Evaluation of the immune response to Anaplasma marginale MSP5 protein using a HSV-1 amplicon vector system or recombinant protein
Anaplasma marginale is an intraerythrocytic vector-borne infectious agent of cattle. Immunization with the current vaccine, based on parasitized erythrocytes with live Anaplasma centrale, shows some constraints and confers partial protection, suggesting the feasibility for the development of new gen...
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| Format: | Artículo |
| Language: | Inglés |
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2018
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| Online Access: | https://www.sciencedirect.com/science/article/pii/S003452881400294X http://hdl.handle.net/20.500.12123/3028 https://doi.org/10.1016/j.rvsc.2014.10.005 |
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| author | Palacios, Carlos Adolfo Torioni, Susana Marta Mattion, Nora Marta |
| author_browse | Mattion, Nora Marta Palacios, Carlos Adolfo Torioni, Susana Marta |
| author_facet | Palacios, Carlos Adolfo Torioni, Susana Marta Mattion, Nora Marta |
| author_sort | Palacios, Carlos Adolfo |
| collection | INTA Digital |
| description | Anaplasma marginale is an intraerythrocytic vector-borne infectious agent of cattle. Immunization with the current vaccine, based on parasitized erythrocytes with live Anaplasma centrale, shows some constraints and confers partial protection, suggesting the feasibility for the development of new generation of vaccines. The aim of the present study was to assess the effect of sequential immunization of BALB/c mice, with herpesvirus amplicon vector-based vaccines combined with protein-based vaccines, on the quality of the immune response against the major surface protein 5 of A. marginale. The highest antibody titers against MSP5 were elicited in mice that received two doses of adjuvanted recombinant protein (p < 0.0001). Mice treated with a heterologous prime-boost strategy generated sustained antibody titers at least up to 200 days, and a higher specific cellular response. The results presented here showed that sequential immunization with HSV-based vectors and purified antigen enhances the quality of the immune response against A. marginale. |
| format | Artículo |
| id | INTA3028 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA30282018-08-09T12:58:19Z Evaluation of the immune response to Anaplasma marginale MSP5 protein using a HSV-1 amplicon vector system or recombinant protein Palacios, Carlos Adolfo Torioni, Susana Marta Mattion, Nora Marta Anaplasma Marginale Respuesta Inmunológica Proteínas Recombinantes Vectores Ganado Bovino Immune Response Recombinant Proteins Vectors Cattle Anaplasma marginale is an intraerythrocytic vector-borne infectious agent of cattle. Immunization with the current vaccine, based on parasitized erythrocytes with live Anaplasma centrale, shows some constraints and confers partial protection, suggesting the feasibility for the development of new generation of vaccines. The aim of the present study was to assess the effect of sequential immunization of BALB/c mice, with herpesvirus amplicon vector-based vaccines combined with protein-based vaccines, on the quality of the immune response against the major surface protein 5 of A. marginale. The highest antibody titers against MSP5 were elicited in mice that received two doses of adjuvanted recombinant protein (p < 0.0001). Mice treated with a heterologous prime-boost strategy generated sustained antibody titers at least up to 200 days, and a higher specific cellular response. The results presented here showed that sequential immunization with HSV-based vectors and purified antigen enhances the quality of the immune response against A. marginale. EEA Rafaela Fil: Palacios, Carlos Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein". Centro de Virología Animal; Argentina Fil: Torioni, Susana Marta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Mattion, Nora Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein". Centro de Virología Animal; Argentina 2018-08-09T12:53:44Z 2018-08-09T12:53:44Z 2014-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S003452881400294X http://hdl.handle.net/20.500.12123/3028 0034-5288 https://doi.org/10.1016/j.rvsc.2014.10.005 eng info:eu-repo/semantics/restrictedAccess application/pdf Research in Veterinary Science 97 (3) : 514-520 (December 2014) |
| spellingShingle | Anaplasma Marginale Respuesta Inmunológica Proteínas Recombinantes Vectores Ganado Bovino Immune Response Recombinant Proteins Vectors Cattle Palacios, Carlos Adolfo Torioni, Susana Marta Mattion, Nora Marta Evaluation of the immune response to Anaplasma marginale MSP5 protein using a HSV-1 amplicon vector system or recombinant protein |
| title | Evaluation of the immune response to Anaplasma marginale MSP5 protein using a HSV-1 amplicon vector system or recombinant protein |
| title_full | Evaluation of the immune response to Anaplasma marginale MSP5 protein using a HSV-1 amplicon vector system or recombinant protein |
| title_fullStr | Evaluation of the immune response to Anaplasma marginale MSP5 protein using a HSV-1 amplicon vector system or recombinant protein |
| title_full_unstemmed | Evaluation of the immune response to Anaplasma marginale MSP5 protein using a HSV-1 amplicon vector system or recombinant protein |
| title_short | Evaluation of the immune response to Anaplasma marginale MSP5 protein using a HSV-1 amplicon vector system or recombinant protein |
| title_sort | evaluation of the immune response to anaplasma marginale msp5 protein using a hsv 1 amplicon vector system or recombinant protein |
| topic | Anaplasma Marginale Respuesta Inmunológica Proteínas Recombinantes Vectores Ganado Bovino Immune Response Recombinant Proteins Vectors Cattle |
| url | https://www.sciencedirect.com/science/article/pii/S003452881400294X http://hdl.handle.net/20.500.12123/3028 https://doi.org/10.1016/j.rvsc.2014.10.005 |
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