Molecular mapping of vernalization requirement and fertility restoration genes in carrot
Carrot (Daucus carota L.) is a cool-season vegetable normally classified as a biennial species, requiring vernalization to induce flowering. Nevertheless, some cultivars adapted to warmer climates require less vernalization and can be classified as annual. Most modern carrot cultivars are hybrids wh...
| Main Authors: | , , , |
|---|---|
| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
| Published: |
2018
|
| Subjects: | |
| Online Access: | https://link.springer.com/article/10.1007/s00122-012-1989-1 http://hdl.handle.net/20.500.12123/3253 https://doi.org/10.1007/s00122-012-1989-1 |
| _version_ | 1855035111123189760 |
|---|---|
| author | Alessandro, Maria Soledad Galmarini, Claudio Romulo Iorizzo, Massimo Simon, Philipp W. |
| author_browse | Alessandro, Maria Soledad Galmarini, Claudio Romulo Iorizzo, Massimo Simon, Philipp W. |
| author_facet | Alessandro, Maria Soledad Galmarini, Claudio Romulo Iorizzo, Massimo Simon, Philipp W. |
| author_sort | Alessandro, Maria Soledad |
| collection | INTA Digital |
| description | Carrot (Daucus carota L.) is a cool-season vegetable normally classified as a biennial species, requiring vernalization to induce flowering. Nevertheless, some cultivars adapted to warmer climates require less vernalization and can be classified as annual. Most modern carrot cultivars are hybrids which rely upon cytoplasmic male-sterility for commercial production. One major gene controlling floral initiation and several genes restoring male fertility have been reported but none have been mapped. The objective of the present work was to develop the first linkage map of carrot locating the genomic regions that control vernalization response and fertility restoration. Using an F2 progeny, derived from the intercross between the annual cultivar ‘Criolla INTA’ and a petaloid male sterile biennial carrot evaluated over 2 years, both early flowering habit, which we name Vrn1, and restoration of petaloid cytoplasmic male sterility, which we name Rf1, were found to be dominant traits conditioned by single genes. On a map of 355 markers covering all 9 chromosomes with a total map length of 669 cM and an average marker-to-marker distance of 1.88 cM, Vrn1 mapped to chromosome 2 with flanking markers at 0.70 and 0.46 cM, and Rf1 mapped to chromosome 9 with flanking markers at 4.38 and 1.12 cM. These are the first two reproductive traits mapped in the carrot genome, and their map location and flanking markers provide valuable tools for studying traits important for carrot domestication and reproductive biology, as well as facilitating carrot breeding. |
| format | info:ar-repo/semantics/artículo |
| id | INTA3253 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA32532018-08-30T14:12:22Z Molecular mapping of vernalization requirement and fertility restoration genes in carrot Alessandro, Maria Soledad Galmarini, Claudio Romulo Iorizzo, Massimo Simon, Philipp W. Zanahoria Daucus Carota Marcadores Genéticos Vernalización Genética Polimorfismo de Longitud de Fragmentos Amplificados Carrots Genetic Markers Vernalization Genetics Amplified Fragment Length Polymorphism Marcadores Moleculares Carrot (Daucus carota L.) is a cool-season vegetable normally classified as a biennial species, requiring vernalization to induce flowering. Nevertheless, some cultivars adapted to warmer climates require less vernalization and can be classified as annual. Most modern carrot cultivars are hybrids which rely upon cytoplasmic male-sterility for commercial production. One major gene controlling floral initiation and several genes restoring male fertility have been reported but none have been mapped. The objective of the present work was to develop the first linkage map of carrot locating the genomic regions that control vernalization response and fertility restoration. Using an F2 progeny, derived from the intercross between the annual cultivar ‘Criolla INTA’ and a petaloid male sterile biennial carrot evaluated over 2 years, both early flowering habit, which we name Vrn1, and restoration of petaloid cytoplasmic male sterility, which we name Rf1, were found to be dominant traits conditioned by single genes. On a map of 355 markers covering all 9 chromosomes with a total map length of 669 cM and an average marker-to-marker distance of 1.88 cM, Vrn1 mapped to chromosome 2 with flanking markers at 0.70 and 0.46 cM, and Rf1 mapped to chromosome 9 with flanking markers at 4.38 and 1.12 cM. These are the first two reproductive traits mapped in the carrot genome, and their map location and flanking markers provide valuable tools for studying traits important for carrot domestication and reproductive biology, as well as facilitating carrot breeding. EEA La Consulta Fil: Alessandro, Maria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina Fil: Galmarini, Claudio Romulo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina Fil: Iorizzo, Massimo. University of Wisconsin. Department of Horticulture; Estados Unidos. Fil: Simon, Philipp W. United States Department of Agriculture–Agricultural Research Service. Vegetable Crops Research Unit; Estados Unidos 2018-08-30T14:10:57Z 2018-08-30T14:10:57Z 2013-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007/s00122-012-1989-1 http://hdl.handle.net/20.500.12123/3253 0040-5752 1432-2242 https://doi.org/10.1007/s00122-012-1989-1 eng info:eu-repo/semantics/restrictedAccess application/pdf Theoretical and Applied Genetics 126 (2) : 415–423 (February 2013) |
| spellingShingle | Zanahoria Daucus Carota Marcadores Genéticos Vernalización Genética Polimorfismo de Longitud de Fragmentos Amplificados Carrots Genetic Markers Vernalization Genetics Amplified Fragment Length Polymorphism Marcadores Moleculares Alessandro, Maria Soledad Galmarini, Claudio Romulo Iorizzo, Massimo Simon, Philipp W. Molecular mapping of vernalization requirement and fertility restoration genes in carrot |
| title | Molecular mapping of vernalization requirement and fertility restoration genes in carrot |
| title_full | Molecular mapping of vernalization requirement and fertility restoration genes in carrot |
| title_fullStr | Molecular mapping of vernalization requirement and fertility restoration genes in carrot |
| title_full_unstemmed | Molecular mapping of vernalization requirement and fertility restoration genes in carrot |
| title_short | Molecular mapping of vernalization requirement and fertility restoration genes in carrot |
| title_sort | molecular mapping of vernalization requirement and fertility restoration genes in carrot |
| topic | Zanahoria Daucus Carota Marcadores Genéticos Vernalización Genética Polimorfismo de Longitud de Fragmentos Amplificados Carrots Genetic Markers Vernalization Genetics Amplified Fragment Length Polymorphism Marcadores Moleculares |
| url | https://link.springer.com/article/10.1007/s00122-012-1989-1 http://hdl.handle.net/20.500.12123/3253 https://doi.org/10.1007/s00122-012-1989-1 |
| work_keys_str_mv | AT alessandromariasoledad molecularmappingofvernalizationrequirementandfertilityrestorationgenesincarrot AT galmariniclaudioromulo molecularmappingofvernalizationrequirementandfertilityrestorationgenesincarrot AT iorizzomassimo molecularmappingofvernalizationrequirementandfertilityrestorationgenesincarrot AT simonphilippw molecularmappingofvernalizationrequirementandfertilityrestorationgenesincarrot |