Staphylococcus aureus chronic intramammary infection modifies protein expression of transforming growth factor beta (TGF-β) subfamily components during active involution
The objectives of this study were to determine whether Staphylococcus aureus chronic intramammary infection (IMI) influences protein expression of TGF-β subfamily components and collagen I and to examine the histomorphometric changes that occur in mammary stroma and parenchyma during active mammary...
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| Format: | Artículo |
| Language: | Inglés |
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2018
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| Online Access: | https://www.sciencedirect.com/science/article/pii/S0034528813003330 http://hdl.handle.net/20.500.12123/2835 https://doi.org/10.1016/j.rvsc.2013.11.002 |
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| author | Andreotti, Carolina Soledad Pereyra, Elizabet Amanda Lorena Baravalle, Celina Renna, Maria Sol Ortega, Hugo Hector Calvinho, Luis Fernando Dallard, Bibiana Elisabet |
| author_browse | Andreotti, Carolina Soledad Baravalle, Celina Calvinho, Luis Fernando Dallard, Bibiana Elisabet Ortega, Hugo Hector Pereyra, Elizabet Amanda Lorena Renna, Maria Sol |
| author_facet | Andreotti, Carolina Soledad Pereyra, Elizabet Amanda Lorena Baravalle, Celina Renna, Maria Sol Ortega, Hugo Hector Calvinho, Luis Fernando Dallard, Bibiana Elisabet |
| author_sort | Andreotti, Carolina Soledad |
| collection | INTA Digital |
| description | The objectives of this study were to determine whether Staphylococcus aureus chronic intramammary infection (IMI) influences protein expression of TGF-β subfamily components and collagen I and to examine the histomorphometric changes that occur in mammary stroma and parenchyma during active mammary gland involution. Twenty-one Holstein non-pregnant cows in late lactation either uninfected or with chronic natural S. aureus IMI were included in this study. Cows were slaughtered at 7, 14 and 21 d after cessation of milking and samples for immunohistochemical and morphometric analysis were taken. Protein expression of TGF-β1, TGF-β2 and TGF-β3 was significantly higher in chronically infected quarters than in uninfected controls at the three involution stages studied. Immunostaining of TGF-βR1 and TGF-βR3 and collagen I was significantly higher in S. aureus-infected quarters than in uninfected controls at every involution time evaluated. The percentages of tissue area composed of parenchyma and intralobular stroma were significantly higher in S. aureus-infected than in uninfected quarters. Chronic S. aureus mastitis modifies protein expression of the three TGF-β isoforms and type 1 and 3 receptors, which was associated with changes directed to limit the scope of inflammation and injury to the host. |
| format | Artículo |
| id | INTA2835 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA28352018-07-20T13:04:37Z Staphylococcus aureus chronic intramammary infection modifies protein expression of transforming growth factor beta (TGF-β) subfamily components during active involution Andreotti, Carolina Soledad Pereyra, Elizabet Amanda Lorena Baravalle, Celina Renna, Maria Sol Ortega, Hugo Hector Calvinho, Luis Fernando Dallard, Bibiana Elisabet Staphylococcus aureus Enfermedades de los Animales Mastítis Bovina Ganado Bovino Animal Diseases Bovine Mastitis Cattle Infección Intramamaria Crónica The objectives of this study were to determine whether Staphylococcus aureus chronic intramammary infection (IMI) influences protein expression of TGF-β subfamily components and collagen I and to examine the histomorphometric changes that occur in mammary stroma and parenchyma during active mammary gland involution. Twenty-one Holstein non-pregnant cows in late lactation either uninfected or with chronic natural S. aureus IMI were included in this study. Cows were slaughtered at 7, 14 and 21 d after cessation of milking and samples for immunohistochemical and morphometric analysis were taken. Protein expression of TGF-β1, TGF-β2 and TGF-β3 was significantly higher in chronically infected quarters than in uninfected controls at the three involution stages studied. Immunostaining of TGF-βR1 and TGF-βR3 and collagen I was significantly higher in S. aureus-infected quarters than in uninfected controls at every involution time evaluated. The percentages of tissue area composed of parenchyma and intralobular stroma were significantly higher in S. aureus-infected than in uninfected quarters. Chronic S. aureus mastitis modifies protein expression of the three TGF-β isoforms and type 1 and 3 receptors, which was associated with changes directed to limit the scope of inflammation and injury to the host. EEA Rafaela Fil: Andreotti, Carolina Soledad. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina. Fil: Pereyra, Elizabet Amanda Lorena. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina. Fil: Baravalle, Celina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Renna, María Sol. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Ortega, Hugo Hector. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina. Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina Fil: Dallard, Bibiana Elisabet. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina. 2018-07-20T13:03:19Z 2018-07-20T13:03:19Z 2014-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0034528813003330 http://hdl.handle.net/20.500.12123/2835 0034-5288 1532-2661 https://doi.org/10.1016/j.rvsc.2013.11.002 eng info:eu-repo/semantics/restrictedAccess application/pdf Research in Veterinary Science 96 (1) : 5-14 (February 2014) |
| spellingShingle | Staphylococcus aureus Enfermedades de los Animales Mastítis Bovina Ganado Bovino Animal Diseases Bovine Mastitis Cattle Infección Intramamaria Crónica Andreotti, Carolina Soledad Pereyra, Elizabet Amanda Lorena Baravalle, Celina Renna, Maria Sol Ortega, Hugo Hector Calvinho, Luis Fernando Dallard, Bibiana Elisabet Staphylococcus aureus chronic intramammary infection modifies protein expression of transforming growth factor beta (TGF-β) subfamily components during active involution |
| title | Staphylococcus aureus chronic intramammary infection modifies protein expression of transforming growth factor beta (TGF-β) subfamily components during active involution |
| title_full | Staphylococcus aureus chronic intramammary infection modifies protein expression of transforming growth factor beta (TGF-β) subfamily components during active involution |
| title_fullStr | Staphylococcus aureus chronic intramammary infection modifies protein expression of transforming growth factor beta (TGF-β) subfamily components during active involution |
| title_full_unstemmed | Staphylococcus aureus chronic intramammary infection modifies protein expression of transforming growth factor beta (TGF-β) subfamily components during active involution |
| title_short | Staphylococcus aureus chronic intramammary infection modifies protein expression of transforming growth factor beta (TGF-β) subfamily components during active involution |
| title_sort | staphylococcus aureus chronic intramammary infection modifies protein expression of transforming growth factor beta tgf β subfamily components during active involution |
| topic | Staphylococcus aureus Enfermedades de los Animales Mastítis Bovina Ganado Bovino Animal Diseases Bovine Mastitis Cattle Infección Intramamaria Crónica |
| url | https://www.sciencedirect.com/science/article/pii/S0034528813003330 http://hdl.handle.net/20.500.12123/2835 https://doi.org/10.1016/j.rvsc.2013.11.002 |
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