Citrus psorosis virus coat protein-derived hairpin construct confers stable transgenic resistance in citrus against psorosis A and B syndromes
Citrus psorosis virus (CPsV) is the causal agent of psorosis, a serious and widespread citrus disease. Two syndromes of psorosis, PsA and PsB, have been described. PsB is the most aggressive and rampant form. Previously, we obtained Pineapple sweet orange plants transformed with a hairpin construct...
| Autores principales: | , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
|
| Materias: | |
| Acceso en línea: | https://link.springer.com/article/10.1007%2Fs11248-016-0001-2 http://hdl.handle.net/20.500.12123/2843 https://doi.org/10.1007/s11248-016-0001-2 |
| _version_ | 1855483130706657280 |
|---|---|
| author | de Francesco, Agustina Costa, Norma Beatriz Garcia, Maria Laura |
| author_browse | Costa, Norma Beatriz Garcia, Maria Laura de Francesco, Agustina |
| author_facet | de Francesco, Agustina Costa, Norma Beatriz Garcia, Maria Laura |
| author_sort | de Francesco, Agustina |
| collection | INTA Digital |
| description | Citrus psorosis virus (CPsV) is the causal agent of psorosis, a serious and widespread citrus disease. Two syndromes of psorosis, PsA and PsB, have been described. PsB is the most aggressive and rampant form. Previously, we obtained Pineapple sweet orange plants transformed with a hairpin construct derived from the CPsV coat protein gene (ihpCP). Some of these plants were resistant to CPsV 90-1-1, a PsA isolate homologous to the transgene. In this study, we found that expression of the ihpCP transgene and siRNA production in lines ihpCP-10 and -15 were stable with time and propagation. In particular, line ihpCP-15 has been resistant for more than 2 years, even after re-inoculation. The ihpCP plants were also resistant against a heterologous CPsV isolate that causes severe PsB syndrome. Line ihpCP-15 manifested complete resistance while line ihpCP-10 was tolerant to the virus, although with variable behaviour, showing delay and attenuation in PsB symptoms. These lines are promising for a biotech product aimed at eradicating psorosis. |
| format | Artículo |
| id | INTA2843 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA28432018-07-20T15:55:58Z Citrus psorosis virus coat protein-derived hairpin construct confers stable transgenic resistance in citrus against psorosis A and B syndromes de Francesco, Agustina Costa, Norma Beatriz Garcia, Maria Laura Citrus Virus de las Plantas Plantas Transgénicas Resistencia a la Enfermedad Plant Viruses Transgenic Plants Disease Resistance Citrus Psorosis Virus Virus de la Psoriasis de los Cítricos Citrus psorosis virus (CPsV) is the causal agent of psorosis, a serious and widespread citrus disease. Two syndromes of psorosis, PsA and PsB, have been described. PsB is the most aggressive and rampant form. Previously, we obtained Pineapple sweet orange plants transformed with a hairpin construct derived from the CPsV coat protein gene (ihpCP). Some of these plants were resistant to CPsV 90-1-1, a PsA isolate homologous to the transgene. In this study, we found that expression of the ihpCP transgene and siRNA production in lines ihpCP-10 and -15 were stable with time and propagation. In particular, line ihpCP-15 has been resistant for more than 2 years, even after re-inoculation. The ihpCP plants were also resistant against a heterologous CPsV isolate that causes severe PsB syndrome. Line ihpCP-15 manifested complete resistance while line ihpCP-10 was tolerant to the virus, although with variable behaviour, showing delay and attenuation in PsB symptoms. These lines are promising for a biotech product aimed at eradicating psorosis. EEA Concordia Fil: de Francesco, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Costa, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Garcia, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina 2018-07-20T15:44:03Z 2018-07-20T15:44:03Z 2017-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007%2Fs11248-016-0001-2 http://hdl.handle.net/20.500.12123/2843 0962-8819 1573-9368 https://doi.org/10.1007/s11248-016-0001-2 eng info:eu-repo/semantics/restrictedAccess application/pdf Transgenic Research 26 (2) : 225–235 (April 2017) |
| spellingShingle | Citrus Virus de las Plantas Plantas Transgénicas Resistencia a la Enfermedad Plant Viruses Transgenic Plants Disease Resistance Citrus Psorosis Virus Virus de la Psoriasis de los Cítricos de Francesco, Agustina Costa, Norma Beatriz Garcia, Maria Laura Citrus psorosis virus coat protein-derived hairpin construct confers stable transgenic resistance in citrus against psorosis A and B syndromes |
| title | Citrus psorosis virus coat protein-derived hairpin construct confers stable transgenic resistance in citrus against psorosis A and B syndromes |
| title_full | Citrus psorosis virus coat protein-derived hairpin construct confers stable transgenic resistance in citrus against psorosis A and B syndromes |
| title_fullStr | Citrus psorosis virus coat protein-derived hairpin construct confers stable transgenic resistance in citrus against psorosis A and B syndromes |
| title_full_unstemmed | Citrus psorosis virus coat protein-derived hairpin construct confers stable transgenic resistance in citrus against psorosis A and B syndromes |
| title_short | Citrus psorosis virus coat protein-derived hairpin construct confers stable transgenic resistance in citrus against psorosis A and B syndromes |
| title_sort | citrus psorosis virus coat protein derived hairpin construct confers stable transgenic resistance in citrus against psorosis a and b syndromes |
| topic | Citrus Virus de las Plantas Plantas Transgénicas Resistencia a la Enfermedad Plant Viruses Transgenic Plants Disease Resistance Citrus Psorosis Virus Virus de la Psoriasis de los Cítricos |
| url | https://link.springer.com/article/10.1007%2Fs11248-016-0001-2 http://hdl.handle.net/20.500.12123/2843 https://doi.org/10.1007/s11248-016-0001-2 |
| work_keys_str_mv | AT defrancescoagustina citruspsorosisviruscoatproteinderivedhairpinconstructconfersstabletransgenicresistanceincitrusagainstpsorosisaandbsyndromes AT costanormabeatriz citruspsorosisviruscoatproteinderivedhairpinconstructconfersstabletransgenicresistanceincitrusagainstpsorosisaandbsyndromes AT garciamarialaura citruspsorosisviruscoatproteinderivedhairpinconstructconfersstabletransgenicresistanceincitrusagainstpsorosisaandbsyndromes |