Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system
OBJECTIVES: The use of induced pluripotent stem (iPS) cells as an alternative to embryonic stem cells to produce transgenic animals requires the development of a biotechnological platform for their generation. In this study, different strategies for the generation of bovine and porcine iPS cells wer...
| Autores principales: | , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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BioMedCentral
2018
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| Materias: | |
| Acceso en línea: | https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-018-3627-8 http://hdl.handle.net/20.500.12123/3086 https://doi.org/10.1186/s13104-018-3627-8 |
| _version_ | 1855483176289304576 |
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| author | Canizo, Jésica Romina Vazquez Echegaray, Camila Klisch, Doris Aller Atucha, Juan Florencio Paz, Dante Alberio, Ricardo Alberio, Ramiro Guberman, Alejandra |
| author_browse | Alberio, Ramiro Alberio, Ricardo Aller Atucha, Juan Florencio Canizo, Jésica Romina Guberman, Alejandra Klisch, Doris Paz, Dante Vazquez Echegaray, Camila |
| author_facet | Canizo, Jésica Romina Vazquez Echegaray, Camila Klisch, Doris Aller Atucha, Juan Florencio Paz, Dante Alberio, Ricardo Alberio, Ramiro Guberman, Alejandra |
| author_sort | Canizo, Jésica Romina |
| collection | INTA Digital |
| description | OBJECTIVES: The use of induced pluripotent stem (iPS) cells as an alternative to embryonic stem cells to produce transgenic animals requires the development of a biotechnological platform for their generation. In this study, different strategies for the generation of bovine and porcine iPS cells were evaluated. Lentiviral vectors were used to deliver human factors OCT4, SOX2, KLF4 and c-MYC (OKSM) into bovine and porcine embryonic fibroblasts and different culture conditions were evaluated.
RESULTS: Protocols based on the integrative lentiviral vector STEMCCA produced porcine iPS-like cells more efficiently than in bovine cells. The iPS-like cells generated displayed stem cell features; however, expression of exogenous factors was maintained along at least 12 passages. Since inactivation of the exogenous factors is still a major bottleneck for establishing fully reprogrammed iPS cells, defining culture conditions that support endogenous OKSM expression is critical for the efficient generation of farm animals' iPS cells. |
| format | Artículo |
| id | INTA3086 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | BioMedCentral |
| publisherStr | BioMedCentral |
| record_format | dspace |
| spelling | INTA30862018-08-15T15:49:48Z Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system Canizo, Jésica Romina Vazquez Echegaray, Camila Klisch, Doris Aller Atucha, Juan Florencio Paz, Dante Alberio, Ricardo Alberio, Ramiro Guberman, Alejandra Biotecnología Animales Transgénicos Células Fibroblastos Vectores Genéticos Biotechnology Transgenic Animals Cells Fibroblasts Genetic Vectors Bovino Porcino OBJECTIVES: The use of induced pluripotent stem (iPS) cells as an alternative to embryonic stem cells to produce transgenic animals requires the development of a biotechnological platform for their generation. In this study, different strategies for the generation of bovine and porcine iPS cells were evaluated. Lentiviral vectors were used to deliver human factors OCT4, SOX2, KLF4 and c-MYC (OKSM) into bovine and porcine embryonic fibroblasts and different culture conditions were evaluated. RESULTS: Protocols based on the integrative lentiviral vector STEMCCA produced porcine iPS-like cells more efficiently than in bovine cells. The iPS-like cells generated displayed stem cell features; however, expression of exogenous factors was maintained along at least 12 passages. Since inactivation of the exogenous factors is still a major bottleneck for establishing fully reprogrammed iPS cells, defining culture conditions that support endogenous OKSM expression is critical for the efficient generation of farm animals' iPS cells. EEA Balcarce Fil: Canizo, Jesica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Vazquez Echegaray, Camila. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Klisch, Doris. University of Nottingham. School of Biosciences; Gran Bretaña Fil: Aller Atucha, Juan. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Paz, Dante. Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales Departamento de Biodiversidad y Biología Experimental, Buenos Aires, Argentina. Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE), Universidad de Buenos Aires. Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires; Argentina Fil: Alberio, Ricardo. INTA Estación Experimental Agropecuaria Balcarce, Buenos Aires; Argentina Universidad Nacional de Mar del Plata, Facultad de Ciencias Agrarias, Balcarce; Argentina Fil: Alberio, Ramiro. University of Nottingham, School of Biosciences, Nottingham; Gran Bretaña Fil: Guberman, Alejandra. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Instituto de Química Biológica. Departamento de Fisiología y Biología Molecular y Celular; Argentina 2018-08-15T15:37:34Z 2018-08-15T15:37:34Z 2018-07-27 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-018-3627-8 http://hdl.handle.net/20.500.12123/3086 1756-0500 https://doi.org/10.1186/s13104-018-3627-8 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf BioMedCentral BMC Research Notes 11 : 509 (2018) |
| spellingShingle | Biotecnología Animales Transgénicos Células Fibroblastos Vectores Genéticos Biotechnology Transgenic Animals Cells Fibroblasts Genetic Vectors Bovino Porcino Canizo, Jésica Romina Vazquez Echegaray, Camila Klisch, Doris Aller Atucha, Juan Florencio Paz, Dante Alberio, Ricardo Alberio, Ramiro Guberman, Alejandra Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system |
| title | Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system |
| title_full | Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system |
| title_fullStr | Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system |
| title_full_unstemmed | Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system |
| title_short | Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system |
| title_sort | exogenous human oksm factors maintain pluripotency gene expression of bovine and porcine ips like cells obtained with stemcca delivery system |
| topic | Biotecnología Animales Transgénicos Células Fibroblastos Vectores Genéticos Biotechnology Transgenic Animals Cells Fibroblasts Genetic Vectors Bovino Porcino |
| url | https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-018-3627-8 http://hdl.handle.net/20.500.12123/3086 https://doi.org/10.1186/s13104-018-3627-8 |
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