A new argasid tick species (Acari: Argasidae) associated with the rock cavy, Kerodon rupestris Wied-Neuwied (Rodentia: Caviidae), in a semiarid region of Brazil
Background: The rock cavy Kerodon rupestris (Wied-Neuwied, 1820) is a rodent species endemic to northeastern Brazil. Earlier studies have associated the argasid tick Ornithodoros talaje (Guérin-Méneville, 1849) with rocky cavy; however, a recent study proposed that O. talaje is not established in...
| Autores principales: | , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
BioMed Central
2018
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/2887 https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-016-1796-7 https://doi.org/10.1186/s13071-016-1796-7 |
| _version_ | 1855035044828020736 |
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| author | Labruna, Marcelo B. Nava, Santiago Marcili, Arlei Barbieri, Amália Regina Mar Nunes, Pablo Henrique Horta, Mauricio Claudio Venzal, José Manuel |
| author_browse | Barbieri, Amália Regina Mar Horta, Mauricio Claudio Labruna, Marcelo B. Marcili, Arlei Nava, Santiago Nunes, Pablo Henrique Venzal, José Manuel |
| author_facet | Labruna, Marcelo B. Nava, Santiago Marcili, Arlei Barbieri, Amália Regina Mar Nunes, Pablo Henrique Horta, Mauricio Claudio Venzal, José Manuel |
| author_sort | Labruna, Marcelo B. |
| collection | INTA Digital |
| description | Background: The rock cavy Kerodon rupestris (Wied-Neuwied, 1820) is a rodent species endemic to northeastern
Brazil. Earlier studies have associated the argasid tick Ornithodoros talaje (Guérin-Méneville, 1849) with rocky cavy;
however, a recent study proposed that O. talaje is not established in Brazil, where previous reports of this species
were possibly misidentifications of closely related species, yet to be properly determined. Here, we describe a new
species of Ornithodoros Koch, 1844 associated with rock cavies in northeastern Brazil.
Methods: During 2012–2013, Ornithodoros ticks were collected from K. rupestris resting places in Paraíba State (PB)
and Piauí State (PI), northeastern Brazil. These ticks were brought alive to the laboratory, and used to form
two laboratory colonies (PB and PI ticks). Field-collected adults and laboratory-reared larvae were used for morphological
description through light and scanning electron microscopy. DNA sequences of the mitochondrial 16S rRNA gene were
generated from nymphal ticks and used to conduct phylogenetic analyses along with other Ornithodoros spp.
sequences from GenBank. Reproductive compatibility of crosses between PB and PI adult ticks was evaluated,
as well as analyses of hybrid ticks through larval morphology by a principal components analysis (PCA) and
DNA sequences of the second internal transcribed spacer (ITS2) region from adult ticks.
Results: Morphological analysis allowed recognizing these ticks as a new species, Ornithodoros rietcorreai n. sp. The
larva of O. rietcorreai is distinct from those of other Ornithodoros spp. by the combination of the following character
states: 14 pairs of dorsal setae, dorsal plate pyriform, hypostome with pointed apex and dental formula 3/3 anteriorly,
2/2 posteriorly, and anal valves with long and pointed leaf-shaped ends. There were a few larval morphological
differences between PB and PI ticks, and their mitochondrial 16S rDNA sequences diverged by 3.3 %. On the other
hand, cross-mating experiments showed that PB and PI ticks were reproductive compatible, indicating that they
represent a single species. Analyses of ITS2 sequences and PCA corroborated this assumption.
