Molecular, morphological and pathogenic diversity of Sclerotinia sclerotiorum isolates from common bean (Phaseolus vulgaris) fields in Argentina
White mould, caused by Sclerotinia sclerotiorum, is one of the most threatening fungal diseases occurring across major bean production regions worldwide. In Argentina, under favourable weather conditions, up to 100% seed yield losses occur on susceptible common bean cultivars. The aim of this study...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
| Published: |
2018
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/3309 https://onlinelibrary.wiley.com/doi/abs/10.1111/ppa.12880 https://doi.org/10.1111/ppa.12880 |
| _version_ | 1855035121720098816 |
|---|---|
| author | Aban, Carla Luciana Taboada, Gisel María Spedaletti, Yamila Andrea Aparicio, Maria Cecilia Curti, Ramiro Nestor Casalderrey, Norma Beatriz Maggio, Maria Elisa Chocobar, Mario Osvaldo Salgado, M. Galvan, Marta Zulema |
| author_browse | Aban, Carla Luciana Aparicio, Maria Cecilia Casalderrey, Norma Beatriz Chocobar, Mario Osvaldo Curti, Ramiro Nestor Galvan, Marta Zulema Maggio, Maria Elisa Salgado, M. Spedaletti, Yamila Andrea Taboada, Gisel María |
| author_facet | Aban, Carla Luciana Taboada, Gisel María Spedaletti, Yamila Andrea Aparicio, Maria Cecilia Curti, Ramiro Nestor Casalderrey, Norma Beatriz Maggio, Maria Elisa Chocobar, Mario Osvaldo Salgado, M. Galvan, Marta Zulema |
| author_sort | Aban, Carla Luciana |
| collection | INTA Digital |
| description | White mould, caused by Sclerotinia sclerotiorum, is one of the most threatening fungal diseases occurring across major bean production regions worldwide. In Argentina, under favourable weather conditions, up to 100% seed yield losses occur on susceptible common bean cultivars. The aim of this study was to characterize the diversity of S. sclerotiorum isolates from six dry bean fields in the main production area of Argentina by means of molecular, morphological (mycelium colour, number and pattern of sclerotia distribution) and pathogenic approaches. Among 116 isolates analysed, high genotypic and morphological variability was observed. A total of 52 mycelial compatibility groups (MCGs) and 59 URPs (universal rice primers) molecular haplotypes were found. All the MCGs were location specific, while only 12% of the URP haplotypes were shared among locations. The molecular analysis of variance revealed a significant differentiation among populations, with higher genetic variability within the populations analysed than among them. The aggressiveness of the isolates towards bean seedlings was assessed in the greenhouse. Most of the isolates were highly aggressive, while no variation among locations was observed. The information generated in the present study provides, for the first time, information on the variability of S. sclerotiorum associated with white mould in the main common bean production area in Argentina. In addition, the findings suggest the occurrence of both clonal and sexual reproduction in the populations analysed. This work contributes to the development of sustainable manageme |
| format | info:ar-repo/semantics/artículo |
| id | INTA3309 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA33092025-02-12T14:46:17Z Molecular, morphological and pathogenic diversity of Sclerotinia sclerotiorum isolates from common bean (Phaseolus vulgaris) fields in Argentina Aban, Carla Luciana Taboada, Gisel María Spedaletti, Yamila Andrea Aparicio, Maria Cecilia Curti, Ramiro Nestor Casalderrey, Norma Beatriz Maggio, Maria Elisa Chocobar, Mario Osvaldo Salgado, M. Galvan, Marta Zulema Phaseolus vulgaris Fríjol (phaseolus) Sclerotinia sclerotiorum Enfermedades de las Plantas Enfermedades Fungosas Marcadores Genéticos Kidney Beans Plant Diseases Fungal Diseases Genetic Markers Moulds Moho Poroto Marcadores Moleculares Argentina White mould, caused by Sclerotinia sclerotiorum, is one of the most threatening fungal diseases occurring across major bean production regions worldwide. In Argentina, under favourable weather conditions, up to 100% seed yield losses occur on susceptible common bean cultivars. The aim of this study was to characterize the diversity of S. sclerotiorum isolates from six dry bean fields in the main production area of Argentina by means of molecular, morphological (mycelium colour, number and pattern of sclerotia distribution) and pathogenic approaches. Among 116 isolates analysed, high genotypic and morphological variability was observed. A total of 52 mycelial compatibility groups (MCGs) and 59 URPs (universal rice primers) molecular haplotypes were found. All the MCGs were location specific, while only 12% of the URP haplotypes were shared among locations. The molecular analysis of variance revealed a significant differentiation among populations, with higher genetic variability within the populations analysed than among them. The aggressiveness of the isolates towards bean seedlings was assessed in the greenhouse. Most of