Rhipicephalus sanguineus (Latreille, 1806): Neotype designation, morphological re-description of all parasitic stages and molecular characterization

The aims of this work were to re-describe all parasitic stages of Rhipicephalus sanguineus sensu stricto, to select and deposit a neotype, and to characterize some of its diagnostic molecular traits. A male of R. sanguineus s.s. collected in Montpellier, France, was designated as neotype. The diagno...

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Autores principales: Nava, Santiago, Beati, Lorenza, Venzal, José Manuel, Labruna, Marcelo B., Szabó, Matías P.J., Petney, Trevor, Saracho Bottero, Maria Noelia, Tarragona, Evelina Luisa, Dantas-Torres, Filipe, Silva, Maria M. Santos, Mangold, Atilio Jose, Guglielmone, Alberto, Estrada-Peña, Agustín
Formato: info:ar-repo/semantics/artículo
Lenguaje:Inglés
Publicado: 2018
Materias:
Acceso en línea:https://www.sciencedirect.com/science/article/pii/S1877959X18302152?via%3Dihub
http://hdl.handle.net/20.500.12123/3381
https://doi.org/10.1016/j.ttbdis.2018.08.001
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author Nava, Santiago
Beati, Lorenza
Venzal, José Manuel
Labruna, Marcelo B.
Szabó, Matías P.J.
Petney, Trevor
Saracho Bottero, Maria Noelia
Tarragona, Evelina Luisa
Dantas-Torres, Filipe
Silva, Maria M. Santos
Mangold, Atilio Jose
Guglielmone, Alberto
Estrada-Peña, Agustín
author_browse Beati, Lorenza
Dantas-Torres, Filipe
Estrada-Peña, Agustín
Guglielmone, Alberto
Labruna, Marcelo B.
Mangold, Atilio Jose
Nava, Santiago
Petney, Trevor
Saracho Bottero, Maria Noelia
Silva, Maria M. Santos
Szabó, Matías P.J.
Tarragona, Evelina Luisa
Venzal, José Manuel
author_facet Nava, Santiago
Beati, Lorenza
Venzal, José Manuel
Labruna, Marcelo B.
Szabó, Matías P.J.
Petney, Trevor
Saracho Bottero, Maria Noelia
Tarragona, Evelina Luisa
Dantas-Torres, Filipe
Silva, Maria M. Santos
Mangold, Atilio Jose
Guglielmone, Alberto
Estrada-Peña, Agustín
author_sort Nava, Santiago
collection INTA Digital
description The aims of this work were to re-describe all parasitic stages of Rhipicephalus sanguineus sensu stricto, to select and deposit a neotype, and to characterize some of its diagnostic molecular traits. A male of R. sanguineus s.s. collected in Montpellier, France, was designated as neotype. The diagnostic characters unique to the male of R. sanguineus s.s. are: spiracular plate elongated and subtriangular in shape with a dorsal prolongation narrow and usually visible dorsally, with the dorsal prolongation narrower than the width of the adjacent festoon; punctations of the scutum moderate in number and unequal in size; marginal groove conspicuous, deep and punctate; posteromedian groove distinct and elongated, and posterolateral grooves often sub-circular, shorter than posteromedian groove; adanal plates long, wide, and subtriangular in shape, with a clear concavity in its inner margin and posterior margin broadly rounded or truncated; accessory adanal plates with the posterior end pointed, narrower than the width of adjacent festoon. The female of R. sanguineus s.s. can be diagnosed by a combination of broadly U-shaped genital aperture, spiracular plate with a narrow dorsal prolongation visible dorsally, basis capituli hexagonal with broad lateral angles, and scutum barely longer than broad with posterior margin sinuous and punctations moderate in number and unequal in size, larger and more numerous along cervical fields. The nymph has a basis capituli sub-triangular dorsally with lateral angles slightly curved and presence of ventral processes, scutum approximately as long as broad with lateral margins nearly straights, posterior margin broadly rounded, and cervical grooves short and sigmoid in shape extending posteriorly to the level of the eyes. The larva is characterized by basis capituli broader than long with lateral angles short and slightly curved and with posterior margin slightly convex, cervical grooves short, shallow and subparallel, and scutum almost twice broader than long. The phylogenetic analysis of DNA sequences support R. sanguineus s.s. as a well-defined taxon when compared with other species of the R. sanguineus group: R. turanicus s.s., R. camicasi, R. guilhoni, R. sulcatus, R. pusillus, R. rossicus and R. leporis. Molecularly R. sanguineus s.s. also encompasses the so-called “temperate lineage” from the New World (Argentina, southern Brazil, Chile, Uruguay, and USA). The evidence currently available supports the presence of R. sanguineus s.s. in Europe (France, Italy, Spain, Switzerland and Portugal) and America (Argentina, Brazil, Chile, Uruguay and U.S.A.), but further studies are needed to determine the exact geographic range of this taxon.
