Comparative proteomics for the characterization of the most relevant Amblyomma tick species as vectors of zoonotic pathogens worldwide
Ticks transmit zoonotic pathogens worldwide. Nevertheless, very little information is available on their genome, transcriptome and proteome. Herein, we characterized the proteome of Amblyomma americanum adults and nymphs because of their role in pathogen transmission and compared the proteome of A....
| Main Authors: | , , , |
|---|---|
| Format: | Artículo |
| Language: | Inglés |
| Published: |
2018
|
| Subjects: | |
| Online Access: | https://www.sciencedirect.com/science/article/pii/S1874391913006519 http://hdl.handle.net/20.500.12123/3039 https://doi.org/10.1016/j.jprot.2013.12.016 |
| _version_ | 1855483167713001472 |
|---|---|
| author | Villar, Margarita Popara, Marina Mangold, Atilio Jose Fuente, José de la |
| author_browse | Fuente, José de la Mangold, Atilio Jose Popara, Marina Villar, Margarita |
| author_facet | Villar, Margarita Popara, Marina Mangold, Atilio Jose Fuente, José de la |
| author_sort | Villar, Margarita |
| collection | INTA Digital |
| description | Ticks transmit zoonotic pathogens worldwide. Nevertheless, very little information is available on their genome, transcriptome and proteome. Herein, we characterized the proteome of Amblyomma americanum adults and nymphs because of their role in pathogen transmission and compared the proteome of A. americanum, A. cajennense and A. variegatum adult ticks. We also used de novo sequencing proteomics data for the analysis of the phylogenetic relationships between the three Amblyomma spp. in a proof of concept for phyloproteomics. The results showed that host and tick proteins involved in blood digestion, heme detoxification, development and innate immunity were differentially represented between adults and nymphs. Although these ticks were unfed, over-represented host proteins may supply nutrients during off-host periods. Tick proteins involved in tick attachment, feeding, heat shock response, protease inhibition and heme detoxification were differentially represented between Amblyomma spp., suggesting adaptation processes to biotic and abiotic factors. These results suggested that phyloproteomics might be a useful tool for the phylogenetic analysis of tick species in which sequence data is a limiting factor and demonstrate the possibilities of proteomics studies for the characterization of relevant tick vector species and provide new relevant information to understand the physiology, development and evolution of these tick species. |
| format | Artículo |
| id | INTA3039 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA30392018-08-10T11:50:00Z Comparative proteomics for the characterization of the most relevant Amblyomma tick species as vectors of zoonotic pathogens worldwide Villar, Margarita Popara, Marina Mangold, Atilio Jose Fuente, José de la Amblyomma Vectores Zoonosis Huéspedes Vectors Zoonoses Hosts Garrapatas Proteómica Ticks transmit zoonotic pathogens worldwide. Nevertheless, very little information is available on their genome, transcriptome and proteome. Herein, we characterized the proteome of Amblyomma americanum adults and nymphs because of their role in pathogen transmission and compared the proteome of A. americanum, A. cajennense and A. variegatum adult ticks. We also used de novo sequencing proteomics data for the analysis of the phylogenetic relationships between the three Amblyomma spp. in a proof of concept for phyloproteomics. The results showed that host and tick proteins involved in blood digestion, heme detoxification, development and innate immunity were differentially represented between adults and nymphs. Although these ticks were unfed, over-represented host proteins may supply nutrients during off-host periods. Tick proteins involved in tick attachment, feeding, heat shock response, protease inhibition and heme detoxification were differentially represented between Amblyomma spp., suggesting adaptation processes to biotic and abiotic factors. These results suggested that phyloproteomics might be a useful tool for the phylogenetic analysis of tick species in which sequence data is a limiting factor and demonstrate the possibilities of proteomics studies for the characterization of relevant tick vector species and provide new relevant information to understand the physiology, development and evolution of these tick species. EEA Rafaela Fil: Villar, Margarita. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC). SaBio; España Fil: Popara, Marina. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC). SaBio; España Fil: Mangold, Atilio Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Fuente, José de la. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC). SaBio; España. Oklahoma State University. Center for Veterinary Health Sciences. Department of Veterinary Pathobiology; Estados Unidos 2018-08-10T11:48:28Z 2018-08-10T11:48:28Z 2014-06-13 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S1874391913006519 http://hdl.handle.net/20.500.12123/3039 1874-3919 1876-7737 https://doi.org/10.1016/j.jprot.2013.12.016 eng info:eu-repo/semantics/restrictedAccess application/pdf Journal of Proteomics 105 : 204-216 (June 2014) |
| spellingShingle | Amblyomma Vectores Zoonosis Huéspedes Vectors Zoonoses Hosts Garrapatas Proteómica Villar, Margarita Popara, Marina Mangold, Atilio Jose Fuente, José de la Comparative proteomics for the characterization of the most relevant Amblyomma tick species as vectors of zoonotic pathogens worldwide |
| title | Comparative proteomics for the characterization of the most relevant Amblyomma tick species as vectors of zoonotic pathogens worldwide |
| title_full | Comparative proteomics for the characterization of the most relevant Amblyomma tick species as vectors of zoonotic pathogens worldwide |
| title_fullStr | Comparative proteomics for the characterization of the most relevant Amblyomma tick species as vectors of zoonotic pathogens worldwide |
| title_full_unstemmed | Comparative proteomics for the characterization of the most relevant Amblyomma tick species as vectors of zoonotic pathogens worldwide |
| title_short | Comparative proteomics for the characterization of the most relevant Amblyomma tick species as vectors of zoonotic pathogens worldwide |
| title_sort | comparative proteomics for the characterization of the most relevant amblyomma tick species as vectors of zoonotic pathogens worldwide |
| topic | Amblyomma Vectores Zoonosis Huéspedes Vectors Zoonoses Hosts Garrapatas Proteómica |
| url | https://www.sciencedirect.com/science/article/pii/S1874391913006519 http://hdl.handle.net/20.500.12123/3039 https://doi.org/10.1016/j.jprot.2013.12.016 |
| work_keys_str_mv | AT villarmargarita comparativeproteomicsforthecharacterizationofthemostrelevantamblyommatickspeciesasvectorsofzoonoticpathogensworldwide AT poparamarina comparativeproteomicsforthecharacterizationofthemostrelevantamblyommatickspeciesasvectorsofzoonoticpathogensworldwide AT mangoldatiliojose comparativeproteomicsforthecharacterizationofthemostrelevantamblyommatickspeciesasvectorsofzoonoticpathogensworldwide AT fuentejosedela comparativeproteomicsforthecharacterizationofthemostrelevantamblyommatickspeciesasvectorsofzoonoticpathogensworldwide |