Carotenoids gene markers for sweetpotato (Ipomoea batatas L. Lam): applications in genetic mapping, diversity evaluation and cross‑species transference
Carotenoids play essential biological roles in plants, and genes involved in the carotenoid biosynthesis pathway are evolutionarily conserved. Orange sweetpotato is an important source of β-carotene, a precursor of vitamin A. In spite of this, only a few research studies have focussed on the molecul...
| Autores principales: | , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Springer
2018
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/3377 https://link.springer.com/article/10.1007%2Fs00438-013-0803-3#citeas https://doi.org/10.1007/s00438-013-0803-3 |
| _version_ | 1855035134325030912 |
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| author | Arizio, Carla Marcela Costa Tártara, Sabrina María Manifesto, Maria Marcela |
| author_browse | Arizio, Carla Marcela Costa Tártara, Sabrina María Manifesto, Maria Marcela |
| author_facet | Arizio, Carla Marcela Costa Tártara, Sabrina María Manifesto, Maria Marcela |
| author_sort | Arizio, Carla Marcela |
| collection | INTA Digital |
| description | Carotenoids play essential biological roles in plants, and genes involved in the carotenoid biosynthesis pathway are evolutionarily conserved. Orange sweetpotato is an important source of β-carotene, a precursor of vitamin A. In spite of this, only a few research studies have focussed on the molecular aspects of carotenoid genes regarding their specific sequence and structure. In this study, we used published carotenoid gene sequences from Ipomoea and other species for “exon-primed intron-crossing” approaches. Fifteen pairs of primers representing six carotenoid genes were designed for different introns, eleven of which amplified scorable and reproducible alleles. The sequence of PCR products showed high homology to the original ones. Moreover, the structure and sequence of the introns and exons from five carotenoid structural genes were partially defined. Intron length polymorphism and intron single nucleotide polymorphisms were detected in amplified sequences. Marker dosages and allelic segregations were analysed in a mapping population. The developed markers were evaluated in a set of Ipomoeas batatas accessions so as to analyse genetic diversity and conservation applicability. Using CG strategy combined with EPIC-PCR technique, we developed carotenoid gene markers in sweetpotato. We reported the first set of polymorphic Candidate Gene markers for I. batatas, and demonstrated transferability in seven wild Ipomoea species. We described the sequence and structure of carotenoid genes and introduced new information about genomic constitution and allele dosage. |
| format | info:ar-repo/semantics/artículo |
| id | INTA3377 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA33772018-09-17T17:46:49Z Carotenoids gene markers for sweetpotato (Ipomoea batatas L. Lam): applications in genetic mapping, diversity evaluation and cross‑species transference Arizio, Carla Marcela Costa Tártara, Sabrina María Manifesto, Maria Marcela Ipomoea Batatas Marcadores Genéticos Carotenoides Poliploidia Mapas Genéticos Genetic Markers Polyploidy Genetic Maps Carotenoids Camote (Planta) Transferability Carotenoids play essential biological roles in plants, and genes involved in the carotenoid biosynthesis pathway are evolutionarily conserved. Orange sweetpotato is an important source of β-carotene, a precursor of vitamin A. In spite of this, only a few research studies have focussed on the molecular aspects of carotenoid genes regarding their specific sequence and structure. In this study, we used published carotenoid gene sequences from Ipomoea and other species for “exon-primed intron-crossing” approaches. Fifteen pairs of primers representing six carotenoid genes were designed for different introns, eleven of which amplified scorable and reproducible alleles. The sequence of PCR products showed high homology to the original ones. Moreover, the structure and sequence of the introns and exons from five carotenoid structural genes were partially defined. Intron length polymorphism and intron single nucleotide polymorphisms were detected in amplified sequences. Marker dosages and allelic segregations were analysed in a mapping population. The developed markers were evaluated in a set of Ipomoeas batatas accessions so as to analyse genetic diversity and conservation applicability. Using CG strategy combined with EPIC-PCR technique, we developed carotenoid gene markers in sweetpotato. We reported the first set of polymorphic Candidate Gene markers for I. batatas, and demonstrated transferability in seven wild Ipomoea species. We described the sequence and structure of carotenoid genes and introduced new information about genomic constitution and allele dosage. Instituto de Recursos Biológicos Fil: Arizio, Carla Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina Fil: Costa Tártara, Sabrina María. Universidad Nacional de Luján; Argentina Fil: Manifesto, Maria Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina 2018-09-17T17:40:39Z 2018-09-17T17:40:39Z 2014-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/3377 https://link.springer.com/article/10.1007%2Fs00438-013-0803-3#citeas 1617-4615 1617-4623 (Online) https://doi.org/10.1007/s00438-013-0803-3 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Molecular genetics and genomics 289 (2) : 237–251. (April 2014) |
| spellingShingle | Ipomoea Batatas Marcadores Genéticos Carotenoides Poliploidia Mapas Genéticos Genetic Markers Polyploidy Genetic Maps Carotenoids Camote (Planta) Transferability Arizio, Carla Marcela Costa Tártara, Sabrina María Manifesto, Maria Marcela Carotenoids gene markers for sweetpotato (Ipomoea batatas L. Lam): applications in genetic mapping, diversity evaluation and cross‑species transference |
| title | Carotenoids gene markers for sweetpotato (Ipomoea batatas L. Lam): applications in genetic mapping, diversity evaluation and cross‑species transference |
| title_full | Carotenoids gene markers for sweetpotato (Ipomoea batatas L. Lam): applications in genetic mapping, diversity evaluation and cross‑species transference |
| title_fullStr | Carotenoids gene markers for sweetpotato (Ipomoea batatas L. Lam): applications in genetic mapping, diversity evaluation and cross‑species transference |
| title_full_unstemmed | Carotenoids gene markers for sweetpotato (Ipomoea batatas L. Lam): applications in genetic mapping, diversity evaluation and cross‑species transference |
| title_short | Carotenoids gene markers for sweetpotato (Ipomoea batatas L. Lam): applications in genetic mapping, diversity evaluation and cross‑species transference |
| title_sort | carotenoids gene markers for sweetpotato ipomoea batatas l lam applications in genetic mapping diversity evaluation and cross species transference |
| topic | Ipomoea Batatas Marcadores Genéticos Carotenoides Poliploidia Mapas Genéticos Genetic Markers Polyploidy Genetic Maps Carotenoids Camote (Planta) Transferability |
| url | http://hdl.handle.net/20.500.12123/3377 https://link.springer.com/article/10.1007%2Fs00438-013-0803-3#citeas https://doi.org/10.1007/s00438-013-0803-3 |
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