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spelling INTA33812018-09-18T12:19:11Z Rhipicephalus sanguineus (Latreille, 1806): Neotype designation, morphological re-description of all parasitic stages and molecular characterization Nava, Santiago Beati, Lorenza Venzal, José Manuel Labruna, Marcelo B. Szabó, Matías P.J. Petney, Trevor Saracho Bottero, Maria Noelia Tarragona, Evelina Luisa Dantas-Torres, Filipe Silva, Maria M. Santos Mangold, Atilio Jose Guglielmone, Alberto Estrada-Peña, Agustín Rhipicephalus Sanguineus Metastigmata Identificación Anatomía Animal Secuencia Nucleotídica Identification Animal Morphology Nucleotide Sequence Garrapatas The aims of this work were to re-describe all parasitic stages of Rhipicephalus sanguineus sensu stricto, to select and deposit a neotype, and to characterize some of its diagnostic molecular traits. A male of R. sanguineus s.s. collected in Montpellier, France, was designated as neotype. The diagnostic characters unique to the male of R. sanguineus s.s. are: spiracular plate elongated and subtriangular in shape with a dorsal prolongation narrow and usually visible dorsally, with the dorsal prolongation narrower than the width of the adjacent festoon; punctations of the scutum moderate in number and unequal in size; marginal groove conspicuous, deep and punctate; posteromedian groove distinct and elongated, and posterolateral grooves often sub-circular, shorter than posteromedian groove; adanal plates long, wide, and subtriangular in shape, with a clear concavity in its inner margin and posterior margin broadly rounded or truncated; accessory adanal plates with the posterior end pointed, narrower than the width of adjacent festoon. The female of R. sanguineus s.s. can be diagnosed by a combination of broadly U-shaped genital aperture, spiracular plate with a narrow dorsal prolongation visible dorsally, basis capituli hexagonal with broad lateral angles, and scutum barely longer than broad with posterior margin sinuous and punctations moderate in number and unequal in size, larger and more numerous along cervical fields. The nymph has a basis capituli sub-triangular dorsally with lateral angles slightly curved and presence of ventral processes, scutum approximately as long as broad with lateral margins nearly straights, posterior margin broadly rounded, and cervical grooves short and sigmoid in shape extending posteriorly to the level of the eyes. The larva is characterized by basis capituli broader than long with lateral angles short and slightly curved and with posterior margin slightly convex, cervical grooves short, shallow and subparallel, and scutum almost twice broader than long. The phylogenetic analysis of DNA sequences support R. sanguineus s.s. as a well-defined taxon when compared with other species of the R. sanguineus group: R. turanicus s.s., R. camicasi, R. guilhoni, R. sulcatus, R. pusillus, R. rossicus and R. leporis. Molecularly R. sanguineus s.s. also encompasses the so-called “temperate lineage” from the New World (Argentina, southern Brazil, Chile, Uruguay, and USA). The evidence currently available supports the presence of R. sanguineus s.s. in Europe (France, Italy, Spain, Switzerland and Portugal) and America (Argentina, Brazil, Chile, Uruguay and U.S.A.), but further studies are needed to determine the exact geographic range of this taxon. EEA Rafaela Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Beati, Lorenza. Georgia Southern University. Institute for Coastal Plain Science. United States National Tick Collection; Estados Unidos Fil: Venzal, José Manuel. Universidad de la República. CENUR Litoral Norte-Salto. Facultad de Veterinaria. Laboratorio de Vectores y Enfermedades Transmitidas; Uruguay Fil: Labruna, Marcelo B. