) production is centred in the northwestern region of Argentina (NWA), where the incidence of root rot and stem diseases has increased considerably in recent years. This study aimed to analyse the genetic, morphological and pathogenic diversity of the Fusarium oxysporum and F. solani complexes (hereafter FOSC-FSSC), causing Fusarium wilt and root rot. One hundred tobacco fields were surveyed at six locations during two consecutive seasons, and 130 isolates were recovered from symptomatic tobacco plants. The isolates were characterized by morphological traits, molecular characteristics (EF1-α sequence) and pathogenicity tests. All of the isolates were identified as members of the FOSC or FSSC, exhibiting considerable intra-group variation. Three morphotypes were differentiated based on morphological characters in both complexes. The phylogenetic tree generated from the EF1-α sequences confirmed the isolates’ identification. The pathogenicity of the isolates towards tobacco seedlings was assessed in a greenhouse. Considerable variability in pathogenicity was observed among the isolates. Differences in the levels of pathogenicity were recorded. In the FOSC and FSSC, 81% and 60% of the isolates were pathogenic, respectively. In this study, members of FOSC and FSSC exhibited considerable variability in morphological characteristics and virulence, and a portion of them were non-pathogenic for tobacco. To the best of our knowledge, this study is the first to provide information on the variability of the pathogens associated with tobacco wilt and root rot in NWA. This work contributes to the development of sustainable management strategies in tobacco production. |
| format | Artículo |
| id | INTA3385 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA33852025-05-19T10:09:24Z Characterization of Fusarium species associated with tobacco diseases in Northwestern Argentina Berruezo, Lorena Andrea Mercado Cardenas, Guadalupe Eugenia Harries, Eleonora Del Milagro Stenglein, Sebastian Alberto Curti, Ramiro Nestor Rodriguero, Marcela Silvina Galvan, Marta Zulema Tabaco Nicotiana tabacum Enfermedades de las Plantas Fusarium Patogenicidad Podredumbre de la Raíz Marchitez Tobacco Plant Diseases Pathogenicity Root Rots Wilts Tabaco (planta) Región Noroeste, Argentina Tobacco (Nicotiana tabacum L.) production is centred in the northwestern region of Argentina (NWA), where the incidence of root rot and stem diseases has increased considerably in recent years. This study aimed to analyse the genetic, morphological and pathogenic diversity of the Fusarium oxysporum and F. solani complexes (hereafter FOSC-FSSC), causing Fusarium wilt and root rot. One hundred tobacco fields were surveyed at six locations during two consecutive seasons, and 130 isolates were recovered from symptomatic tobacco plants. The isolates were characterized by morphological traits, molecular characteristics (EF1-α sequence) and pathogenicity tests. All of the isolates were identified as members of the FOSC or FSSC, exhibiting considerable intra-group variation. Three morphotypes were differentiated based on morphological characters in both complexes. The phylogenetic tree generated from the EF1-α sequences confirmed the isolates’ identification. The pathogenicity of the isolates towards tobacco seedlings was assessed in a greenhouse. Considerable variability in pathogenicity was observed among the isolates. Differences in the levels of pathogenicity were recorded. In the FOSC and FSSC, 81% and 60% of the isolates were pathogenic, respectively. In this study, members of FOSC and FSSC exhibited considerable variability in morphological characteristics and virulence, and a portion of them were non-pathogenic for tobacco. To the best of our knowledge, this study is the first to provide information on the variability of the pathogens associated with tobacco wilt and root rot in NWA. This work contributes to the development of sustainable management strategies in tobacco production. EEA Salta Fil: Berruezo, Lorena Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Mercado Cardenas, Guadalupe Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina Fil: Harries, Eleonora Del Milagro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina Fil: Stenglein, Sebastian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biologia Funcional y Biotecnología; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomia; Argentina Fil: Curti, Ramiro Nestor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina Fil: Rodriguero, Marcela Silvina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Galvan, Marta Zulema. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina 2018-09-18T13:39:58Z 2018-09-18T13:39:58Z 2018-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/3385 https://link.springer.com/article/10.1007/s10658-018-1443-6 0929-1873 1573-8469 https://doi.org/10.1007/s10658-018-1443-6 eng info:eu-repo/semantics/restrictedAccess application/pdf European Journal of Plant Pathology 151 (4) : 1065–1079 (August 2018) |
| spellingShingle | Tabaco Nicotiana tabacum Enfermedades de las Plantas Fusarium Patogenicidad Podredumbre de la Raíz Marchitez Tobacco Plant Diseases Pathogenicity Root Rots Wilts Tabaco (planta) Región Noroeste, Argentina Berruezo, Lorena Andrea Mercado Cardenas, Guadalupe Eugenia Harries, Eleonora Del Milagro Stenglein, Sebastian Alberto Curti, Ramiro Nestor Rodriguero, Marcela Silvina Galvan, Marta Zulema Characterization of Fusarium species associated with tobacco diseases in Northwestern Argentina |
| title | Characterization of Fusarium species associated with tobacco diseases in Northwestern Argentina |
| title_full | Characterization of Fusarium species associated with tobacco diseases in Northwestern Argentina |
| title_fullStr | Characterization of Fusarium species associated with tobacco diseases in Northwestern Argentina |
| title_full_unstemmed | Characterization of Fusarium species associated with tobacco diseases in Northwestern Argentina |
| title_short | Characterization of Fusarium species associated with tobacco diseases in Northwestern Argentina |
| title_sort | characterization of fusarium species associated with tobacco diseases in northwestern argentina |
| topic | Tabaco Nicotiana tabacum Enfermedades de las Plantas Fusarium Patogenicidad Podredumbre de la Raíz Marchitez Tobacco Plant Diseases Pathogenicity Root Rots Wilts Tabaco (planta) Región Noroeste, Argentina |
| url | http://hdl.handle.net/20.500.12123/3385 https://link.springer.com/article/10.1007/s10658-018-1443-6 https://doi.org/10.1007/s10658-018-1443-6 |
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