Short‐term hybridisation activates Tnt1 and Tto1 Copia retrotransposons in wild tuber‐bearing Solanum species
Interspecific hybridisation in tuber‐bearing species of Solanum is a common phenomenon and represents an important source of variability, crucial for adaptation and speciation of potato species. In this regard, the effects of interspecific hybridisation on retrotransposon families present in the gen...
| Autores principales: | , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
|
| Materias: | |
| Acceso en línea: | https://onlinelibrary.wiley.com/doi/abs/10.1111/plb.12301 http://hdl.handle.net/20.500.12123/3066 https://doi.org/10.1111/plb.12301 |
| _version_ | 1855483172864655360 |
|---|---|
| author | Paz, Rosalia Cristina Rendina González, Alejandra Pilar Ferrer, M.S. Masuelli, Ricardo Williams |
| author_browse | Ferrer, M.S. Masuelli, Ricardo Williams Paz, Rosalia Cristina Rendina González, Alejandra Pilar |
| author_facet | Paz, Rosalia Cristina Rendina González, Alejandra Pilar Ferrer, M.S. Masuelli, Ricardo Williams |
| author_sort | Paz, Rosalia Cristina |
| collection | INTA Digital |
| description | Interspecific hybridisation in tuber‐bearing species of Solanum is a common phenomenon and represents an important source of variability, crucial for adaptation and speciation of potato species. In this regard, the effects of interspecific hybridisation on retrotransposon families present in the genomes, and their consequent effects on generation of genetic variability in wild tuber‐bearing Solanum species, are poorly characterised. The aim of this study was to analyse the activity of retrotransposons in inter‐ and intraspecific hybrids between S. kurtzianum and S. microdontum, obtained by controlled crosses, and the effects on morphological, genetic and epigenetic variability. For genetic and epigenetic analysis, S‐SAP (sequence‐specific amplification polymorphism) and TMD (transposon methylation display) techniques were used, respectively, with specific primers for Tnt1 and Tto1 retrotransposon families (Order LTR, Superfamily Copia). The results indicate that at morphological level, interspecific hybrid genotypes differ from their parental species, whereas derived intraspecific hybrids do not. In both cases, we observed significant reductions in pollen grain viability, and a negative correlation with Tnt1 mobility. Both retrotransposons, Tto1 and Tnt1, were mobilised in the genotypes analysed, with mobility ranging from 0 to 7.8%. Furthermore, at the epigenetic level, demethylation was detected in the vicinity of Tnt1 and Tto1 in the hybrids compared with the parental genotypes. These patterns were positively correlated with the activity of the retrotransposons. The results suggest a possible mechanism through which hybridisation events generate genetic variability in tuber‐bearing species of Solanum through retrotranposon activation. |
| format | Artículo |
| id | INTA3066 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA30662018-08-14T14:44:34Z Short‐term hybridisation activates Tnt1 and Tto1 Copia retrotransposons in wild tuber‐bearing Solanum species Paz, Rosalia Cristina Rendina González, Alejandra Pilar Ferrer, M.S. Masuelli, Ricardo Williams Solanum Hibridación Interespecífica Variación Genética Transposones Genética Interspecific Hybridization Genetic Variation Transposons Genetics Elementos Genéticos Transponibles Interspecific hybridisation in tuber‐bearing species of Solanum is a common phenomenon and represents an important source of variability, crucial for adaptation and speciation of potato species. In this regard, the effects of interspecific hybridisation on retrotransposon families present in the genomes, and their consequent effects on generation of genetic variability in wild tuber‐bearing Solanum species, are poorly characterised. The aim of this study was to analyse the activity of retrotransposons in inter‐ and intraspecific hybrids between