Biological activities of polyphenols-enriched propolis from Argentina arid regions

Background: Propolis is a bioactive natural product collected by honeybees (Apis mellifera) from plant sources. Purpose: This study was undertaken to determine the effect of propolis extracts from arid region of Argentina, on the activity/expression of pro-inflammatory enzymes, and as potential...

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Main Authors: Salas, Ana Lilia, Alberto, María Rosa, Zampini, Iris Catiana, Cuello, Ana Soledad, Maldonado, Luis Maria, Ríos, José Luis, Schmeda Hirschmann, Guillermo, Isla, María Inés
Format: info:ar-repo/semantics/artículo
Language:Inglés
Published: Elsevier 2018
Subjects:
Online Access:http://hdl.handle.net/20.500.12123/3195
https://www.sciencedirect.com/science/article/pii/S0944711315003591?via%3Dihub
https://doi.org/10.1016/j.phymed.2015.11.007
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author Salas, Ana Lilia
Alberto, María Rosa
Zampini, Iris Catiana
Cuello, Ana Soledad
Maldonado, Luis Maria
Ríos, José Luis
Schmeda Hirschmann, Guillermo
Isla, María Inés
author_browse Alberto, María Rosa
Cuello, Ana Soledad
Isla, María Inés
Maldonado, Luis Maria
Ríos, José Luis
Salas, Ana Lilia
Schmeda Hirschmann, Guillermo
Zampini, Iris Catiana
author_facet Salas, Ana Lilia
Alberto, María Rosa
Zampini, Iris Catiana
Cuello, Ana Soledad
Maldonado, Luis Maria
Ríos, José Luis
Schmeda Hirschmann, Guillermo
Isla, María Inés
author_sort Salas, Ana Lilia
collection INTA Digital
description Background: Propolis is a bioactive natural product collected by honeybees (Apis mellifera) from plant sources. Purpose: This study was undertaken to determine the effect of propolis extracts from arid region of Argentina, on the activity/expression of pro-inflammatory enzymes, and as potential free radical scavenger, antifungal and anthelmintic agent as well as to get a first insight into the polyphenolic profile of the active fractions. Study design/methods: Two propolis samples were collected in different time from hives located in Tucumán, Argentina. They are representative of the collection time of the raw material for phytotherapeutical purposes. Ethanolic extracts from both propolis were obtained. The PEEs were analyzed for total polyphenol (TP), non-flavonoid phenols (NFP) and flavonoid (FP) content followed by HPLC-DAD analysis and identification of components by HPLC-MS/MSn. The potentiality as anti-inflammatory (LOX, COX, iNOS enzymes), antioxidant, antifungal and nematicidal was determined. Results: PEEs contain high levels of TP, NFP and FP, including cinnamic acid, caffeic acid prenyl ester, caffeoyl dihydrocaffeate and caffeic acid 3,4-dihydroxyphenethyl ester, liquiritigenin, 2′,4′-dihydroxychalcone and 2′,4′-dihydroxy-3′-methoxychalcone. The PEEs in vitro reduced the activity of LOX and COX-2. Pretreatment of RAW 264.7 cells with PEEs before the induction of inflammatory state, inhibited NO overproduction and the iNOS protein expression was significantly decreased. The PEEs exhibited antioxidant, antifungal (Candida sp.) and nematicidal effect (C. elegans). Conclusion: These findings show the potential use of characterized PEEs from arid regions of Argentina as phytomedicine.