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; Brasil Fil: Szabó, Matías P.J. Universidade Federal de Uberlandia. Faculdade de Medicina Veterinaria; Brasil Fil: Petney, Trevor. Karlsruhe Institute of Technology. Department of Ecology and Parasitology; Alemania Fil: Saracho Bottero, Maria Noelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Tarragona, Evelina Luisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Dantas-Torres, Filipe. Oswaldo Cruz Foundation (Fiocruz). Aggeu Magalhães Institute. Department of Immunology; Brasil Fil: Silva, Maria M. Santos. Instituto Nacional de Saúde Dr. Ricardo Jorge. Centro de Estudos de Vectores e Doenças Infecciosas Dr. Francisco Cambournac; Portugal Fil: Mangold, Atilio Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Guglielmone, Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Estrada-Peña, Agustin. Universidad de Zaragoza. Facultad de Veterinaria. Departamento de Patología Animal; España 2018-09-18T12:16:59Z 2018-09-18T12:16:59Z 2018-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion https://www.sciencedirect.com/science/article/pii/S1877959X18302152?via%3Dihub http://hdl.handle.net/20.500.12123/3381 1877-959X 1877-9603 https://doi.org/10.1016/j.ttbdis.2018.08.001 eng info:eu-repo/semantics/embargoedAccess application/pdf Ticks and Tick-borne Diseases 9 (6) : 1573-1585 (September 2018)
spellingShingle Rhipicephalus Sanguineus
Metastigmata
Identificación
Anatomía Animal
Secuencia Nucleotídica
Identification
Animal Morphology
Nucleotide Sequence
Garrapatas
Nava, Santiago
Beati, Lorenza
Venzal, José Manuel
Labruna, Marcelo B.
Szabó, Matías P.J.
Petney, Trevor
Saracho Bottero, Maria Noelia
Tarragona, Evelina Luisa
Dantas-Torres, Filipe
Silva, Maria M. Santos
Mangold, Atilio Jose
Guglielmone, Alberto
Estrada-Peña, Agustín
Rhipicephalus sanguineus (Latreille, 1806): Neotype designation, morphological re-description of all parasitic stages and molecular characterization
title Rhipicephalus sanguineus (Latreille, 1806): Neotype designation, morphological re-description of all parasitic stages and molecular characterization
title_full Rhipicephalus sanguineus (Latreille, 1806): Neotype designation, morphological re-description of all parasitic stages and molecular characterization
title_fullStr Rhipicephalus sanguineus (Latreille, 1806): Neotype designation, morphological re-description of all parasitic stages and molecular characterization
title_full_unstemmed Rhipicephalus sanguineus (Latreille, 1806): Neotype designation, morphological re-description of all parasitic stages and molecular characterization
title_short Rhipicephalus sanguineus (Latreille, 1806): Neotype designation, morphological re-description of all parasitic stages and molecular characterization
title_sort rhipicephalus sanguineus latreille 1806 neotype designation morphological re description of all parasitic stages and molecular characterization
topic Rhipicephalus Sanguineus
Metastigmata
Identificación
Anatomía Animal
Secuencia Nucleotídica
Identification
Animal Morphology
Nucleotide Sequence
Garrapatas
url https://www.sciencedirect.com/science/article/pii/S1877959X18302152?via%3Dihub
http://hdl.handle.net/20.500.12123/3381
https://doi.org/10.1016/j.ttbdis.2018.08.001
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