Determination of glyphosate, AMPA and glufosinate in dairy farm water from Argentina using a simplified UHPLC-MS/MS method
Argentina, together with the USA and Brazil, produces approximately 80% of the total worldwide glyphosate loadings. The development of a simplified ultra-high performance liquid chromatographic tandem mass spectrometric method (UHPLC-MS/MS) for the determination of glyphosate, aminomethylphosphonic...
| Autores principales: | , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S0048969718324161 http://hdl.handle.net/20.500.12123/2915 https://doi.org/10.1016/j.scitotenv.2018.06.340 |
| _version_ | 1855035050126475264 |
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| author | Demonte, Luisina Delma Michlig, Nicolás Gaggiotti, Monica Del Carmen Adam, Claudia Guadalupe Beldoménico, Horacio Ramón Repetti, María Rosa |
| author_browse | Adam, Claudia Guadalupe Beldoménico, Horacio Ramón Demonte, Luisina Delma Gaggiotti, Monica Del Carmen Michlig, Nicolás Repetti, María Rosa |
| author_facet | Demonte, Luisina Delma Michlig, Nicolás Gaggiotti, Monica Del Carmen Adam, Claudia Guadalupe Beldoménico, Horacio Ramón Repetti, María Rosa |
| author_sort | Demonte, Luisina Delma |
| collection | INTA Digital |
| description | Argentina, together with the USA and Brazil, produces approximately 80% of the total worldwide glyphosate loadings. The development of a simplified ultra-high performance liquid chromatographic tandem mass spectrometric method (UHPLC-MS/MS) for the determination of glyphosate, aminomethylphosphonic acid (AMPA) and glufosinate in water is described, including studies of several alternatives of 9-fluorenylmethylchloroformate (FMOC-Cl) derivatization and pretreatment steps. The proposed method includes acidification and neutralization of a low sample volume (3 mL), 2 hours derivatization step, cleanup with dichloromethane, followed by reverse phase UHPLC-MS/MS determination of the analytes. Figures of merit were satisfactory in terms of linearity, selectivity, accuracy and intermediate precision (%REC 70–105% with RSD < 15%). Limits of quantification (LOQ) were suitable for monitoring purposes (0.6, 0.2, 0.1 μg/L for glyphosate, AMPA and glufosinate respectively). The validated methodology was applied for the analysis of livestock wells waters from 40 dairy farms located in the central region of Argentina. Glyphosate and AMPA were quantified in 15% and 53% of the analyzed samples with concentrations ranging from 0.6–11.3 μg/L and 0.2–6.5 μg/L respectively. Greater concentrations of glyphosate were also verified in waters from open-reservoir tanks, which are directly exposed to the farm environment. In these cases glyphosate and AMPA occurrence increased, being quantified in the 33% and 61% of the samples with values ranging 0.6–21.2 μg/L and 0.2–4.2 μg/L respectively. Also in this case glufosinate was found in 52% samples at <LOQ levels and was quantified in one sample at 0.1 μg/L. This new information constitutes an important contribution to authorities and scientists for further research, control and risk analysis purposes. |
| format | info:ar-repo/semantics/artículo |
| id | INTA2915 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA29152018-07-30T14:17:42Z Determination of glyphosate, AMPA and glufosinate in dairy farm water from Argentina using a simplified UHPLC-MS/MS method Demonte, Luisina Delma Michlig, Nicolás Gaggiotti, Monica Del Carmen Adam, Claudia Guadalupe Beldoménico, Horacio Ramón Repetti, María Rosa Granjas Lecheras Agua Glifosato Glufosinato Experimentación Dairy Farms Water Glyphosate Glufosinate Experimentation Argentina Tambos Argentina, together with the USA and Brazil, produces approximately 80% of the total worldwide glyphosate loadings. The development of a simplified ultra-high performance liquid chromatographic tandem mass spectrometric method (UHPLC-MS/MS) for the determination of glyphosate, aminomethylphosphonic acid (AMPA) and glufosinate in water is described, including studies of several alternatives