Prevalence, antimicrobial resistance profile and comparison of selective plating media for the isolation of Salmonella in backyard chickens from Entre Rios, Argentina
This study was conducted to estimate the apparent prevalence of Salmonella spp. in birds kept under backyard system in Entre Ríos, Argentina, and determine the performance of two selective plating media used for Salmonella isolation, and the antimicrobial resistance of the isolated. Also, the ass...
| Main Authors: | , , , , , |
|---|---|
| Format: | Artículo |
| Language: | Inglés |
| Published: |
2018
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/2849 https://onlinelibrary.wiley.com/doi/full/10.1111/zph.12415 https://doi.org/10.1111/zph.12415 |
| _version_ | 1855483131856945152 |
|---|---|
| author | Rodriguez, Francisco Pascal, Diego C. Pulido, Diego Osinalde, José Manuel Caffer, María Inés Bueno, Dante Javier |
| author_browse | Bueno, Dante Javier Caffer, María Inés Osinalde, José Manuel Pascal, Diego C. Pulido, Diego Rodriguez, Francisco |
| author_facet | Rodriguez, Francisco Pascal, Diego C. Pulido, Diego Osinalde, José Manuel Caffer, María Inés Bueno, Dante Javier |
| author_sort | Rodriguez, Francisco |
| collection | INTA Digital |
| description | This study was conducted to estimate the apparent prevalence of Salmonella spp. in
birds kept under backyard system in Entre Ríos, Argentina, and determine the performance
of two selective plating media used for Salmonella isolation, and the antimicrobial
resistance of the isolated. Also, the association of farms characteristics with
Salmonella presence was evaluated. A total of 657 backyard chickens and 15 gooses
were sampled one time by cloacal swab, belonging to 51 and one family farms, respectively,
and four counties in Entre Rios state from April 2014 to May 2015. Only four
samples from backyard chickens belonged to three family farms from Uruguay County
were positive to Salmonella spp., so the apparent prevalence was 0.6% for this kind of
chicken. Four serovars were isolated (Salmonella ser. Lille, S. ser. Newport, S. ser.
Enteritidis and S. ser. Rissen), which were susceptible to all antibiotics tested with the
exception of erythromycin. For Hektoen enteric agar and brilliant green agar, relative
specificity and positive predictive value were 1, and the relative sensitivity and negative
predictive value did not show any difference between them. The agreement was
very good between these two plating media. None of the variables studied could be
selected to calculate the risk factors associated with Salmonella isolation because
p > .15. Although the prevalence of Salmonella spp. is low in backyard birds in Entre
Rios, the presence of S. ser. Enteritidis should not be discounted, because it is found in
the county that concentrates a large population of intensive poultry production in the
state. |
| format | Artículo |
| id | INTA2849 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA28492024-04-04T13:47:11Z Prevalence, antimicrobial resistance profile and comparison of selective plating media for the isolation of Salmonella in backyard chickens from Entre Rios, Argentina Rodriguez, Francisco Pascal, Diego C. Pulido, Diego Osinalde, José Manuel Caffer, María Inés Bueno, Dante Javier Pollo Salmonella Cultivo de Células Morbosidad Resistencia a los Antibióticos Resistance to Antibiotics Chickens Cell Culture Backyard Chicken Entre Ríos This study was conducted to estimate the apparent prevalence of Salmonella spp. in birds kept under backyard system in Entre Ríos, Argentina, and determine the performance of two selective plating media used for Salmonella isolation, and the antimicrobial resistance of the isolated. Also, the association of farms characteristics with Salmonella presence was evaluated. A total of 657 backyard chickens and 15 gooses were sampled one time by cloacal swab, belonging to 51 and one family farms, respectively, and four counties in Entre Rios state from April 2014 to May 2015. Only four samples from backyard chickens belonged to three family farms from Uruguay County were positive to Salmonella spp., so the apparent prevalence was 0.6% for this kind of chicken. Four serovars were isolated (Salmonella ser. Lille, S. ser. Newport, S. ser. Enteritidis and S. ser. Rissen), which were susceptible to all antibiotics tested with the exception of erythromycin. For Hektoen enteric agar and brilliant green agar, relative specificity and positive predictive value were 1, and the relative sensitivity and negative predictive value did not show any difference between them. The agreement was very good between these two plating media. None of the variables studied could be selected to calculate the risk factors associated with Salmonella isolation because p > .15. Although the prevalence of Salmonella spp. is low in backyard birds in Entre Rios, the presence of S. ser. Enteritidis should not be discounted, because it is found in the county that concentrates a large population of intensive poultry production in the state. EEA Concepción del Uruguay Fil: Rodríguez, Francisco Isabelino. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pascal, Diego C. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología, Sede Basavilbaso; Argentina Fil: Pulido, Diego. Fundación ArgenINTA, Paraná; Argentina Fil: Osinalde, José M. Ministerio de Producción de Entre Ríos. Dirección General de Recursos Naturales; Argentina Fil: Caffer, María Inés. Instituto Nacional de Enfermedades Infecciosas (INEI) - ANLIS “Dr Carlos G. Malbrán”. Servicio de Enterobacterias; Argentina Fil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología, Sede Basavilbaso ; Argentina 2018-07-23T12:52:39Z 2018-07-23T12:52:39Z 2018-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/2849 https://onlinelibrary.wiley.com/doi/full/10.1111/zph.12415 1863-2378 https://doi.org/10.1111/zph.12415 eng info:eu-repograntAgreement/INTA/PNSA/1115056/AR./Enfermedades infecciosas de las aves. info:eu-repo/semantics/restrictedAccess application/pdf Entre Ríos (province) Argentina (nation) Zoonoses public health 65 (1) : e95-e101. (February 2018) |
| spellingShingle | Pollo Salmonella Cultivo de Células Morbosidad Resistencia a los Antibióticos Resistance to Antibiotics Chickens Cell Culture Backyard Chicken Entre Ríos Rodriguez, Francisco Pascal, Diego C. Pulido, Diego Osinalde, José Manuel Caffer, María Inés Bueno, Dante Javier Prevalence, antimicrobial resistance profile and comparison of selective plating media for the isolation of Salmonella in backyard chickens from Entre Rios, Argentina |
| title | Prevalence, antimicrobial resistance profile and comparison of selective plating media for the isolation of Salmonella in backyard chickens from Entre Rios, Argentina |
| title_full | Prevalence, antimicrobial resistance profile and comparison of selective plating media for the isolation of Salmonella in backyard chickens from Entre Rios, Argentina |
| title_fullStr | Prevalence, antimicrobial resistance profile and comparison of selective plating media for the isolation of Salmonella in backyard chickens from Entre Rios, Argentina |
| title_full_unstemmed | Prevalence, antimicrobial resistance profile and comparison of selective plating media for the isolation of Salmonella in backyard chickens from Entre Rios, Argentina |
| title_short | Prevalence, antimicrobial resistance profile and comparison of selective plating media for the isolation of Salmonella in backyard chickens from Entre Rios, Argentina |
| title_sort | prevalence antimicrobial resistance profile and comparison of selective plating media for the isolation of salmonella in backyard chickens from entre rios argentina |
| topic | Pollo Salmonella Cultivo de Células Morbosidad Resistencia a los Antibióticos Resistance to Antibiotics Chickens Cell Culture Backyard Chicken Entre Ríos |
| url | http://hdl.handle.net/20.500.12123/2849 https://onlinelibrary.wiley.com/doi/full/10.1111/zph.12415 https://doi.org/10.1111/zph.12415 |
| work_keys_str_mv | AT rodriguezfrancisco prevalenceantimicrobialresistanceprofileandcomparisonofselectiveplatingmediafortheisolationofsalmonellainbackyardchickensfromentreriosargentina AT pascaldiegoc prevalenceantimicrobialresistanceprofileandcomparisonofselectiveplatingmediafortheisolationofsalmonellainbackyardchickensfromentreriosargentina AT pulidodiego prevalenceantimicrobialresistanceprofileandcomparisonofselectiveplatingmediafortheisolationofsalmonellainbackyardchickensfromentreriosargentina AT osinaldejosemanuel prevalenceantimicrobialresistanceprofileandcomparisonofselectiveplatingmediafortheisolationofsalmonellainbackyardchickensfromentreriosargentina AT caffermariaines prevalenceantimicrobialresistanceprofileandcomparisonofselectiveplatingmediafortheisolationofsalmonellainbackyardchickensfromentreriosargentina AT buenodantejavier prevalenceantimicrobialresistanceprofileandcomparisonofselectiveplatingmediafortheisolationofsalmonellainbackyardchickensfromentreriosargentina |