Immune response of heifers against a Staphylococcus aureus CP5 whole cell vaccine formulated with ISCOMATRIX™ adjuvant
The shortcomings of Staphylococcus aureus vaccines to control bovine mastitis have been attributed to insufficient capacity of the vaccines to induce opsonizing antibodies and to stimulate cellular immune responses. Types of antigen, administration route and adjuvant used in a vaccine formulation ha...
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| Format: | Artículo |
| Language: | Inglés |
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2018
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| Online Access: | https://www.cambridge.org/core/journals/journal-of-dairy-research/article/immune-response-of-heifers-against-a-staphylococcus-aureus-cp5-whole-cell-vaccine-formulated-with-iscomatrix-adjuvant/67FAEF7A88B5AFF6E774F5DEA179FE68 http://hdl.handle.net/20.500.12123/2837 https://doi.org/10.1017/S0022029912000593 |
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| author | Camussone, Cecilia Veaute, Carolina Melania Isabel Porporatto, Carina Morein, Bror Marcipar, Iván Sergio Calvinho, Luis Fernando |
| author_browse | Calvinho, Luis Fernando Camussone, Cecilia Marcipar, Iván Sergio Morein, Bror Porporatto, Carina Veaute, Carolina Melania Isabel |
| author_facet | Camussone, Cecilia Veaute, Carolina Melania Isabel Porporatto, Carina Morein, Bror Marcipar, Iván Sergio Calvinho, Luis Fernando |
| author_sort | Camussone, Cecilia |
| collection | INTA Digital |
| description | The shortcomings of Staphylococcus aureus vaccines to control bovine mastitis have been attributed to insufficient capacity of the vaccines to induce opsonizing antibodies and to stimulate cellular immune responses. Types of antigen, administration route and adjuvant used in a vaccine formulation have been identified as critical factors for the development of opsonic antibodies. Current commercially available vaccines for Staph. aureus bovine mastitis control are formulated with Al(OH)3 and oil-based adjuvants. The aim of this study was to evaluate the immune response of heifers immunized with a Staph. aureus CP5 whole cell vaccine formulated either with Al(OH)3 or ISCOMATRIX™. Twenty primigravid Holstein dairy heifers in the last trimester of gestation were immunized either with a vaccine formulated with ISCOMATRIX™ (n = 6), Al(OH)3 (n = 7), or saline solution (placebo) (n = 7). Immunization was carried out 38 and 10 d before calving. Heifers vaccinated with Staph. aureus adjuvanted with ISCOMATRIX™ responded with significantly higher levels of anti-bacterin and anti-CP5 IgG and IgG2 in sera than animals in the Al(OH)3 or control groups. Animals in the ISCOMATRIX™ group responded with significantly higher anti-bacterin specific IgG in whey than animals in the Al(OH)3 and control groups, detected from the first week post calving until 60 d of lactation. Sera from animals inoculated with Staph. aureus in ISCOMATRIX™, obtained 7 d post partum, significantly increased both the number of neutrophils ingesting bacteria and the number of bacteria being ingested by the neutrophils, compared with sera obtained from heifers vaccinated with Al(OH)3 or non-vaccinated controls. These features coupled to safety of the ISCOMATRIX™ formulation, warrant additional studies. |
| format | Artículo |
| id | INTA2837 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA28372018-07-20T13:26:12Z Immune response of heifers against a Staphylococcus aureus CP5 whole cell vaccine formulated with ISCOMATRIX™ adjuvant Camussone, Cecilia Veaute, Carolina Melania Isabel Porporatto, Carina Morein, Bror Marcipar, Iván Sergio Calvinho, Luis Fernando Novilla Ganado Bovino Enfermedades de los Animales Staphylococcus aureus Respuesta Inmunológica Coadyuvantes Heifers Cattle Animal Diseases Immune Response Adjuvants ISCOM Matrix The shortcomings of Staphylococcus aureus vaccines to control bovine mastitis have been attributed to insufficient capacity of the vaccines to induce opsonizing antibodies and to stimulate cellular immune responses. Types of antigen, administration route and adjuvant used in a vaccine formulation have been identified as critical factors for the development of opsonic antibodies. Current