of 9-fluorenylmethylchloroformate (FMOC-Cl) derivatization and pretreatment steps. The proposed method includes acidification and neutralization of a low sample volume (3 mL), 2 hours derivatization step, cleanup with dichloromethane, followed by reverse phase UHPLC-MS/MS determination of the analytes. Figures of merit were satisfactory in terms of linearity, selectivity, accuracy and intermediate precision (%REC 70–105% with RSD < 15%). Limits of quantification (LOQ) were suitable for monitoring purposes (0.6, 0.2, 0.1 μg/L for glyphosate, AMPA and glufosinate respectively). The validated methodology was applied for the analysis of livestock wells waters from 40 dairy farms located in the central region of Argentina. Glyphosate and AMPA were quantified in 15% and 53% of the analyzed samples with concentrations ranging from 0.6–11.3 μg/L and 0.2–6.5 μg/L respectively. Greater concentrations of glyphosate were also verified in waters from open-reservoir tanks, which are directly exposed to the farm environment. In these cases glyphosate and AMPA occurrence increased, being quantified in the 33% and 61% of the samples with values ranging 0.6–21.2 μg/L and 0.2–4.2 μg/L respectively. Also in this case glufosinate was found in 52% samples at <LOQ levels and was quantified in one sample at 0.1 μg/L. This new information constitutes an important contribution to authorities and scientists for further research, control and risk analysis purposes. EEA Rafaela Fil: Demonte, Luisina Delma. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos (PRINARC); Argentina Fil: Michlig, Nicolás. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos (PRINARC); Argentina Fil: Gaggiotti, Monica Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Adam, Claudia Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. IQAL; Argentina Fil: Beldoménico, Horacio Ramón. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos (PRINARC); Argentina Fil: Repetti, María Rosa. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos (PRINARC); Argentina 2018-07-30T14:16:12Z 2018-07-30T14:16:12Z 2018-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0048969718324161 http://hdl.handle.net/20.500.12123/2915 0048-9697 1879-1026 https://doi.org/10.1016/j.scitotenv.2018.06.340 eng info:eu-repo/semantics/restrictedAccess application/pdf Science of The Total Environment 645 : 34-43 (December 2018) In Progress |
| spellingShingle | Granjas Lecheras Agua Glifosato Glufosinato Experimentación Dairy Farms Water Glyphosate Glufosinate Experimentation Argentina Tambos Demonte, Luisina Delma Michlig, Nicolás Gaggiotti, Monica Del Carmen Adam, Claudia Guadalupe Beldoménico, Horacio Ramón Repetti, María Rosa Determination of glyphosate, AMPA and glufosinate in dairy farm water from Argentina using a simplified UHPLC-MS/MS method |
| title | Determination of glyphosate, AMPA and glufosinate in dairy farm water from Argentina using a simplified UHPLC-MS/MS method |
| title_full | Determination of glyphosate, AMPA and glufosinate in dairy farm water from Argentina using a simplified UHPLC-MS/MS method |
| title_fullStr | Determination of glyphosate, AMPA and glufosinate in dairy farm water from Argentina using a simplified UHPLC-MS/MS method |
| title_full_unstemmed | Determination of glyphosate, AMPA and glufosinate in dairy farm water from Argentina using a simplified UHPLC-MS/MS method |
| title_short | Determination of glyphosate, AMPA and glufosinate in dairy farm water from Argentina using a simplified UHPLC-MS/MS method |
| title_sort | determination of glyphosate ampa and glufosinate in dairy farm water from argentina using a simplified uhplc ms ms method |
| topic | Granjas Lecheras Agua Glifosato Glufosinato Experimentación Dairy Farms Water Glyphosate Glufosinate Experimentation Argentina Tambos |
| url | https://www.sciencedirect.com/science/article/pii/S0048969718324161 http://hdl.handle.net/20.500.12123/2915 https://doi.org/10.1016/j.scitotenv.2018.06.340 |
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