Assessment of bacterial inoculant formulated with Paraburkholderia tropica to enhance wheat productivity
Paraburkholderia tropica is an endophytic nitrogen-fixing bacterium isolated from the rhizosphere, rhizoplane, and internal tissues of sugarcane and corn plants in different geographical regions. Other plant-growth-promoting abilities, such as phosphate solubilization and antifungal activity, have a...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Acceso en línea: | https://link.springer.com/article/10.1007/s11274-018-2461-4 http://hdl.handle.net/20.500.12123/2932 https://doi.org/10.1007/s11274-018-2461-4 |
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| author | Bernabeu, Pamela Romina Garcia, Sabrina Soledad López, Ana Clara Vio, Santiago Adolfo Carrasco, Natalia Boiardi, Jose Luis Luna, Maria Flavia |
| author_browse | Bernabeu, Pamela Romina Boiardi, Jose Luis Carrasco, Natalia Garcia, Sabrina Soledad Luna, Maria Flavia López, Ana Clara Vio, Santiago Adolfo |
| author_facet | Bernabeu, Pamela Romina Garcia, Sabrina Soledad López, Ana Clara Vio, Santiago Adolfo Carrasco, Natalia Boiardi, Jose Luis Luna, Maria Flavia |
| author_sort | Bernabeu, Pamela Romina |
| collection | INTA Digital |
| description | Paraburkholderia tropica is an endophytic nitrogen-fixing bacterium isolated from the rhizosphere, rhizoplane, and internal tissues of sugarcane and corn plants in different geographical regions. Other plant-growth-promoting abilities, such as phosphate solubilization and antifungal activity, have also been reported for this bacterium. With an aim at investigating the potential use of P. tropica as an inoculant for improving the performance of wheat crop, in this work we evaluated an experimental inoculant formulated with P. tropica MTo-293 with respect to root colonization, the practical aspects of its application, and the effects under field conditions when applied to wheat seeds. Bacterial colonization was monitored by culture dependent techniques and the wheat yield determined by quantifying the total grain production in two different seasons. Rhizoplane and endophytic colonization in wheat roots was achieved efficiently (on average, 8 and 4 log colony-forming units/g fresh weight, respectively) even at relatively low concentrations of viable bacteria in the inoculum under controlled conditions. P. tropica was compatible with a widely used fungicide, maintained viability for 48 h once applied to seeds, and was also able to colonize wheat roots efficiently. Furthermore, we were able to formulate an inoculant that maintained bacterial viability for relatively long time periods. Preliminary field assays were realized, and even though the average yields values for the inoculated treatments remained above the uninoculated ones, no significant effects of inoculation were detected with or without fertilization. The correct physiologic behavior of P. tropica suggests the necessity to continue with field experiments under different conditions. |
| format | Artículo |
| id | INTA2932 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA29322018-07-31T15:42:56Z Assessment of bacterial inoculant formulated with Paraburkholderia tropica to enhance wheat productivity Bernabeu, Pamela Romina Garcia, Sabrina Soledad López, Ana Clara Vio, Santiago Adolfo Carrasco, Natalia Boiardi, Jose Luis Luna, Maria Flavia Bacteria Fijadora del Nitrógeno Endofitas Tratamiento de Semillas Trigo Nitrogen Fixing Bacteria Endophytes Seed Treatment Wheat Paraburkholderia tropica Paraburkholderia tropica is an endophytic nitrogen-fixing bacterium isolated from the rhizosphere, rhizoplane, and internal tissues of sugarcane and corn plants in different geographical regions. Other plant-growth-promoting abilities, such as phosphate solubilization and antifungal activity, have also been reported for this bacterium. With an aim at investigating the potential use of P. tropica as an inoculant for improving the performance of wheat crop, in this work we evaluated an experimental inoculant formulated with P. tropica MTo-293 with respect to root colonization, the practical aspects of its application, and the effects under field conditions when applied to wheat seeds. Bacterial colonization was monitored by culture dependent techniques and the wheat yield determined by quantifying the total grain production in two different seasons. Rhizoplane and endophytic colonization in wheat roots was achieved efficiently (on average, 8 and 4 log colony-forming units/g fresh weight, respectively) even at relatively low concentrations of viable bacteria in the inoculum under controlled conditions. P. tropica was compatible with a widely used fungicide, maintained viability for 48 h once applied to seeds, and was also able to colonize wheat roots efficiently. Furthermore, we were able to formulate an inoculant that maintained bacterial viability for relatively long time periods. Preliminary field assays were realized, and even though the average yields values for the inoculated treatments remained above the uninoculated ones, no significant effects of inoculation were detected with or without fertilization. The correct physiologic behavior of P. tropica suggests the necessity to continue with field experiments under different conditions. EEA Barrow Fil: Bernabeu, Pamela Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina Fil: Garcia, Sabrina Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina Fil: López, Ana Clara. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Biotecnología Misiones. Laboratorio de Biotecnología Molecular; Argentina Fil: Vio, Santiago Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina Fil: Carrasco, Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Barrow; Argentina Fil: Boiardi, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina. Universidad Tecnológica Nacional. Facultad Regional Buenos Aires; Argentina Fil: Luna, Maria Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina 2018-07-31T15:41:11Z 2018-07-31T15:41:11Z 2018-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007/s11274-018-2461-4 http://hdl.handle.net/20.500.12123/2932 0959-3993 1573-0972 https://doi.org/10.1007/s11274-018-2461-4 eng info:eu-repo/semantics/restrictedAccess application/pdf World Journal of Microbiology and Biotechnology 34 : 81 (June 2018) |
| spellingShingle | Bacteria Fijadora del Nitrógeno Endofitas Tratamiento de Semillas Trigo Nitrogen Fixing Bacteria Endophytes Seed Treatment Wheat Paraburkholderia tropica Bernabeu, Pamela Romina Garcia, Sabrina Soledad López, Ana Clara Vio, Santiago Adolfo Carrasco, Natalia Boiardi, Jose Luis Luna, Maria Flavia Assessment of bacterial inoculant formulated with Paraburkholderia tropica to enhance wheat productivity |
| title | Assessment of bacterial inoculant formulated with Paraburkholderia tropica to enhance wheat productivity |
| title_full | Assessment of bacterial inoculant formulated with Paraburkholderia tropica to enhance wheat productivity |
| title_fullStr | Assessment of bacterial inoculant formulated with Paraburkholderia tropica to enhance wheat productivity |
| title_full_unstemmed | Assessment of bacterial inoculant formulated with Paraburkholderia tropica to enhance wheat productivity |
| title_short | Assessment of bacterial inoculant formulated with Paraburkholderia tropica to enhance wheat productivity |
| title_sort | assessment of bacterial inoculant formulated with paraburkholderia tropica to enhance wheat productivity |
| topic | Bacteria Fijadora del Nitrógeno Endofitas Tratamiento de Semillas Trigo Nitrogen Fixing Bacteria Endophytes Seed Treatment Wheat Paraburkholderia tropica |
| url | https://link.springer.com/article/10.1007/s11274-018-2461-4 http://hdl.handle.net/20.500.12123/2932 https://doi.org/10.1007/s11274-018-2461-4 |
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