Identification and characterization of Rht25, a locus on chromosome arm 6AS affecting wheat plant height, heading time, and spike development
Understanding genes regulating wheat plant height is important to optimize harvest index and maximize grain yield. In modern wheat varieties grown under high-input conditions, the gibberellin-insensitive semi-dwarfing alleles Rht-B1b and Rht-D1b have been used extensively to confer lodging toleran...
| Autores principales: | , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/2836 https://link.springer.com/article/10.1007/s00122-018-3130-6#citeas https://doi.org/10.1007/s00122-018-3130-6 |
| _version_ | 1855035035343650816 |
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| author | Youngjun Mo Vanzetti, Leonardo Sebastian Hale, Iago Spagnolo, Emiliano J. Guidobaldi, Fabio Al-Oboudi, Jassim Odle, Natalie Pearce, Stephen Helguera, Marcelo Dubcovsky, Jorge |
| author_browse | Al-Oboudi, Jassim Dubcovsky, Jorge Guidobaldi, Fabio Hale, Iago Helguera, Marcelo Odle, Natalie Pearce, Stephen Spagnolo, Emiliano J. Vanzetti, Leonardo Sebastian Youngjun Mo |
| author_facet | Youngjun Mo Vanzetti, Leonardo Sebastian Hale, Iago Spagnolo, Emiliano J. Guidobaldi, Fabio Al-Oboudi, Jassim Odle, Natalie Pearce, Stephen Helguera, Marcelo Dubcovsky, Jorge |
| author_sort | Youngjun Mo |
| collection | INTA Digital |
| description | Understanding genes regulating wheat plant height is important to optimize harvest index and maximize grain yield.
In modern wheat varieties grown under high-input conditions, the gibberellin-insensitive semi-dwarfing alleles Rht-B1b and
Rht-D1b have been used extensively to confer lodging tolerance and improve harvest index. However, negative pleiotropic
effects of these alleles (e.g., poor seedling emergence and reduced biomass) can cause yield losses in hot and dry environments.
As part of current efforts to diversify the dwarfing alleles used in wheat breeding, we identified a quantitative trait
locus (QHt.ucw-6AS) affecting plant height in the proximal region of chromosome arm 6AS (< 0.4 cM from the centromere).
Using a large segregating population (~ 2800 gametes) and extensive progeny tests (70–93 plants per recombinant family),
we mapped QHt.ucw-6AS as a Mendelian locus to a 0.2 cM interval (144.0–148.3 Mb, IWGSC Ref Seq v1.0) and show that
it is different from Rht18. QHt.ucw-6AS is officially designated as Rht25, with Rht25a representing the height-increasing
allele and Rht25b the dwarfing allele. The average dwarfing effect of Rht25b was found to be approximately half of the effect
observed for Rht-B1b and Rht-D1b, and the effect is greater in the presence of the height-increasing Rht-B1a and Rht-D1a
alleles than in the presence of the dwarfing alleles. Rht25b is gibberellin-sensitive and shows significant pleiotropic effects
on coleoptile length, heading date, spike length, spikelet number, spikelet density, and grain weight. Rht25 represents a new
alternative dwarfing locus that should be evaluated for its potential to improve wheat yield in different environments. |
| format | info:ar-repo/semantics/artículo |
| id | INTA2836 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA28362018-07-20T13:13:05Z Identification and characterization of Rht25, a locus on chromosome arm 6AS affecting wheat plant height, heading time, and spike development Youngjun Mo Vanzetti, Leonardo Sebastian Hale, Iago Spagnolo, Emiliano J. Guidobaldi, Fabio Al-Oboudi, Jassim Odle, Natalie Pearce, Stephen Helguera, Marcelo Dubcovsky, Jorge Trigo Triticum Aestivum Loci Espigas Altura Genes Etapas de Desarrollo de la Planta Plant Developmental Stages Height Spikes Wheat Rht25 Cromosoma 6AS Understanding genes regulating wheat plant height is important to optimize harvest index and maximize grain yield. In modern wheat varieties grown under high-input conditions, the gibberellin-insensitive semi-dwarfing alleles Rht-B1b and Rht-D1b have been used extensively to confer lodging tolerance and improve harvest index. However, negative pleiotropic effects of these alleles (e.g., poor seedling emergence and reduced biomass) can cause yield losses in hot and dry environments. As part of current efforts to diversify the dwarfing alleles used in wheat breeding, we identified a quantitative trait locus (QHt.ucw-6AS) affecting plant height in the proximal region of chromosome arm 6AS (< 0.4 cM from the centromere). Using a large segregating population (~ 2800 gametes) and extensive progeny tests (70–93 plants per recombinant family), we mapped QHt.ucw-6AS as a Mendelian locus to a 0.2 cM interval (144.0–148.3 Mb, IWGSC Ref Seq v1.0) and show that it is different from Rht18. QHt.ucw-6AS is officially designated as Rht25, with Rht25a representing the height-increasing allele and Rht25b the dwarfing allele. The average dwarfing effect of Rht25b was found to be approximately half of the effect observed for Rht-B1b and Rht-D1b, and the effect is greater in the presence of the height-increasing Rht-B1a and Rht-D1a alleles than in the presence of the dwarfing alleles. Rht25b is gibberellin-sensitive and shows significant pleiotropic effects on coleoptile length, heading date, spike length, spikelet number, spikelet density, and grain weight. Rht25 represents a new alternative dwarfing locus that should be evaluated for its potential to improve wheat yield in different environments. EEA Marcos Juárez Fil: Youngjun, Mo. University of California. Department of Plant Sciences; Estados Unidos. Rural Development Administration. National Institute of Crop Science, Wanju; Corea del Sur Fil: Vanzetti, Leonardo Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Hale, Iago. University of New Hampshire. Department of Agriculture, Nutrition, and Food Systems; Estados Unidos Fil: Spagnolo, Emiliano J. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Guidobaldi, Fabio. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; Argentina Fil: Al-Oboudi, Jassim. University of California. Department of Plant Sciences; Estados Unidos Fil: Odle, Natalie. University of California. Department of Plant Sciences; Estados Unidos Fil: Pearce, Stephen. Colorado State University. Department of Soil and Crop Sciences; Estados Unidos Fil: Helguera, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Dubcovsky, Jorge. University of California. Department of Plant Sciences; Estados Unidos. Howard Hughes Medical Institute; Estados Unidos 2018-07-20T13:06:06Z 2018-07-20T13:06:06Z 2018 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/2836 https://link.springer.com/article/10.1007/s00122-018-3130-6#citeas 0040-5752 1432-2242 (Online) https://doi.org/10.1007/s00122-018-3130-6 eng info:eu-repo/semantics/restrictedAccess application/pdf Theoretical and applied genetics 1-15. (2018) |
| spellingShingle | Trigo Triticum Aestivum Loci Espigas Altura Genes Etapas de Desarrollo de la Planta Plant Developmental Stages Height Spikes Wheat Rht25 Cromosoma 6AS Youngjun Mo Vanzetti, Leonardo Sebastian Hale, Iago Spagnolo, Emiliano J. Guidobaldi, Fabio Al-Oboudi, Jassim Odle, Natalie Pearce, Stephen Helguera, Marcelo Dubcovsky, Jorge Identification and characterization of Rht25, a locus on chromosome arm 6AS affecting wheat plant height, heading time, and spike development |
| title | Identification and characterization of Rht25, a locus on chromosome arm 6AS affecting wheat plant height, heading time, and spike development |
| title_full | Identification and characterization of Rht25, a locus on chromosome arm 6AS affecting wheat plant height, heading time, and spike development |
| title_fullStr | Identification and characterization of Rht25, a locus on chromosome arm 6AS affecting wheat plant height, heading time, and spike development |
| title_full_unstemmed | Identification and characterization of Rht25, a locus on chromosome arm 6AS affecting wheat plant height, heading time, and spike development |
| title_short | Identification and characterization of Rht25, a locus on chromosome arm 6AS affecting wheat plant height, heading time, and spike development |
| title_sort | identification and characterization of rht25 a locus on chromosome arm 6as affecting wheat plant height heading time and spike development |
| topic | Trigo Triticum Aestivum Loci Espigas Altura Genes Etapas de Desarrollo de la Planta Plant Developmental Stages Height Spikes Wheat Rht25 Cromosoma 6AS |
| url | http://hdl.handle.net/20.500.12123/2836 https://link.springer.com/article/10.1007/s00122-018-3130-6#citeas https://doi.org/10.1007/s00122-018-3130-6 |
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