Biochemical characterization of thioredoxin reductase from Babesia bovis
This paper addresses the identification, cloning, expression, purification and functional characterization of thioredoxin reductase from Babesia bovis, the etiological agent of babesiosis. The work deals with in vitro steady state kinetic studies and other complementary analyses of the thioredoxin r...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S0300908413003982 http://hdl.handle.net/20.500.12123/3029 https://doi.org/10.1016/j.biochi.2013.11.002 |
| _version_ | 1855483166177886208 |
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| author | Regner, Erika Lucía Thompson, Carolina Soledad Iglesias, Alberto Alvaro Guerrero, Sergio Adrian Arias, Diego Gustavo |
| author_browse | Arias, Diego Gustavo Guerrero, Sergio Adrian Iglesias, Alberto Alvaro Regner, Erika Lucía Thompson, Carolina Soledad |
| author_facet | Regner, Erika Lucía Thompson, Carolina Soledad Iglesias, Alberto Alvaro Guerrero, Sergio Adrian Arias, Diego Gustavo |
| author_sort | Regner, Erika Lucía |
| collection | INTA Digital |
| description | This paper addresses the identification, cloning, expression, purification and functional characterization of thioredoxin reductase from Babesia bovis, the etiological agent of babesiosis. The work deals with in vitro steady state kinetic studies and other complementary analyses of the thioredoxin reductase found in the pathogenic protist. Thioredoxin reductase from B. bovis was characterized as a homodimeric flavoprotein that catalyzes the NADPH-dependent reduction of Trx with a high catalytic efficiency. Moreover, the enzyme exhibited a disulfide reductase activity using DTNB as substrate, being this activity highly sensitive to inhibition by Eosin B. The thioredoxin reductase/thioredoxin system can reduce oxidized glutathione and S-nitrosoglutathione. Our in vitro data suggest that antioxidant defense in B. bovis could be supported by this enzyme. We have performed an enzymatic characterization, searching for targets for rational design of inhibitors. This work contributes to the better understanding of the redox biochemistry occurring in the parasite. |
| format | Artículo |
| id | INTA3029 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA30292018-08-09T13:20:46Z Biochemical characterization of thioredoxin reductase from Babesia bovis Regner, Erika Lucía Thompson, Carolina Soledad Iglesias, Alberto Alvaro Guerrero, Sergio Adrian Arias, Diego Gustavo Babesia bovis Babesiosis Enfermedades de los Animales Etiología Glutation Animal Diseases Aetiology Glutathione Thioredoxin This paper addresses the identification, cloning, expression, purification and functional characterization of thioredoxin reductase from Babesia bovis, the etiological agent of babesiosis. The work deals with in vitro steady state kinetic studies and other complementary analyses of the thioredoxin reductase found in the pathogenic protist. Thioredoxin reductase from B. bovis was characterized as a homodimeric flavoprotein that catalyzes the NADPH-dependent reduction of Trx with a high catalytic efficiency. Moreover, the enzyme exhibited a disulfide reductase activity using DTNB as substrate, being this activity highly sensitive to inhibition by Eosin B. The thioredoxin reductase/thioredoxin system can reduce oxidized glutathione and S-nitrosoglutathione. Our in vitro data suggest that antioxidant defense in B. bovis could be supported by this enzyme. We have performed an enzymatic characterization, searching for targets for rational design of inhibitors. This work contributes to the better understanding of the redox biochemistry occurring in the parasite. EEA Rafaela Fil: Regner, Erika Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Agrobiotecnologia del Litoral. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; Argentina Fil: Thompson, Carolina Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Iglesias, Alberto Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Agrobiotecnologia del Litoral. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; Argentina Fil: Guerrero, Sergio Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Agrobiotecnologia del Litoral. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; Argentina Fil: Arias, Diego Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Agrobiotecnologia del Litoral. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; Argentina 2018-08-09T13:19:28Z 2018-08-09T13:19:28Z 2014-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0300908413003982 http://hdl.handle.net/20.500.12123/3029 0300-9084 https://doi.org/10.1016/j.biochi.2013.11.002 eng info:eu-repo/semantics/restrictedAccess application/pdf Biochimie 99 : 44-53 (April 2014) |
| spellingShingle | Babesia bovis Babesiosis Enfermedades de los Animales Etiología Glutation Animal Diseases Aetiology Glutathione Thioredoxin Regner, Erika Lucía Thompson, Carolina Soledad Iglesias, Alberto Alvaro Guerrero, Sergio Adrian Arias, Diego Gustavo Biochemical characterization of thioredoxin reductase from Babesia bovis |
| title | Biochemical characterization of thioredoxin reductase from Babesia bovis |
| title_full | Biochemical characterization of thioredoxin reductase from Babesia bovis |
| title_fullStr | Biochemical characterization of thioredoxin reductase from Babesia bovis |
| title_full_unstemmed | Biochemical characterization of thioredoxin reductase from Babesia bovis |
| title_short | Biochemical characterization of thioredoxin reductase from Babesia bovis |
| title_sort | biochemical characterization of thioredoxin reductase from babesia bovis |
| topic | Babesia bovis Babesiosis Enfermedades de los Animales Etiología Glutation Animal Diseases Aetiology Glutathione Thioredoxin |
| url | https://www.sciencedirect.com/science/article/pii/S0300908413003982 http://hdl.handle.net/20.500.12123/3029 https://doi.org/10.1016/j.biochi.2013.11.002 |
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