Intramammary infusion of Panax ginseng extract in the bovine mammary gland at cessation of milking modifies components of the insulin-like growth factor system during involution
The objective of this study was to evaluate the effects of a single intramammary infusion of Panax ginseng extract (GS) on insulin-like growth factors (IGF) in bovine mammary gland during early involution. Eight mammary quarters from six nonpregnant cows in late lactation were infused with 10 mL of...
| Autores principales: | , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S0034528813000118 http://hdl.handle.net/20.500.12123/3074 https://doi.org/10.1016/j.rvsc.2013.01.009 |
| _version_ | 1855035078547079168 |
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| author | Dallard, Bibiana Elisabet Pujato, Silvina A. Baravalle, Celina Pereyra, Elizabet Amanda Lorena Rey, Florencia Renna, Maria Sol Calvinho, Luis Fernando |
| author_browse | Baravalle, Celina Calvinho, Luis Fernando Dallard, Bibiana Elisabet Pereyra, Elizabet Amanda Lorena Pujato, Silvina A. Renna, Maria Sol Rey, Florencia |
| author_facet | Dallard, Bibiana Elisabet Pujato, Silvina A. Baravalle, Celina Pereyra, Elizabet Amanda Lorena Rey, Florencia Renna, Maria Sol Calvinho, Luis Fernando |
| author_sort | Dallard, Bibiana Elisabet |
| collection | INTA Digital |
| description | The objective of this study was to evaluate the effects of a single intramammary infusion of Panax ginseng extract (GS) on insulin-like growth factors (IGF) in bovine mammary gland during early involution. Eight mammary quarters from six nonpregnant cows in late lactation were infused with 10 mL of ginseng extract solution (3 mg/mL), six quarters were treated with 10 mL of placebo (vehicle alone) and six quarters were maintained as uninoculated controls. Milking was interrupted after infusion. Concentrations of IGF1 in mammary secretions were higher in GS-treated quarters than in placebo and uninoculated control quarters at 24, 48 and 72 h post-treatment (p < 0.05). Treatment with GS did not affect mammary secretion of IGF2 (p = 0.942). At 7 d of post-lactational involution, a decrease of immunostained area and mRNA expression for IGF1 was observed in mammary tissue of GS-treated quarters compared with placebo-treated quarters and uninoculated controls (p < 0.05). The IGF2 immunostained area and mRNA expression for this growth factor were not affected by GS treatment (p = 0.216 and p = 0.785, respectively). An increase in protein levels and mRNA expression in mammary tissue of IGFBP3, IGFBP4 and IGFBP5 was observed in GS-treated quarters compared with placebo-treated quarters and uninoculated controls (p < 0.05). These results provide evidence that intramammary inoculation of GS extract at cessation of milking may promote early mammary involution through the inhibition of IGF1 local production and bioavailability. |
| format | info:ar-repo/semantics/artículo |
| id | INTA3074 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA30742018-08-15T12:12:28Z Intramammary infusion of Panax ginseng extract in the bovine mammary gland at cessation of milking modifies components of the insulin-like growth factor system during involution Dallard, Bibiana Elisabet Pujato, Silvina A. Baravalle, Celina Pereyra, Elizabet Amanda Lorena Rey, Florencia Renna, Maria Sol Calvinho, Luis Fernando Panax pseudoginseng Extractos Glándulas Mamarias Ganado Bovino Ordeño Factor de Crecimiento Igf Extracts Mammary Glands Cattle Milking Insulin-like Growth Factor Panax ginseng The objective of this study was to evaluate the effects of a single intramammary infusion of Panax ginseng extract (GS) on insulin-like growth factors (IGF) in bovine mammary gland during early involution. Eight mammary quarters from six nonpregnant cows in late lactation were infused with 10 mL of ginseng extract solution (3 mg/mL), six quarters were treated with 10 mL of placebo (vehicle alone) and six quarters were maintained as uninoculated controls. Milking was interrupted after infusion. Concentrations of IGF1 in mammary secretions were higher in GS-treated quarters than in placebo and uninoculated control quarters at 24, 48 and 72 h post-treatment (p < 0.05). Treatment with GS did not affect mammary secretion of IGF2 (p = 0.942). At 7 d of post-lactational involution, a decrease of immunostained area and mRNA expression for IGF1 was observed in mammary tissue of GS-treated quarters compared with placebo-treated quarters and uninoculated controls (p < 0.05). The IGF2 immunostained area and mRNA expression for this growth factor were not affected by GS treatment (p = 0.216 and p = 0.785, respectively). An increase in protein levels and mRNA expression in mammary tissue of IGFBP3, IGFBP4 and IGFBP5 was observed in GS-treated quarters compared with placebo-treated quarters and uninoculated controls (p < 0.05). These results provide evidence that intramammary inoculation of GS extract at cessation of milking may promote early mammary involution through the inhibition of IGF1 local production and bioavailability. EEA Rafaela Fil: Dallard, Bibiana Elisabet. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina. Concejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pujato, Silvina A.. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina Fil: Baravalle, Celina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina. Concejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pereyra, Elizabet Amanda Lorena. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina. Concejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rey, Florencia. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina. Concejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Renna, Maria Sol. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina. Concejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina 2018-08-15T12:10:58Z 2018-08-15T12:10:58Z 2013-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0034528813000118 http://hdl.handle.net/20.500.12123/3074 0034-5288 https://doi.org/10.1016/j.rvsc.2013.01.009 eng info:eu-repo/semantics/restrictedAccess application/pdf Research in Veterinary Science 94 (3) : 462-470 (June 2013) |
| spellingShingle | Panax pseudoginseng Extractos Glándulas Mamarias Ganado Bovino Ordeño Factor de Crecimiento Igf Extracts Mammary Glands Cattle Milking Insulin-like Growth Factor Panax ginseng Dallard, Bibiana Elisabet Pujato, Silvina A. Baravalle, Celina Pereyra, Elizabet Amanda Lorena Rey, Florencia Renna, Maria Sol Calvinho, Luis Fernando Intramammary infusion of Panax ginseng extract in the bovine mammary gland at cessation of milking modifies components of the insulin-like growth factor system during involution |
| title | Intramammary infusion of Panax ginseng extract in the bovine mammary gland at cessation of milking modifies components of the insulin-like growth factor system during involution |
| title_full | Intramammary infusion of Panax ginseng extract in the bovine mammary gland at cessation of milking modifies components of the insulin-like growth factor system during involution |
| title_fullStr | Intramammary infusion of Panax ginseng extract in the bovine mammary gland at cessation of milking modifies components of the insulin-like growth factor system during involution |
| title_full_unstemmed | Intramammary infusion of Panax ginseng extract in the bovine mammary gland at cessation of milking modifies components of the insulin-like growth factor system during involution |
| title_short | Intramammary infusion of Panax ginseng extract in the bovine mammary gland at cessation of milking modifies components of the insulin-like growth factor system during involution |
| title_sort | intramammary infusion of panax ginseng extract in the bovine mammary gland at cessation of milking modifies components of the insulin like growth factor system during involution |
| topic | Panax pseudoginseng Extractos Glándulas Mamarias Ganado Bovino Ordeño Factor de Crecimiento Igf Extracts Mammary Glands Cattle Milking Insulin-like Growth Factor Panax ginseng |
| url | https://www.sciencedirect.com/science/article/pii/S0034528813000118 http://hdl.handle.net/20.500.12123/3074 https://doi.org/10.1016/j.rvsc.2013.01.009 |
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