Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage
In Argentina, classical vaccines are used to control infectious bursal disease virus (IBDV); however, outbreaks of IBDV are frequently observed. This could be due to failures in the vaccination programs or to the emergence of new strains, which would be able to break through the protection given by...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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2018
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| Online Access: | https://link.springer.com/article/10.1007/s00705-015-2449-4 http://hdl.handle.net/20.500.12123/2968 https://doi.org/10.1007/s00705-015-2449-4 |
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| author | Vera, Federico Sebastian Craig, María Isabel Olivera, Valeria Soledad Rojas, María Florencia Konig, Guido Alberto Pereda, Ariel Julian Vagnozzi, Ariel Eduardo |
| author_browse | Craig, María Isabel Konig, Guido Alberto Olivera, Valeria Soledad Pereda, Ariel Julian Rojas, María Florencia Vagnozzi, Ariel Eduardo Vera, Federico Sebastian |
| author_facet | Vera, Federico Sebastian Craig, María Isabel Olivera, Valeria Soledad Rojas, María Florencia Konig, Guido Alberto Pereda, Ariel Julian Vagnozzi, Ariel Eduardo |
| author_sort | Vera, Federico Sebastian |
| collection | INTA Digital |
| description | In Argentina, classical vaccines are used to control infectious bursal disease virus (IBDV); however, outbreaks of IBDV are frequently observed. This could be due to failures in the vaccination programs or to the emergence of new strains, which would be able to break through the protection given by vaccines. Hence, genetic characterization of the viruses responsible for the outbreaks that occurred in recent years is crucial for the evaluation of the control programs and the understanding of the epidemiology and evolution of IBDV. In this study, we characterized 51 field samples collected in Argentina (previously identified as IBDV positive) through the analysis of previously identified apomorphic sequences. Phylogenetic analysis of regVP2 showed that 42 samples formed a unique cluster (Argentinean lineage), seven samples were typical classical strains (one of them was a vaccine strain), and two belonged to the very virulent lineage (vvIBDV). Interestingly, when the analysis was performed on the regVP1 sequences, the field samples segregated similarly to regVP2; thus, we observed no evidence of a reassortment event in the Argentinean samples. Amino acid sequence analysis of regVP2 showed a particular pattern of residues in the Argentinean lineage, particularly the presence of T272, P289 and F296, which had not been reported before as signature sequences for any IBDV phenotype. Notably, the residue S254, characteristic of the antigenic variant, was not present in any of the Argentinean samples. |
| format | info:ar-repo/semantics/artículo |
| id | INTA2968 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA29682018-08-03T13:14:34Z Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage Vera, Federico Sebastian Craig, María Isabel Olivera, Valeria Soledad Rojas, María Florencia Konig, Guido Alberto Pereda, Ariel Julian Vagnozzi, Ariel Eduardo Virus Bursitis Infecciosa Genética Vacuna Antígenos Epidemiología Infectious Bursal Disease Virus Genetics Vaccines Antigens Epidemiology Argentina In Argentina, classical vaccines are used to control infectious bursal disease virus (IBDV); however, outbreaks of IBDV are frequently observed. This could be due to failures in the vaccination programs or to the emergence of new strains, which would be able to break through the protection given by vaccines. Hence, genetic characterization of the viruses responsible for the outbreaks that occurred in recent years is crucial for the evaluation of the control programs and the understanding of the epidemiology and evolution of IBDV. In this study, we characterized 51 field samples collected in Argentina (previously identified as IBDV positive) through the analysis of previously identified apomorphic sequences. Phylogenetic analysis of regVP2 showed that 42 samples formed a unique cluster (Argentinean lineage), seven samples were typical classical strains (one of them was a vaccine strain), and two belonged to the very virulent lineage (vvIBDV). Interestingly, when the analysis was performed on the regVP1 sequences, the field samples segregated similarly to regVP2; thus, we observed no evidence of a reassortment event in the Argentinean samples. Amino acid sequence analysis of regVP2 showed a particular pattern of residues in the Argentinean lineage, particularly the presence of T272, P289 and F296, which had not been reported before as signature sequences for any IBDV phenotype. Notably, the residue S254, characteristic of the antigenic variant, was not present in any of the Argentinean samples. EEA Concepción del Uruguay Fil: Vera, Federico Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio Sanidad Aviar; Argentina Fil: Craig, María Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Olivera, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Rojas, María Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio Sanidad Aviar; Argentina Fil: Konig, Guido Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Pereda, Ariel Julian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Vagnozzi, Ariel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio Sanidad Aviar; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina 2018-08-03T13:13:11Z 2018-08-03T13:13:11Z 2015-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007/s00705-015-2449-4 http://hdl.handle.net/20.500.12123/2968 0304-8608 1432-8798 https://doi.org/10.1007/s00705-015-2449-4 eng info:eu-repo/semantics/restrictedAccess application/pdf Archives of Virology 160 (8) : 1909–1921 (August 2015) |
| spellingShingle | Virus Bursitis Infecciosa Genética Vacuna Antígenos Epidemiología Infectious Bursal Disease Virus Genetics Vaccines Antigens Epidemiology Argentina Vera, Federico Sebastian Craig, María Isabel Olivera, Valeria Soledad Rojas, María Florencia Konig, Guido Alberto Pereda, Ariel Julian Vagnozzi, Ariel Eduardo Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage |
| title | Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage |
| title_full | Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage |
| title_fullStr | Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage |
| title_full_unstemmed | Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage |
| title_short | Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage |
| title_sort | molecular characterization of infectious bursal disease virus ibdv isolated in argentina indicates a regional lineage |
| topic | Virus Bursitis Infecciosa Genética Vacuna Antígenos Epidemiología Infectious Bursal Disease Virus Genetics Vaccines Antigens Epidemiology Argentina |
| url | https://link.springer.com/article/10.1007/s00705-015-2449-4 http://hdl.handle.net/20.500.12123/2968 https://doi.org/10.1007/s00705-015-2449-4 |
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