Study of the effects of spray drying in whey‐starch on the probiotic capacity of Lactobacillus rhamnosus 64 in the gut of mice
Aims: To evaluate the effects of spray drying of Lactobacillus rhamnosus 64 on its capacity to modulate the gut immune response and on the attenuation of TNBS‐induced colitis in mice. Methods and Results: Lactobacillus rhamnosus 64 was spray dried in cheese whey‐starch solution and administered to...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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2018
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| Online Access: | https://onlinelibrary.wiley.com/doi/abs/10.1111/jam.13567 http://hdl.handle.net/20.500.12123/3291 https://doi.org/10.1111/jam.13567 |
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| author | Lavari, Luisina Burns, Patricia Graciela Paez, Roxana Beatriz Reinheimer, Jorge Alberto Vinderola, Celso Gabriel |
| author_browse | Burns, Patricia Graciela Lavari, Luisina Paez, Roxana Beatriz Reinheimer, Jorge Alberto Vinderola, Celso Gabriel |
| author_facet | Lavari, Luisina Burns, Patricia Graciela Paez, Roxana Beatriz Reinheimer, Jorge Alberto Vinderola, Celso Gabriel |
| author_sort | Lavari, Luisina |
| collection | INTA Digital |
| description | Aims: To evaluate the effects of spray drying of Lactobacillus rhamnosus 64 on its capacity to modulate the gut immune response and on the attenuation of TNBS‐induced colitis in mice.
Methods and Results: Lactobacillus rhamnosus 64 was spray dried in cheese whey‐starch solution and administered to mice for 3, 6 or 10 consecutive days. Peritoneal macrophage phagocytic activity, secretory IgA levels in the small intestinal fluid and TNFα, IFNγ, IL‐10, IL‐6 and IL‐2 levels in homogenates of the small and large intestine were determined. The effects of spray drying were also evaluated in an acute model of Trinitrobenzenesulfonic acid (TNBS)‐induced colitis. A shift in the regulation of immune parameters, particularly the cytokine profile, was observed for mice treated with the spray‐dried culture, compared to the profile observed in animals that received the strain as fresh culture (FC). The spray‐dried culture of L. rhamnosus 64 showed anti‐inflammatory properties in murine model of TNBS‐induced colitis.
Conclusions: The spray‐drying process of L. rhamnosus 64 in whey‐starch modified its immunomodulating capacity in healthy animals and conferred enhanced protection in an in vivo model of inflammation. |
| format | info:ar-repo/semantics/artículo |
| id | INTA3291 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA32912018-09-12T15:00:00Z Study of the effects of spray drying in whey‐starch on the probiotic capacity of Lactobacillus rhamnosus 64 in the gut of mice Lavari, Luisina Burns, Patricia Graciela Paez, Roxana Beatriz Reinheimer, Jorge Alberto Vinderola, Celso Gabriel Lactobacillus Probióticos Lactosuero Almidón Ratón Probiotics Whey Starch Mice Spray Drying Secado por Pulverización Lactobacillus rhamnosus Aims: To evaluate the effects of spray drying of Lactobacillus rhamnosus 64 on its capacity to modulate the gut immune response and on the attenuation of TNBS‐induced colitis in mice. Methods and Results: Lactobacillus rhamnosus 64 was spray dried in cheese whey‐starch solution and administered to mice for 3, 6 or 10 consecutive days. Peritoneal macrophage phagocytic activity, secretory IgA levels in the small intestinal fluid and TNFα, IFNγ, IL‐10, IL‐6 and IL‐2 levels in homogenates of the small and large intestine were determined. The effects of spray drying were also evaluated in an acute model of Trinitrobenzenesulfonic acid (TNBS)‐induced colitis. A shift in the regulation of immune parameters, particularly the cytokine profile, was observed for mice treated with the spray‐dried culture, compared to the profile observed in animals that received the strain as fresh culture (FC). The spray‐dried culture of L. rhamnosus 64 showed anti‐inflammatory properties in murine model of TNBS‐induced colitis. Conclusions: The spray‐drying process of L. rhamnosus 64 in whey‐starch modified its immunomodulating capacity in healthy animals and conferred enhanced protection in an in vivo model of inflammation. EEA Rafaela Fil: Lavari, Luisina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Burns, Patricia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactologia Industrial; Argentina Fil: Paez, Roxana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactologia Industrial; Argentina Fil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactologia Industrial; Argentina 2018-09-04T15:58:40Z 2018-09-04T15:58:40Z 2017-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://onlinelibrary.wiley.com/doi/abs/10.1111/jam.13567 http://hdl.handle.net/20.500.12123/3291 1364-5072 1365-2672 https://doi.org/10.1111/jam.13567 eng info:eu-repo/semantics/restrictedAccess application/pdf Journal of applied microbiology 123 (4) : 992-1002. (October 2017) |
| spellingShingle | Lactobacillus Probióticos Lactosuero Almidón Ratón Probiotics Whey Starch Mice Spray Drying Secado por Pulverización Lactobacillus rhamnosus Lavari, Luisina Burns, Patricia Graciela Paez, Roxana Beatriz Reinheimer, Jorge Alberto Vinderola, Celso Gabriel Study of the effects of spray drying in whey‐starch on the probiotic capacity of Lactobacillus rhamnosus 64 in the gut of mice |
| title | Study of the effects of spray drying in whey‐starch on the probiotic capacity of Lactobacillus rhamnosus 64 in the gut of mice |
| title_full | Study of the effects of spray drying in whey‐starch on the probiotic capacity of Lactobacillus rhamnosus 64 in the gut of mice |
| title_fullStr | Study of the effects of spray drying in whey‐starch on the probiotic capacity of Lactobacillus rhamnosus 64 in the gut of mice |
| title_full_unstemmed | Study of the effects of spray drying in whey‐starch on the probiotic capacity of Lactobacillus rhamnosus 64 in the gut of mice |
| title_short | Study of the effects of spray drying in whey‐starch on the probiotic capacity of Lactobacillus rhamnosus 64 in the gut of mice |
| title_sort | study of the effects of spray drying in whey starch on the probiotic capacity of lactobacillus rhamnosus 64 in the gut of mice |
| topic | Lactobacillus Probióticos Lactosuero Almidón Ratón Probiotics Whey Starch Mice Spray Drying Secado por Pulverización Lactobacillus rhamnosus |
| url | https://onlinelibrary.wiley.com/doi/abs/10.1111/jam.13567 http://hdl.handle.net/20.500.12123/3291 https://doi.org/10.1111/jam.13567 |
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