Evidence of Recombinant Isolates of Potato Virus Y (PVY) in Argentina
In order to make a first approach in the identification of the genetic diversity of Potato virus Y (PVY) in Argentina, 46 PVY isolates from different potato growing regions of Argentina were characterized both, biological and serologically. Five of them (ST11, RCA5a, RCA6, RCA14b and SSF6) were sele...
| Autores principales: | , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer Netherlands
2018
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| Materias: | |
| Acceso en línea: | https://link.springer.com/article/10.1007/s12230-016-9564-y http://hdl.handle.net/20.500.12123/3163 https://doi.org/10.1007/s12230-016-9564-y |
| _version_ | 1855035094966730752 |
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| author | Colavita, Monica Lancia, Marcos Feingold, Sergio Enrique Massa, Gabriela Alejandra |
| author_browse | Colavita, Monica Feingold, Sergio Enrique Lancia, Marcos Massa, Gabriela Alejandra |
| author_facet | Colavita, Monica Lancia, Marcos Feingold, Sergio Enrique Massa, Gabriela Alejandra |
| author_sort | Colavita, Monica |
| collection | INTA Digital |
| description | In order to make a first approach in the identification of the genetic diversity of Potato virus Y (PVY) in Argentina, 46 PVY isolates from different potato growing regions of Argentina were characterized both, biological and serologically. Five of them (ST11, RCA5a, RCA6, RCA14b and SSF6) were selected for further genomic analyses. Four genomic fragments containing hot spot regions of recombination (HSR) reported previously were sequenced in each isolate and compared to PVYN (CS434575.1) and PVYO (U09509.1) reference genomes looking for genomic recombinations. Isolates with one, two or three recombination points were identified among these, including the two strains considered typical PVYN (RCA5b) and PVYO (SSF18) used as controls. This is the first report of the presence of recombined PVY in Argentina using a combination of biological, serological and molecular tools that shed light on the genetic diversity of PVY viruses in this country. |
| format | info:ar-repo/semantics/artículo |
| id | INTA3163 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Springer Netherlands |
| publisherStr | Springer Netherlands |
| record_format | dspace |
| spelling | INTA31632018-08-23T13:34:09Z Evidence of Recombinant Isolates of Potato Virus Y (PVY) in Argentina Colavita, Monica Lancia, Marcos Feingold, Sergio Enrique Massa, Gabriela Alejandra Papa Virus Diversidad Genética Recombinación Argentina Potatoes Viruses Genetic Diversity Recombination Potato Virus Y Virus Y Patata In order to make a first approach in the identification of the genetic diversity of Potato virus Y (PVY) in Argentina, 46 PVY isolates from different potato growing regions of Argentina were characterized both, biological and serologically. Five of them (ST11, RCA5a, RCA6, RCA14b and SSF6) were selected for further genomic analyses. Four genomic fragments containing hot spot regions of recombination (HSR) reported previously were sequenced in each isolate and compared to PVYN (CS434575.1) and PVYO (U09509.1) reference genomes looking for genomic recombinations. Isolates with one, two or three recombination points were identified among these, including the two strains considered typical PVYN (RCA5b) and PVYO (SSF18) used as controls. This is the first report of the presence of recombined PVY in Argentina using a combination of biological, serological and molecular tools that shed light on the genetic diversity of PVY viruses in this country. EEA Balcarce Fil: Colavita, Mónica L. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias, Balcarce; Argentina Fil: Lancia, Marcos. Universidad Nacional de Mar del Plata, Facultad de Ciencias Exactas y Naturales; Argentina. Universidad Nacional de La Plata. Instituto de Biotecnología y Biología Molecular, La Plata; Argentina Fil: Feingold, Sergio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Massa, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata, Facultad de Ciencias Agrarias, Balcarce; Argentina 2018-08-23T13:27:49Z 2018-08-23T13:27:49Z 2017-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007/s12230-016-9564-y http://hdl.handle.net/20.500.12123/3163 1099-209X 1874-9380 https://doi.org/10.1007/s12230-016-9564-y eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Netherlands American Journal of Potato Research 94 (3) : 275-282 (June 2017 ) |
| spellingShingle | Papa Virus Diversidad Genética Recombinación Argentina Potatoes Viruses Genetic Diversity Recombination Potato Virus Y Virus Y Patata Colavita, Monica Lancia, Marcos Feingold, Sergio Enrique Massa, Gabriela Alejandra Evidence of Recombinant Isolates of Potato Virus Y (PVY) in Argentina |
| title | Evidence of Recombinant Isolates of Potato Virus Y (PVY) in Argentina |
| title_full | Evidence of Recombinant Isolates of Potato Virus Y (PVY) in Argentina |
| title_fullStr | Evidence of Recombinant Isolates of Potato Virus Y (PVY) in Argentina |
| title_full_unstemmed | Evidence of Recombinant Isolates of Potato Virus Y (PVY) in Argentina |
| title_short | Evidence of Recombinant Isolates of Potato Virus Y (PVY) in Argentina |
| title_sort | evidence of recombinant isolates of potato virus y pvy in argentina |
| topic | Papa Virus Diversidad Genética Recombinación Argentina Potatoes Viruses Genetic Diversity Recombination Potato Virus Y Virus Y Patata |
| url | https://link.springer.com/article/10.1007/s12230-016-9564-y http://hdl.handle.net/20.500.12123/3163 https://doi.org/10.1007/s12230-016-9564-y |
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