Molecular evidence of Babesia species in Procyon cancrivorus (Carnivora, Procyonidae) in Uruguay
The crab-eating raccoon (Procyon cancrivorus) is a carnivore widely distributed from southern Central America to all South American countries except Chile. In the Southern cone of America, P. cancrivorus has been found parasitized by several Amblyomma spp. Particularly, in Uruguay, A. aureolatum is...
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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2018
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| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S2405939017302770 http://hdl.handle.net/20.500.12123/3014 https://doi.org/10.1016/j.vprsr.2018.07.008 |
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| author | Thompson, Carolina Soledad Mangold, Atilio Jose Félix, María L. Carvalho, Luis A. Armúa-Fernández, María Teresita Venzal, José Manuel |
| author_browse | Armúa-Fernández, María Teresita Carvalho, Luis A. Félix, María L. Mangold, Atilio Jose Thompson, Carolina Soledad Venzal, José Manuel |
| author_facet | Thompson, Carolina Soledad Mangold, Atilio Jose Félix, María L. Carvalho, Luis A. Armúa-Fernández, María Teresita Venzal, José Manuel |
| author_sort | Thompson, Carolina Soledad |
| collection | INTA Digital |
| description | The crab-eating raccoon (Procyon cancrivorus) is a carnivore widely distributed from southern Central America to all South American countries except Chile. In the Southern cone of America, P. cancrivorus has been found parasitized by several Amblyomma spp. Particularly, in Uruguay, A. aureolatum is the only tick found in this wild carnivore. Piroplasmid hemoparasites were found in Procyon lotor from North America and Japan. In this work, molecular evidence Babesia sp. DNA was found in blood and tissues from road-killed P. cancrivorus from different locations in Uruguay. PCRs targeting 18S rRNA gene were carried out. Subsequently, the obtained amplicons were sequenced and full-length sequences was assembled. A phylogenetic tree was constructed and revealed that the Babesia sp. found in this work clustered with other 18sRNA sequences of Babesia spp. obtained from P. lotor from Japan and USA, along with Babesia spp. of maned wolf and I. ovatus. This is the first report of molecular evidence of Babesia sp. parasitizing P. cancrivorus. |
| format | info:ar-repo/semantics/artículo |
| id | INTA3014 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA30142019-01-22T17:25:06Z Molecular evidence of Babesia species in Procyon cancrivorus (Carnivora, Procyonidae) in Uruguay Thompson, Carolina Soledad Mangold, Atilio Jose Félix, María L. Carvalho, Luis A. Armúa-Fernández, María Teresita Venzal, José Manuel Procyon Babesia Amblyomma Relaciones Huésped Parásito Genética Host Parasite Relations Genetics Procyon cancrivorus Mapache Amblyomma aureolatum Uruguay The crab-eating raccoon (Procyon cancrivorus) is a carnivore widely distributed from southern Central America to all South American countries except Chile. In the Southern cone of America, P. cancrivorus has been found parasitized by several Amblyomma spp. Particularly, in Uruguay, A. aureolatum is the only tick found in this wild carnivore. Piroplasmid hemoparasites were found in Procyon lotor from North America and Japan. In this work, molecular evidence Babesia sp. DNA was found in blood and tissues from road-killed P. cancrivorus from different locations in Uruguay. PCRs targeting 18S rRNA gene were carried out. Subsequently, the obtained amplicons were sequenced and full-length sequences was assembled. A phylogenetic tree was constructed and revealed that the Babesia sp. found in this work clustered with other 18sRNA sequences of Babesia spp. obtained from P. lotor from Japan and USA, along with Babesia spp. of maned wolf and I. ovatus. This is the first report of molecular evidence of Babesia sp. parasitizing P. cancrivorus. EEA Rafaela Fil: Thompson, Carolina Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Mangold, Atilio Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Félix, María L. Universidad de la República. CENUR Litoral Norte-Salto. Facultad de Veterinaria. Laboratorio de Vectores y Enfermedades Transmitidas; Uruguay Fil: Carvalho, Luis A. Universidad de la República. CENUR Litoral Norte-Salto. Facultad de Veterinaria. Laboratorio de Vectores y Enfermedades Transmitidas; Uruguay Fil: Armúa-Fernández, María Teresita. Universidad de la República. CENUR Litoral Norte-Salto. Facultad de Veterinaria. Laboratorio de Vectores y Enfermedades Transmitidas; Uruguay Fil: Venzal, José Manuel. Universidad de la República. CENUR Litoral Norte-Salto. Facultad de Veterinaria. Laboratorio de Vectores y Enfermedades Transmitidas; Uruguay 2018-08-08T13:34:28Z 2018-08-08T13:34:28Z 2018-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S2405939017302770 http://hdl.handle.net/20.500.12123/3014 2405-9390 https://doi.org/10.1016/j.vprsr.2018.07.008 eng info:eu-repo/semantics/restrictedAccess application/pdf Uruguay (nation) Veterinary Parasitology: Regional Studies and Reports 13 : 230-233 (August 2018) |
| spellingShingle | Procyon Babesia Amblyomma Relaciones Huésped Parásito Genética Host Parasite Relations Genetics Procyon cancrivorus Mapache Amblyomma aureolatum Uruguay Thompson, Carolina Soledad Mangold, Atilio Jose Félix, María L. Carvalho, Luis A. Armúa-Fernández, María Teresita Venzal, José Manuel Molecular evidence of Babesia species in Procyon cancrivorus (Carnivora, Procyonidae) in Uruguay |
| title | Molecular evidence of Babesia species in Procyon cancrivorus (Carnivora, Procyonidae) in Uruguay |
| title_full | Molecular evidence of Babesia species in Procyon cancrivorus (Carnivora, Procyonidae) in Uruguay |
| title_fullStr | Molecular evidence of Babesia species in Procyon cancrivorus (Carnivora, Procyonidae) in Uruguay |
| title_full_unstemmed | Molecular evidence of Babesia species in Procyon cancrivorus (Carnivora, Procyonidae) in Uruguay |
| title_short | Molecular evidence of Babesia species in Procyon cancrivorus (Carnivora, Procyonidae) in Uruguay |
| title_sort | molecular evidence of babesia species in procyon cancrivorus carnivora procyonidae in uruguay |
| topic | Procyon Babesia Amblyomma Relaciones Huésped Parásito Genética Host Parasite Relations Genetics Procyon cancrivorus Mapache Amblyomma aureolatum Uruguay |
| url | https://www.sciencedirect.com/science/article/pii/S2405939017302770 http://hdl.handle.net/20.500.12123/3014 https://doi.org/10.1016/j.vprsr.2018.07.008 |
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