Adjustment of a rapid method for quantification of Fusarium spp. spore suspensions in plant pathology = Ajuste de un método rápido para la cuantificación de suspensiones de esporas de Fusarium spp. en fitopatología
The use of a Neubauer chamber is a broadly employed method when cell suspensions need to be quantified. However, this technique may take a long time and needs trained personnel. Spectrophotometry has proved to be a rapid, simple and accurate method to estimate the concentration of spore suspensions...
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| Format: | Artículo |
| Language: | Inglés |
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2018
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| Online Access: | https://www.sciencedirect.com/science/article/pii/S0325754115000449 http://hdl.handle.net/20.500.12123/2909 https://doi.org/10.1016/j.ram.2015.03.002 |
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| author | Caligiore Gei, Pablo Fernando Valdez, Jorge Gustavo |
| author_browse | Caligiore Gei, Pablo Fernando Valdez, Jorge Gustavo |
| author_facet | Caligiore Gei, Pablo Fernando Valdez, Jorge Gustavo |
| author_sort | Caligiore Gei, Pablo Fernando |
| collection | INTA Digital |
| description | The use of a Neubauer chamber is a broadly employed method when cell suspensions need to be quantified. However, this technique may take a long time and needs trained personnel. Spectrophotometry has proved to be a rapid, simple and accurate method to estimate the concentration of spore suspensions of isolates of the genus Fusarium. In this work we present a linear formula to relate absorbance measurements at 530 nm with the number of microconidia/ml in a suspension. |
| format | Artículo |
| id | INTA2909 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA29092018-07-30T12:25:17Z Adjustment of a rapid method for quantification of Fusarium spp. spore suspensions in plant pathology = Ajuste de un método rápido para la cuantificación de suspensiones de esporas de Fusarium spp. en fitopatología Caligiore Gei, Pablo Fernando Valdez, Jorge Gustavo Fusarium Hongos Esporas Fitopatología Análisis Cuantitativo Fungi Spores Plant Pathology Quantitative Analysis The use of a Neubauer chamber is a broadly employed method when cell suspensions need to be quantified. However, this technique may take a long time and needs trained personnel. Spectrophotometry has proved to be a rapid, simple and accurate method to estimate the concentration of spore suspensions of isolates of the genus Fusarium. In this work we present a linear formula to relate absorbance measurements at 530 nm with the number of microconidia/ml in a suspension. La cámara de Neubauer es un método ampliamente utilizado para cuantificar suspensiones de esporas. Sin embargo, esta técnica requiere personal entrenado y un tiempo considerable. La espectrofotometría demostró ser un método rápido, simple y preciso para estimar la concentración de esporas de aislados del género Fusarium. En este artículo breve presentamos una fórmula lineal ajustada para relacionar la absorbancia a 530 nm con el número de conidios en suspensiones utilizadas en pruebas fitopatológicas. EEA La Consulta Fil: Caligiore Gei, Pablo Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina Fil: Valdez, Jorge Gustavo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina 2018-07-30T12:23:23Z 2018-07-30T12:23:23Z 2015-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0325754115000449 http://hdl.handle.net/20.500.12123/2909 0325-7541 1851-7617 https://doi.org/10.1016/j.ram.2015.03.002 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Revista Argentina de Microbiología 47 (2) : 152-154 (April–June 2015) |
| spellingShingle | Fusarium Hongos Esporas Fitopatología Análisis Cuantitativo Fungi Spores Plant Pathology Quantitative Analysis Caligiore Gei, Pablo Fernando Valdez, Jorge Gustavo Adjustment of a rapid method for quantification of Fusarium spp. spore suspensions in plant pathology = Ajuste de un método rápido para la cuantificación de suspensiones de esporas de Fusarium spp. en fitopatología |
| title | Adjustment of a rapid method for quantification of Fusarium spp. spore suspensions in plant pathology = Ajuste de un método rápido para la cuantificación de suspensiones de esporas de Fusarium spp. en fitopatología |
| title_full | Adjustment of a rapid method for quantification of Fusarium spp. spore suspensions in plant pathology = Ajuste de un método rápido para la cuantificación de suspensiones de esporas de Fusarium spp. en fitopatología |
| title_fullStr | Adjustment of a rapid method for quantification of Fusarium spp. spore suspensions in plant pathology = Ajuste de un método rápido para la cuantificación de suspensiones de esporas de Fusarium spp. en fitopatología |
| title_full_unstemmed | Adjustment of a rapid method for quantification of Fusarium spp. spore suspensions in plant pathology = Ajuste de un método rápido para la cuantificación de suspensiones de esporas de Fusarium spp. en fitopatología |
| title_short | Adjustment of a rapid method for quantification of Fusarium spp. spore suspensions in plant pathology = Ajuste de un método rápido para la cuantificación de suspensiones de esporas de Fusarium spp. en fitopatología |
| title_sort | adjustment of a rapid method for quantification of fusarium spp spore suspensions in plant pathology ajuste de un metodo rapido para la cuantificacion de suspensiones de esporas de fusarium spp en fitopatologia |
| topic | Fusarium Hongos Esporas Fitopatología Análisis Cuantitativo Fungi Spores Plant Pathology Quantitative Analysis |
| url | https://www.sciencedirect.com/science/article/pii/S0325754115000449 http://hdl.handle.net/20.500.12123/2909 https://doi.org/10.1016/j.ram.2015.03.002 |
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