Expression profile of transcripts encoding cell wall remodelling proteins in tomato fruit cv. Micro-Tom subjected to 15°C storage
To extend fruit market life, tomatoes are harvested before red ripe and kept at temperatures below optimum (20°C). In this work, Micro-Tom tomatoes stored at 20°C (normal ripening) were compared with those stored at 15°C or 4°C (chilling injury inducer) for 7 days. In contrast to 4°C, storage at 15°...
| Autores principales: | , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Acceso en línea: | http://www.publish.csiro.au/FP/FP12272 http://hdl.handle.net/20.500.12123/3213 https://doi.org/10.1071/FP12272 |
| _version_ | 1855035103612239872 |
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| author | Müller, Gabriela L. Budde, Claudio Olaf Lauxmann, Martín Alexander Triassi, Agustina Andreo, Carlos Santiago Drincovich, María Fabiana Lara, María Valeria |
| author_browse | Andreo, Carlos Santiago Budde, Claudio Olaf Drincovich, María Fabiana Lara, María Valeria Lauxmann, Martín Alexander Müller, Gabriela L. Triassi, Agustina |
| author_facet | Müller, Gabriela L. Budde, Claudio Olaf Lauxmann, Martín Alexander Triassi, Agustina Andreo, Carlos Santiago Drincovich, María Fabiana Lara, María Valeria |
| author_sort | Müller, Gabriela L. |
| collection | INTA Digital |
| description | To extend fruit market life, tomatoes are harvested before red ripe and kept at temperatures below optimum (20°C). In this work, Micro-Tom tomatoes stored at 20°C (normal ripening) were compared with those stored at 15°C or 4°C (chilling injury inducer) for 7 days. In contrast to 4°C, storage at 15°C delayed ripening with the benefit of not enhancing oxidative metabolism and of enabling ripening upon being transferred to 20°C. The transcriptional expression profile of enzymes related to cell wall metabolism was compared at the three temperatures. Although endo-β-1,4-glucanase (Cel1), which is associated with fruit decay, was largely increased after removal from 4°C storage, its expression was not modified in fruits stored at 15°C. Enhanced transcriptional expression of xyloglucan endotransgylcosylase/hydrolases (XTHs) XTH1, –2, –10 and –11, and of two β-xylosidases (Xyl1–2) was detected in fruits stored at 15°C with respect to those at 20°C. Following 2 days at 20°C, these transcripts remained higher in fruits stored at 15°C and XHT3 and –9 also increased. Ethylene evolution was similar in fruits kept at 15°C and 20°C; thus, the changes in the transcript profile and fruit properties between these treatments may be under the control of factors other than ethylene. |
| format | info:ar-repo/semantics/artículo |
| id | INTA3213 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA32132019-06-13T12:41:48Z Expression profile of transcripts encoding cell wall remodelling proteins in tomato fruit cv. Micro-Tom subjected to 15°C storage Müller, Gabriela L. Budde, Claudio Olaf Lauxmann, Martín Alexander Triassi, Agustina Andreo, Carlos Santiago Drincovich, María Fabiana Lara, María Valeria Tomate Solanum Lycopersicum Fisiología Postcosecha Maduración en Postcosecha Pared Celular Metabolismo Almacenamiento en Frío Aptitud para la Conservación Tomatoes Postharvest Physiology Postharvest Ripening Cell Walls Metabolism Cold Storage Keeping Quality Metabolismo de la Pared Celular Daño por Frío Endotransglicosilasa/Hidrolasa Xyloglucan β-xilosidasa Cell Wall Metabolism Chilling Injury Endotransgylcosylase/Hydrolase Xyloglucan β-xylosidase To extend fruit market life, tomatoes are harvested before red ripe and kept at temperatures below optimum (20°C). In this work, Micro-Tom tomatoes stored at 20°C (normal ripening) were compared with those stored at 15°C or 4°C (chilling injury inducer) for 7 days. In contrast to 4°C, storage at 15°C delayed ripening with the benefit of not enhancing oxidative metabolism and of enabling ripening upon being transferred to 20°C. The transcriptional expression profile of enzymes related to cell wall