commercially available vaccines for Staph. aureus bovine mastitis control are formulated with Al(OH)3 and oil-based adjuvants. The aim of this study was to evaluate the immune response of heifers immunized with a Staph. aureus CP5 whole cell vaccine formulated either with Al(OH)3 or ISCOMATRIX™. Twenty primigravid Holstein dairy heifers in the last trimester of gestation were immunized either with a vaccine formulated with ISCOMATRIX™ (n = 6), Al(OH)3 (n = 7), or saline solution (placebo) (n = 7). Immunization was carried out 38 and 10 d before calving. Heifers vaccinated with Staph. aureus adjuvanted with ISCOMATRIX™ responded with significantly higher levels of anti-bacterin and anti-CP5 IgG and IgG2 in sera than animals in the Al(OH)3 or control groups. Animals in the ISCOMATRIX™ group responded with significantly higher anti-bacterin specific IgG in whey than animals in the Al(OH)3 and control groups, detected from the first week post calving until 60 d of lactation. Sera from animals inoculated with Staph. aureus in ISCOMATRIX™, obtained 7 d post partum, significantly increased both the number of neutrophils ingesting bacteria and the number of bacteria being ingested by the neutrophils, compared with sera obtained from heifers vaccinated with Al(OH)3 or non-vaccinated controls. These features coupled to safety of the ISCOMATRIX™ formulation, warrant additional studies. EEA Rafaela Fil: Camussone, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Veaute, Carolina Melania Isabel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina Fil: Porporatto, Carina. Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina. Universidad Nacional de Villa María. Instituto de Ciencias Básicas y Aplicadas; Argentina Fil: Morein, Bror. Uppsala University. Department of Clinical Virology; Suecia Fil: Marcipar, Iván Sergio. Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina 2018-07-20T13:23:24Z 2018-07-20T13:23:24Z 2013-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.cambridge.org/core/journals/journal-of-dairy-research/article/immune-response-of-heifers-against-a-staphylococcus-aureus-cp5-whole-cell-vaccine-formulated-with-iscomatrix-adjuvant/67FAEF7A88B5AFF6E774F5DEA179FE68 http://hdl.handle.net/20.500.12123/2837 0022-0299 1469-7629 https://doi.org/10.1017/S0022029912000593 eng info:eu-repo/semantics/restrictedAccess application/pdf Journal of Dairy Research 80 (1) : 72-80 (February 2013) |
| spellingShingle | Novilla Ganado Bovino Enfermedades de los Animales Staphylococcus aureus Respuesta Inmunológica Coadyuvantes Heifers Cattle Animal Diseases Immune Response Adjuvants ISCOM Matrix Camussone, Cecilia Veaute, Carolina Melania Isabel Porporatto, Carina Morein, Bror Marcipar, Iván Sergio Calvinho, Luis Fernando Immune response of heifers against a Staphylococcus aureus CP5 whole cell vaccine formulated with ISCOMATRIX™ adjuvant |
| title | Immune response of heifers against a Staphylococcus aureus CP5 whole cell vaccine formulated with ISCOMATRIX™ adjuvant |
| title_full | Immune response of heifers against a Staphylococcus aureus CP5 whole cell vaccine formulated with ISCOMATRIX™ adjuvant |
| title_fullStr | Immune response of heifers against a Staphylococcus aureus CP5 whole cell vaccine formulated with ISCOMATRIX™ adjuvant |
| title_full_unstemmed | Immune response of heifers against a Staphylococcus aureus CP5 whole cell vaccine formulated with ISCOMATRIX™ adjuvant |
| title_short | Immune response of heifers against a Staphylococcus aureus CP5 whole cell vaccine formulated with ISCOMATRIX™ adjuvant |
| title_sort | immune response of heifers against a staphylococcus aureus cp5 whole cell vaccine formulated with iscomatrix™ adjuvant |
| topic | Novilla Ganado Bovino Enfermedades de los Animales Staphylococcus aureus Respuesta Inmunológica Coadyuvantes Heifers Cattle Animal Diseases Immune Response Adjuvants ISCOM Matrix |
| url | https://www.cambridge.org/core/journals/journal-of-dairy-research/article/immune-response-of-heifers-against-a-staphylococcus-aureus-cp5-whole-cell-vaccine-formulated-with-iscomatrix-adjuvant/67FAEF7A88B5AFF6E774F5DEA179FE68 http://hdl.handle.net/20.500.12123/2837 https://doi.org/10.1017/S0022029912000593 |
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