IgY against enterotoxigenic Escherichia coli administered by hydrogel-carbon nanotubes composites to prevent neonatal diarrhoea in experimentally challenged piglets
In previous studies, the applicability of polymeric hydrogels for the protection of egg yolk immunoglobulin (IgY) against simulated gastric conditions was established. Thereafter, the performance of the hydrogels was improved with the addition of chitosan wrapped carbon nanotubes and the in vitro to...
| Autores principales: | , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S0264410X16302766?via%3Dihub http://hdl.handle.net/20.500.12123/2904 https://doi.org/10.1016/j.vaccine.2016.05.004 |
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| author | Alustiza, Fabrisio Eduardo Bellingeri, Romina Picco, Natalia Motta, Carlos Grosso, M. Carolina Barbero, Cesar A. Acevedo, Diego F. Vivas, Adriana |
| author_browse | Acevedo, Diego F. Alustiza, Fabrisio Eduardo Barbero, Cesar A. Bellingeri, Romina Grosso, M. Carolina Motta, Carlos Picco, Natalia Vivas, Adriana |
| author_facet | Alustiza, Fabrisio Eduardo Bellingeri, Romina Picco, Natalia Motta, Carlos Grosso, M. Carolina Barbero, Cesar A. Acevedo, Diego F. Vivas, Adriana |
| author_sort | Alustiza, Fabrisio Eduardo |
| collection | INTA Digital |
| description | In previous studies, the applicability of polymeric hydrogels for the protection of egg yolk immunoglobulin (IgY) against simulated gastric conditions was established. Thereafter, the performance of the hydrogels was improved with the addition of chitosan wrapped carbon nanotubes and the in vitro toxicity for porcine intestinal cells of these nanocomposites was assessed. The objective of the present study was to evaluate in vivo the protective efficacy of the nanocomoposite matrix for IgY when the immunoglobulin is used against enterotoxigenic Escherichia coli (ETEC) in challenged piglets. Groups of piglets orally challenged with 1011 CFU/mL of ETEC were treated with non-protected and protected IgY. The clinical response of each group was monitored and evaluated in terms of dehydration, rectal temperature, faecal consistency score and body weight gain. Blood parameters and histological aspects were also studied. The results showed that treatment of infected piglets with protected IgY reduced significantly the severity of diarrhea. Non-protected IgY group show a lower recovery rate. Blood parameters and histological aspects were normal in both groups. Collectively, these results support previous in vitro studies showing that the nanocomposites can be an effective method of IgY protection against gastric inactivation. |
| format | Artículo |
| id | INTA2904 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA29042018-07-27T14:56:57Z IgY against enterotoxigenic Escherichia coli administered by hydrogel-carbon nanotubes composites to prevent neonatal diarrhoea in experimentally challenged piglets Alustiza, Fabrisio Eduardo Bellingeri, Romina Picco, Natalia Motta, Carlos Grosso, M. Carolina Barbero, Cesar A. Acevedo, Diego F. Vivas, Adriana Inmunoglobulina Yema de Huevo Escherichia coli Geles Nanocompuestos Lechón Prevención de Enfermedades Diarrea Immunoglobulins Egg Yolk Gels Nanocomposites Piglets Disease Prevention Diarrhoea In previous studies, the applicability of polymeric hydrogels for the protection of egg yolk immunoglobulin (IgY) against simulated gastric conditions was established. Thereafter, the performance of the hydrogels was improved with the addition of chitosan wrapped carbon nanotubes and the in vitro toxicity for porcine intestinal cells of these nanocomposites was assessed. The objective of the present study was to evaluate in vivo the protective efficacy of the nanocomoposite matrix for IgY when the immunoglobulin is used against enterotoxigenic Escherichia coli (ETEC) in challenged piglets. Groups of piglets orally challenged with 1011 CFU/mL of ETEC were treated with non-protected and protected IgY. The clinical response of each group was monitored and evaluated in terms of dehydration, rectal temperature, faecal consistency score and body weight gain. Blood parameters and histological aspects were also studied. The results showed that treatment of infected piglets with protected IgY reduced significantly the severity of diarrhea. Non-protected IgY group show a lower recovery rate. Blood parameters and histological aspects were normal in both groups. Collectively, these results support previous in vitro studies showing that the nanocomposites can be an effective method of IgY protection against gastric inactivation. EEA Marcos Juárez Fil: Alustiza, Fabrisio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Bellingeri, Romina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal. Laboratorio de Biotecnología Animal; Argentina Fil: Picco, Natalia. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal. Laboratorio de Biotecnología Animal; Argentina Fil: Motta, Carlos. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Patología Animal; Argentina Fil: Grosso, M. Carolina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal. Laboratorio de Biotecnología Animal; Argentina Fil: Barbero, Cesar A.. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Química. Laboratorio de Materiales Avanzados; Argentina Fil: Acevedo, Diego F. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Química. Laboratorio de Materiales Avanzados; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ingeniería. Departamento de Tecnología Química; Argentina Fil: Vivas, Adriana. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal. Laboratorio de Biotecnología Animal; Argentina 2018-07-27T14:55:16Z 2018-07-27T14:55:16Z 2016-06-14 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0264410X16302766?via%3Dihub http://hdl.handle.net/20.500.12123/2904 0264-410X https://doi.org/10.1016/j.vaccine.2016.05.004 eng info:eu-repo/semantics/restrictedAccess application/pdf Vaccine 34 (28) : 3291-3297 (June 2016) |
| spellingShingle | Inmunoglobulina Yema de Huevo Escherichia coli Geles Nanocompuestos Lechón Prevención de Enfermedades Diarrea Immunoglobulins Egg Yolk Gels Nanocomposites Piglets Disease Prevention Diarrhoea Alustiza, Fabrisio Eduardo Bellingeri, Romina Picco, Natalia Motta, Carlos Grosso, M. Carolina Barbero, Cesar A. Acevedo, Diego F. Vivas, Adriana IgY against enterotoxigenic Escherichia coli administered by hydrogel-carbon nanotubes composites to prevent neonatal diarrhoea in experimentally challenged piglets |
| title | IgY against enterotoxigenic Escherichia coli administered by hydrogel-carbon nanotubes composites to prevent neonatal diarrhoea in experimentally challenged piglets |
| title_full | IgY against enterotoxigenic Escherichia coli administered by hydrogel-carbon nanotubes composites to prevent neonatal diarrhoea in experimentally challenged piglets |
| title_fullStr | IgY against enterotoxigenic Escherichia coli administered by hydrogel-carbon nanotubes composites to prevent neonatal diarrhoea in experimentally challenged piglets |
| title_full_unstemmed | IgY against enterotoxigenic Escherichia coli administered by hydrogel-carbon nanotubes composites to prevent neonatal diarrhoea in experimentally challenged piglets |
| title_short | IgY against enterotoxigenic Escherichia coli administered by hydrogel-carbon nanotubes composites to prevent neonatal diarrhoea in experimentally challenged piglets |
| title_sort | igy against enterotoxigenic escherichia coli administered by hydrogel carbon nanotubes composites to prevent neonatal diarrhoea in experimentally challenged piglets |
| topic | Inmunoglobulina Yema de Huevo Escherichia coli Geles Nanocompuestos Lechón Prevención de Enfermedades Diarrea Immunoglobulins Egg Yolk Gels Nanocomposites Piglets Disease Prevention Diarrhoea |
| url | https://www.sciencedirect.com/science/article/pii/S0264410X16302766?via%3Dihub http://hdl.handle.net/20.500.12123/2904 https://doi.org/10.1016/j.vaccine.2016.05.004 |
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