the isolates were highly aggressive, while no variation among locations was observed. The information generated in the present study provides, for the first time, information on the variability of S. sclerotiorum associated with white mould in the main common bean production area in Argentina. In addition, the findings suggest the occurrence of both clonal and sexual reproduction in the populations analysed. This work contributes to the development of sustainable manageme EEA Salta Fil: Aban, Carla Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Taboada, Gisel María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Spedaletti, Yamila Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Aparicio, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Curti, Ramiro Nestor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina Fil: Casalderrey, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Maggio, Maria Elisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Chocobar, Mario Osvaldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Salgado, M. Actividad Privada; Argentina Fil: Galvan, Marta Zulema. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina 2018-09-07T14:14:12Z 2018-09-07T14:14:12Z 2018 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/3309 https://onlinelibrary.wiley.com/doi/abs/10.1111/ppa.12880 0032-0862 1365-3059 https://doi.org/10.1111/ppa.12880 eng info:eu-repo/semantics/restrictedAccess application/pdf Plant Pathology 67 (8) : 1740-1748 (October 2018) |
| spellingShingle | Phaseolus vulgaris Fríjol (phaseolus) Sclerotinia sclerotiorum Enfermedades de las Plantas Enfermedades Fungosas Marcadores Genéticos Kidney Beans Plant Diseases Fungal Diseases Genetic Markers Moulds Moho Poroto Marcadores Moleculares Argentina Aban, Carla Luciana Taboada, Gisel María Spedaletti, Yamila Andrea Aparicio, Maria Cecilia Curti, Ramiro Nestor Casalderrey, Norma Beatriz Maggio, Maria Elisa Chocobar, Mario Osvaldo Salgado, M. Galvan, Marta Zulema Molecular, morphological and pathogenic diversity of Sclerotinia sclerotiorum isolates from common bean (Phaseolus vulgaris) fields in Argentina |
| title | Molecular, morphological and pathogenic diversity of Sclerotinia sclerotiorum isolates from common bean (Phaseolus vulgaris) fields in Argentina |
| title_full | Molecular, morphological and pathogenic diversity of Sclerotinia sclerotiorum isolates from common bean (Phaseolus vulgaris) fields in Argentina |
| title_fullStr | Molecular, morphological and pathogenic diversity of Sclerotinia sclerotiorum isolates from common bean (Phaseolus vulgaris) fields in Argentina |
| title_full_unstemmed | Molecular, morphological and pathogenic diversity of Sclerotinia sclerotiorum isolates from common bean (Phaseolus vulgaris) fields in Argentina |
| title_short | Molecular, morphological and pathogenic diversity of Sclerotinia sclerotiorum isolates from common bean (Phaseolus vulgaris) fields in Argentina |
| title_sort | molecular morphological and pathogenic diversity of sclerotinia sclerotiorum isolates from common bean phaseolus vulgaris fields in argentina |
| topic | Phaseolus vulgaris Fríjol (phaseolus) Sclerotinia sclerotiorum Enfermedades de las Plantas Enfermedades Fungosas Marcadores Genéticos Kidney Beans Plant Diseases Fungal Diseases Genetic Markers Moulds Moho Poroto Marcadores Moleculares Argentina |
| url | http://hdl.handle.net/20.500.12123/3309 https://onlinelibrary.wiley.com/doi/abs/10.1111/ppa.12880 https://doi.org/10.1111/ppa.12880 |
| work_keys_str_mv | AT abancarlaluciana molecularmorphologicalandpathogenicdiversityofsclerotiniasclerotiorumisolatesfromcommonbeanphaseolusvulgarisfieldsinargentina AT taboadagiselmaria molecularmorphologicalandpathogenicdiversityofsclerotiniasclerotiorumisolatesfromcommonbeanphaseolusvulgarisfieldsinargentina AT spedalettiyamilaandrea molecularmorphologicalandpathogenicdiversityofsclerotiniasclerotiorumisolatesfromcommonbeanphaseolusvulgarisfieldsinargentina AT apariciomariacecilia molecularmorphologicalandpathogenicdiversityofsclerotiniasclerotiorumisolatesfromcommonbeanphaseolusvulgarisfieldsinargentina AT curtiramironestor molecularmorphologicalandpathogenicdiversityofsclerotiniasclerotiorumisolatesfromcommonbeanphaseolusvulgarisfieldsinargentina AT casalderreynormabeatriz molecularmorphologicalandpathogenicdiversityofsclerotiniasclerotiorumisolatesfromcommonbeanphaseolusvulgarisfieldsinargentina AT maggiomariaelisa molecularmorphologicalandpathogenicdiversityofsclerotiniasclerotiorumisolatesfromcommonbeanphaseolusvulgarisfieldsinargentina AT chocobarmarioosvaldo molecularmorphologicalandpathogenicdiversityofsclerotiniasclerotiorumisolatesfromcommonbeanphaseolusvulgarisfieldsinargentina AT salgadom molecularmorphologicalandpathogenicdiversityofsclerotiniasclerotiorumisolatesfromcommonbeanphaseolusvulgarisfieldsinargentina AT galvanmartazulema molecularmorphologicalandpathogenicdiversityofsclerotiniasclerotiorumisolatesfromcommonbeanphaseolusvulgarisfieldsinargentina |