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spelling INTA31952022-08-12T16:10:49Z Biological activities of polyphenols-enriched propolis from Argentina arid regions Salas, Ana Lilia Alberto, María Rosa Zampini, Iris Catiana Cuello, Ana Soledad Maldonado, Luis Maria Ríos, José Luis Schmeda Hirschmann, Guillermo Isla, María Inés Propóleos Polifenoles Zona Arida Antihelmínticos Propiedades Antimicosicas Antinflamatorios Propolis Polyphenols Arid Zones Anthelmintics Antifungal Properties Antiinflammatory Agents Argentina Background: Propolis is a bioactive natural product collected by honeybees (Apis mellifera) from plant sources. Purpose: This study was undertaken to determine the effect of propolis extracts from arid region of Argentina, on the activity/expression of pro-inflammatory enzymes, and as potential free radical scavenger, antifungal and anthelmintic agent as well as to get a first insight into the polyphenolic profile of the active fractions. Study design/methods: Two propolis samples were collected in different time from hives located in Tucumán, Argentina. They are representative of the collection time of the raw material for phytotherapeutical purposes. Ethanolic extracts from both propolis were obtained. The PEEs were analyzed for total polyphenol (TP), non-flavonoid phenols (NFP) and flavonoid (FP) content followed by HPLC-DAD analysis and identification of components by HPLC-MS/MSn. The potentiality as anti-inflammatory (LOX, COX, iNOS enzymes), antioxidant, antifungal and nematicidal was determined. Results: PEEs contain high levels of TP, NFP and FP, including cinnamic acid, caffeic acid prenyl ester, caffeoyl dihydrocaffeate and caffeic acid 3,4-dihydroxyphenethyl ester, liquiritigenin, 2′,4′-dihydroxychalcone and 2′,4′-dihydroxy-3′-methoxychalcone. The PEEs in vitro reduced the activity of LOX and COX-2. Pretreatment of RAW 264.7 cells with PEEs before the induction of inflammatory state, inhibited NO overproduction and the iNOS protein expression was significantly decreased. The PEEs exhibited antioxidant, antifungal (Candida sp.) and nematicidal effect (C. elegans). Conclusion: These findings show the potential use of characterized PEEs from arid regions of Argentina as phytomedicine. EEA Famaillá Fil: Salas, Ana Lilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Laboratorio de Investigación de Productos Naturales. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste. Laboratorio de Investigación de Productos Naturales ; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina Fil: Alberto, Maria Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Laboratorio de Investigación de Productos Naturales. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste. Laboratorio de Investigación de Productos Naturales ; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina Fil: Zampini, Iris Catiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Laboratorio de Investigación de Productos Naturales. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste. Laboratorio de Investigación de Productos Naturales ; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina Fil: Cuello, Ana Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Laboratorio de Investigación de Productos Naturales. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste. Laboratorio de Investigación de Productos Naturales ; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina Fil: Maldonado, Luis Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina Fil: Ríos, José Luis. Universidad de Valencia. Facultad de Farmacia. Departamento de Farmacología; España Fil: Schmeda Hirschmann, Guillermo. Universidad de Talca. Instituto de Química de Recursos Naturales. Laboratorio de Química de Productos Naturales ; Chile Fil: Isla, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Laboratorio de Investigación de Productos Naturales. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste. Laboratorio de Investigación de Productos Naturales ; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina 2018-08-27T15:25:15Z 2018-08-27T15:25:15Z 2016-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/3195 https://www.sciencedirect.com/science/article/pii/S0944711315003591?via%3Dihub 0944-7113 https://doi.org/10.1016/j.phymed.2015.11.007 eng info:eu-repo/semantics/restrictedAccess application/pdf Argentina (nation) Elsevier Phytomedicine 23 (1) : 27-3. (January 2016)
spellingShingle Propóleos
Polifenoles
Zona Arida
Antihelmínticos
Propiedades Antimicosicas
Antinflamatorios
Propolis
Polyphenols
Arid Zones
Anthelmintics
Antifungal Properties
Antiinflammatory Agents
Argentina
Salas, Ana Lilia
Alberto, María Rosa
Zampini, Iris Catiana
Cuello, Ana Soledad
Maldonado, Luis Maria
Ríos, José Luis
Schmeda Hirschmann, Guillermo
Isla, María Inés
Biological activities of polyphenols-enriched propolis from Argentina arid regions
title Biological activities of polyphenols-enriched propolis from Argentina arid regions
title_full Biological activities of polyphenols-enriched propolis from Argentina arid regions
title_fullStr Biological activities of polyphenols-enriched propolis from Argentina arid regions
title_full_unstemmed Biological activities of polyphenols-enriched propolis from Argentina arid regions
title_short Biological activities of polyphenols-enriched propolis from Argentina arid regions
title_sort biological activities of polyphenols enriched propolis from argentina arid regions
topic Propóleos
Polifenoles
Zona Arida
Antihelmínticos
Propiedades Antimicosicas
Antinflamatorios
Propolis
Polyphenols
Arid Zones
Anthelmintics
Antifungal Properties
Antiinflammatory Agents
Argentina
url http://hdl.handle.net/20.500.12123/3195
https://www.sciencedirect.com/science/article/pii/S0944711315003591?via%3Dihub
https://doi.org/10.1016/j.phymed.2015.11.007
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