Conclusion: Ornithodoros rietcorreai is described as a new species associated with K. rupestris in Brazil, increasing the
Brazilian tick fauna to 70 species. |
| format | info:ar-repo/semantics/artículo |
| id | INTA2887 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | BioMed Central |
| publisherStr | BioMed Central |
| record_format | dspace |
| spelling | INTA28872018-07-26T15:16:52Z A new argasid tick species (Acari: Argasidae) associated with the rock cavy, Kerodon rupestris Wied-Neuwied (Rodentia: Caviidae), in a semiarid region of Brazil Labruna, Marcelo B. Nava, Santiago Marcili, Arlei Barbieri, Amália Regina Mar Nunes, Pablo Henrique Horta, Mauricio Claudio Venzal, José Manuel Morfología Metastigmata Especies Nuevas Zona Semiárida Argasidae Semiarid Zones New Species Morphology Ornithodoros Rietcorreai Kerodon Rupestris Brasil Mitochondrial 16S rDNA Garrapatas Background: The rock cavy Kerodon rupestris (Wied-Neuwied, 1820) is a rodent species endemic to northeastern Brazil. Earlier studies have associated the argasid tick Ornithodoros talaje (Guérin-Méneville, 1849) with rocky cavy; however, a recent study proposed that O. talaje is not established in Brazil, where previous reports of this species were possibly misidentifications of closely related species, yet to be properly determined. Here, we describe a new species of Ornithodoros Koch, 1844 associated with rock cavies in northeastern Brazil. Methods: During 2012–2013, Ornithodoros ticks were collected from K. rupestris resting places in Paraíba State (PB) and Piauí State (PI), northeastern Brazil. These ticks were brought alive to the laboratory, and used to form two laboratory colonies (PB and PI ticks). Field-collected adults and laboratory-reared larvae were used for morphological description through light and scanning electron microscopy. DNA sequences of the mitochondrial 16S rRNA gene were generated from nymphal ticks and used to conduct phylogenetic analyses along with other Ornithodoros spp. sequences from GenBank. Reproductive compatibility of crosses between PB and PI adult ticks was evaluated, as well as analyses of hybrid ticks through larval morphology by a principal components analysis (PCA) and DNA sequences of the second internal transcribed spacer (ITS2) region from adult ticks. Results: Morphological analysis allowed recognizing these ticks as a new species, Ornithodoros rietcorreai n. sp. The larva of O. rietcorreai is distinct from those of other Ornithodoros spp. by the combination of the following character states: 14 pairs of dorsal setae, dorsal plate pyriform, hypostome with pointed apex and dental formula 3/3 anteriorly, 2/2 posteriorly, and anal valves with long and pointed leaf-shaped ends. There were a few larval morphological differences between PB and PI ticks, and their mitochondrial 16S rDNA sequences diverged by 3.3 %. On the other hand, cross-mating experiments showed that PB and PI ticks were reproductive compatible, indicating that they represent a single species. Analyses of ITS2 sequences and PCA corroborated this assumption. Conclusion: Ornithodoros rietcorreai is described as a new species associated with K. rupestris in Brazil, increasing the Brazilian tick fauna to 70 species. EEA Rafaela Fil: Labruna, Marcelo B. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; Brasil Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Marcili, Arlei. Universidade Federal do ABC. Centro de Ciências Naturais e Humanas; Brasil. Universidade de Santo Amaro. Medicina Veterinária e Bem estar animal; Brasil Fil: Barbieri, Amália Regina Mar. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; Brasil Fil: Nunes, Pablo Henrique. Universidade Federal da Integração Latino-Americana. Instituto Latino-Americano de Ciências da Vida e da Natureza; Brasil Fil: Horta, Mauricio Claudio. Universidade Federal do Vale do São Francisco. Campus de Ciências Agrárias; Brasil Fil: Venzal, José Manuel. Universidad de la República. Facultad de Veterinaria. Laboratorio de Vectores y Enfermedades Transmitidas; Uruguay 2018-07-26T15:01:29Z 2018-07-26T15:01:29Z 2016 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/2887 https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-016-1796-7 1756-3305 https://doi.org/10.1186/s13071-016-1796-7 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Brazil (nation) BioMed Central Parasites & vectors 9 : 511. (2016) |
| spellingShingle | Morfología Metastigmata Especies Nuevas Zona Semiárida Argasidae Semiarid Zones New Species Morphology Ornithodoros Rietcorreai Kerodon Rupestris Brasil Mitochondrial 16S rDNA Garrapatas Labruna, Marcelo B. Nava, Santiago Marcili, Arlei Barbieri, Amália Regina Mar Nunes, Pablo Henrique Horta, Mauricio Claudio Venzal, José Manuel A new argasid tick species (Acari: Argasidae) associated with the rock cavy, Kerodon rupestris Wied-Neuwied (Rodentia: Caviidae), in a semiarid region of Brazil |
| title | A new argasid tick species (Acari: Argasidae) associated with the rock cavy, Kerodon rupestris Wied-Neuwied (Rodentia: Caviidae), in a semiarid region of Brazil |
| title_full | A new argasid tick species (Acari: Argasidae) associated with the rock cavy, Kerodon rupestris Wied-Neuwied (Rodentia: Caviidae), in a semiarid region of Brazil |
| title_fullStr | A new argasid tick species (Acari: Argasidae) associated with the rock cavy, Kerodon rupestris Wied-Neuwied (Rodentia: Caviidae), in a semiarid region of Brazil |
| title_full_unstemmed | A new argasid tick species (Acari: Argasidae) associated with the rock cavy, Kerodon rupestris Wied-Neuwied (Rodentia: Caviidae), in a semiarid region of Brazil |
| title_short | A new argasid tick species (Acari: Argasidae) associated with the rock cavy, Kerodon rupestris Wied-Neuwied (Rodentia: Caviidae), in a semiarid region of Brazil |
| title_sort | new argasid tick species acari argasidae associated with the rock cavy kerodon rupestris wied neuwied rodentia caviidae in a semiarid region of brazil |
| topic | Morfología Metastigmata Especies Nuevas Zona Semiárida Argasidae Semiarid Zones New Species Morphology Ornithodoros Rietcorreai Kerodon Rupestris Brasil Mitochondrial 16S rDNA Garrapatas |
| url | http://hdl.handle.net/20.500.12123/2887 https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-016-1796-7 https://doi.org/10.1186/s13071-016-1796-7 |
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