metabolism was compared at the three temperatures. Although endo-β-1,4-glucanase (Cel1), which is associated with fruit decay, was largely increased after removal from 4°C storage, its expression was not modified in fruits stored at 15°C. Enhanced transcriptional expression of xyloglucan endotransgylcosylase/hydrolases (XTHs) XTH1, –2, –10 and –11, and of two β-xylosidases (Xyl1–2) was detected in fruits stored at 15°C with respect to those at 20°C. Following 2 days at 20°C, these transcripts remained higher in fruits stored at 15°C and XHT3 and –9 also increased. Ethylene evolution was similar in fruits kept at 15°C and 20°C; thus, the changes in the transcript profile and fruit properties between these treatments may be under the control of factors other than ethylene. EEA San Pedro Fil: Müller, Gabriela L. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina. Fil: Budde, Claudio Olaf. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina. Fil: Lauxmann, Martin A. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina. Fil: Triassi, Agustina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina. Fil: Andreo, Carlos Santiago. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina. Fil: Drincovich, María Fabiana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina. Fil: Lara, María Valeria. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina. 2018-08-28T14:03:21Z 2018-08-28T14:03:21Z 2013 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://www.publish.csiro.au/FP/FP12272 http://hdl.handle.net/20.500.12123/3213 1445-4416 1445-4408 https://doi.org/10.1071/FP12272 eng info:eu-repo/semantics/restrictedAccess application/pdf Functional Plant Biology 40 (5) : 449-459. (2013) |
| spellingShingle | Tomate Solanum Lycopersicum Fisiología Postcosecha Maduración en Postcosecha Pared Celular Metabolismo Almacenamiento en Frío Aptitud para la Conservación Tomatoes Postharvest Physiology Postharvest Ripening Cell Walls Metabolism Cold Storage Keeping Quality Metabolismo de la Pared Celular Daño por Frío Endotransglicosilasa/Hidrolasa Xyloglucan β-xilosidasa Cell Wall Metabolism Chilling Injury Endotransgylcosylase/Hydrolase Xyloglucan β-xylosidase Müller, Gabriela L. Budde, Claudio Olaf Lauxmann, Martín Alexander Triassi, Agustina Andreo, Carlos Santiago Drincovich, María Fabiana Lara, María Valeria Expression profile of transcripts encoding cell wall remodelling proteins in tomato fruit cv. Micro-Tom subjected to 15°C storage |
| title | Expression profile of transcripts encoding cell wall remodelling proteins in tomato fruit cv. Micro-Tom subjected to 15°C storage |
| title_full | Expression profile of transcripts encoding cell wall remodelling proteins in tomato fruit cv. Micro-Tom subjected to 15°C storage |
| title_fullStr | Expression profile of transcripts encoding cell wall remodelling proteins in tomato fruit cv. Micro-Tom subjected to 15°C storage |
| title_full_unstemmed | Expression profile of transcripts encoding cell wall remodelling proteins in tomato fruit cv. Micro-Tom subjected to 15°C storage |
| title_short | Expression profile of transcripts encoding cell wall remodelling proteins in tomato fruit cv. Micro-Tom subjected to 15°C storage |
| title_sort | expression profile of transcripts encoding cell wall remodelling proteins in tomato fruit cv micro tom subjected to 15°c storage |
| topic | Tomate Solanum Lycopersicum Fisiología Postcosecha Maduración en Postcosecha Pared Celular Metabolismo Almacenamiento en Frío Aptitud para la Conservación Tomatoes Postharvest Physiology Postharvest Ripening Cell Walls Metabolism Cold Storage Keeping Quality Metabolismo de la Pared Celular Daño por Frío Endotransglicosilasa/Hidrolasa Xyloglucan β-xilosidasa Cell Wall Metabolism Chilling Injury Endotransgylcosylase/Hydrolase Xyloglucan β-xylosidase |
| url | http://www.publish.csiro.au/FP/FP12272 http://hdl.handle.net/20.500.12123/3213 https://doi.org/10.1071/FP12272 |
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