Transgene escape and persistence in an agroecosystem: the case of glyphosate-resistant Brassica rapa L. in central Argentina
Brassica rapa L. is an annual Brassicaceae species cultivated for oil and food production, whose wild form is a weed of crops worldwide. In temperate regions of South America and especially in the Argentine Pampas region, this species is widely distributed. During 2014, wild B. rapa populations that...
| Autores principales: | , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/2894 https://link.springer.com/article/10.1007/s11356-017-0726-3 https://doi.org/10.1007/s11356-017-0726-3 |
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| author | Pandolfo, Claudio Ezequiel Presotto, Alejandro Daniel Torres Carbonell, Francisco Javier Ureta, Maria Soledad Poverene, María Mónica Cantamutto, Miguel Angel |
| author_browse | Cantamutto, Miguel Angel Pandolfo, Claudio Ezequiel Poverene, María Mónica Presotto, Alejandro Daniel Torres Carbonell, Francisco Javier Ureta, Maria Soledad |
| author_facet | Pandolfo, Claudio Ezequiel Presotto, Alejandro Daniel Torres Carbonell, Francisco Javier Ureta, Maria Soledad Poverene, María Mónica Cantamutto, Miguel Angel |
| author_sort | Pandolfo, Claudio Ezequiel |
| collection | INTA Digital |
| description | Brassica rapa L. is an annual Brassicaceae species cultivated for oil and food production, whose wild form is a weed of crops worldwide. In temperate regions of South America and especially in the Argentine Pampas region, this species is widely distributed. During 2014, wild B. rapa populations that escaped control with glyphosate applications by farmers were found in this area. These plants were characterized by morphology and seed acidic profile, and all the characters agreed with B. rapa description. The dose-response assays showed that the biotypes were highly resistant to glyphosate. It was also shown that they had multiple resistance to AHAS-inhibiting herbicides. The transgenic origin of the glyphosate resistance in B. rapa biotypes was verified by an immunological test which confirmed the presence of the CP4 EPSPS protein and by an event-specific GT73 molecular marker. The persistence of the transgene in nature was confirmed for at least 4 years, in ruderal and agrestal habitats. This finding suggests that glyphosate resistance might come from GM oilseed rape crops illegally cultivated in the country or as a seed contaminant, and it implies gene flow and introgression between feral populations of GM B. napus and wild B. rapa. The persistence and spread of the resistance in agricultural environments was promoted by the high selection pressure imposed by intensive herbicide usage in the prevalent no-till farming systems. |
| format | info:ar-repo/semantics/artículo |
| id | INTA2894 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA28942024-04-30T12:09:14Z Transgene escape and persistence in an agroecosystem: the case of glyphosate-resistant Brassica rapa L. in central Argentina Pandolfo, Claudio Ezequiel Presotto, Alejandro Daniel Torres Carbonell, Francisco Javier Ureta, Maria Soledad Poverene, María Mónica Cantamutto, Miguel Angel Brassica campestris Malezas Herbicidas Glifosato Resistencia a los Herbicidas Agroecosistemas Transgenes Weeds Weed Control Herbicides Glyphosate Resistance to Herbicides Agroecosystems Argentina Brassica rapa Control de Malezas Brassica rapa L. is an annual Brassicaceae species cultivated for oil and food production, whose wild form is a weed of crops worldwide. In temperate regions of South America and especially in the Argentine Pampas region, this species is widely distributed. During 2014, wild B. rapa populations that escaped control with glyphosate applications by farmers were found in this area. These plants were characterized by morphology and seed acidic profile, and all the characters agreed with B. rapa description. The dose-response assays showed that the biotypes were highly resistant to glyphosate. It was also shown that they had multiple resistance to AHAS-inhibiting herbicides. The transgenic origin of the glyphosate resistance in B. rapa biotypes was verified by an immunological test which confirmed the presence of the CP4 EPSPS protein and by an event-specific GT73 molecular marker. The persistence of the transgene in nature was confirmed for at least 4 years, in ruderal and agrestal habitats. This finding suggests that glyphosate resistance might come from GM oilseed rape crops illegally cultivated in the country or as a seed contaminant, and it implies gene flow and introgression between feral populations of GM B. napus and wild B. rapa. The persistence and spread of the resistance in agricultural environments was promoted by the high selection pressure imposed by intensive herbicide usage in the prevalent no-till farming systems. EEA Hilario Ascasubi Fil: Pandolfo, Claudio Ezequiel. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina Fil: Presotto, Alejandro Daniel. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina Fil: Torres Carbonell, Francisco Javier. Universidad Nacional del Sur. Departamento de Agronomía; Argentina Fil: Ureta, Maria Soledad. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina Fil: Poverene, María Mónica. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina Fil: Cantamutto, Miguel Angel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Hilario Ascasubi; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina 2018-07-27T11:42:36Z 2018-07-27T11:42:36Z 2018-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/2894 https://link.springer.com/article/10.1007/s11356-017-0726-3 0944-1344 1614-7499 https://doi.org/10.1007/s11356-017-0726-3 eng info:eu-repo/semantics/restrictedAccess application/pdf Environmental Science and Pollution Research 25 (7) : 6251–6264 (March 2018) |
| spellingShingle | Brassica campestris Malezas Herbicidas Glifosato Resistencia a los Herbicidas Agroecosistemas Transgenes Weeds Weed Control Herbicides Glyphosate Resistance to Herbicides Agroecosystems Argentina Brassica rapa Control de Malezas Pandolfo, Claudio Ezequiel Presotto, Alejandro Daniel Torres Carbonell, Francisco Javier Ureta, Maria Soledad Poverene, María Mónica Cantamutto, Miguel Angel Transgene escape and persistence in an agroecosystem: the case of glyphosate-resistant Brassica rapa L. in central Argentina |
| title | Transgene escape and persistence in an agroecosystem: the case of glyphosate-resistant Brassica rapa L. in central Argentina |
| title_full | Transgene escape and persistence in an agroecosystem: the case of glyphosate-resistant Brassica rapa L. in central Argentina |
| title_fullStr | Transgene escape and persistence in an agroecosystem: the case of glyphosate-resistant Brassica rapa L. in central Argentina |
| title_full_unstemmed | Transgene escape and persistence in an agroecosystem: the case of glyphosate-resistant Brassica rapa L. in central Argentina |
| title_short | Transgene escape and persistence in an agroecosystem: the case of glyphosate-resistant Brassica rapa L. in central Argentina |
| title_sort | transgene escape and persistence in an agroecosystem the case of glyphosate resistant brassica rapa l in central argentina |
| topic | Brassica campestris Malezas Herbicidas Glifosato Resistencia a los Herbicidas Agroecosistemas Transgenes Weeds Weed Control Herbicides Glyphosate Resistance to Herbicides Agroecosystems Argentina Brassica rapa Control de Malezas |
| url | http://hdl.handle.net/20.500.12123/2894 https://link.springer.com/article/10.1007/s11356-017-0726-3 https://doi.org/10.1007/s11356-017-0726-3 |
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