S. kurtzianum and S. microdontum, obtained by controlled crosses, and the effects on morphological, genetic and epigenetic variability. For genetic and epigenetic analysis, S‐SAP (sequence‐specific amplification polymorphism) and TMD (transposon methylation display) techniques were used, respectively, with specific primers for Tnt1 and Tto1 retrotransposon families (Order LTR, Superfamily Copia). The results indicate that at morphological level, interspecific hybrid genotypes differ from their parental species, whereas derived intraspecific hybrids do not. In both cases, we observed significant reductions in pollen grain viability, and a negative correlation with Tnt1 mobility. Both retrotransposons, Tto1 and Tnt1, were mobilised in the genotypes analysed, with mobility ranging from 0 to 7.8%. Furthermore, at the epigenetic level, demethylation was detected in the vicinity of Tnt1 and Tto1 in the hybrids compared with the parental genotypes. These patterns were positively correlated with the activity of the retrotransposons. The results suggest a possible mechanism through which hybridisation events generate genetic variability in tuber‐bearing species of Solanum through retrotranposon activation. EEA La Consulta Fil: Paz, Rosalia Cristina. Universidad Nacional de San Juan. Departamento de Biología. Grupo INTERBIODES; Argentina Fil: Rendina González, Alejandra Pilar. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina Fil: Ferrer, M.S. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina Fil: Masuelli, Ricardo Williams. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina 2018-08-14T14:42:31Z 2018-08-14T14:42:31Z 2015-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://onlinelibrary.wiley.com/doi/abs/10.1111/plb.12301 http://hdl.handle.net/20.500.12123/3066 1435-8603 1438-8677 https://doi.org/10.1111/plb.12301 eng info:eu-repo/semantics/restrictedAccess application/pdf Plant Biology 17 (4) : 860-869 (July 2015) |
| spellingShingle | Solanum Hibridación Interespecífica Variación Genética Transposones Genética Interspecific Hybridization Genetic Variation Transposons Genetics Elementos Genéticos Transponibles Paz, Rosalia Cristina Rendina González, Alejandra Pilar Ferrer, M.S. Masuelli, Ricardo Williams Short‐term hybridisation activates Tnt1 and Tto1 Copia retrotransposons in wild tuber‐bearing Solanum species |
| title | Short‐term hybridisation activates Tnt1 and Tto1 Copia retrotransposons in wild tuber‐bearing Solanum species |
| title_full | Short‐term hybridisation activates Tnt1 and Tto1 Copia retrotransposons in wild tuber‐bearing Solanum species |
| title_fullStr | Short‐term hybridisation activates Tnt1 and Tto1 Copia retrotransposons in wild tuber‐bearing Solanum species |
| title_full_unstemmed | Short‐term hybridisation activates Tnt1 and Tto1 Copia retrotransposons in wild tuber‐bearing Solanum species |
| title_short | Short‐term hybridisation activates Tnt1 and Tto1 Copia retrotransposons in wild tuber‐bearing Solanum species |
| title_sort | short term hybridisation activates tnt1 and tto1 copia retrotransposons in wild tuber bearing solanum species |
| topic | Solanum Hibridación Interespecífica Variación Genética Transposones Genética Interspecific Hybridization Genetic Variation Transposons Genetics Elementos Genéticos Transponibles |
| url | https://onlinelibrary.wiley.com/doi/abs/10.1111/plb.12301 http://hdl.handle.net/20.500.12123/3066 https://doi.org/10.1111/plb.12301 |
| work_keys_str_mv | AT pazrosaliacristina shorttermhybridisationactivatestnt1andtto1copiaretrotransposonsinwildtuberbearingsolanumspecies AT rendinagonzalezalejandrapilar shorttermhybridisationactivatestnt1andtto1copiaretrotransposonsinwildtuberbearingsolanumspecies AT ferrerms shorttermhybridisationactivatestnt1andtto1copiaretrotransposonsinwildtuberbearingsolanumspecies AT masuelliricardowilliams shorttermhybridisationactivatestnt1andtto1copiaretrotransposonsinwildtuberbearingsolanumspecies |