Immune response of heifers against a Staphylococcus aureus CP5 whole cell and lysate vaccine formulated with ISCOM Matrix adjuvant
Staphylococcus aureus is the most frequently isolated pathogen from bovine intramammary infections worldwide. Commercially available vaccines for mastitis control are composed either of S. aureus lysates or whole-cells formulated with traditional adjuvants. We recently showed the ability of a S. aur...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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2018
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| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S0034528813003214 http://hdl.handle.net/20.500.12123/2834 https://doi.org/10.1016/j.rvsc.2013.10.004 |
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| author | Camussone, Cecilia Veaute, Carolina Melania Isabel Pujato, Nazarena Morein, Bror Marcipar, Iván Sergio Calvinho, Luis Fernando |
| author_browse | Calvinho, Luis Fernando Camussone, Cecilia Marcipar, Iván Sergio Morein, Bror Pujato, Nazarena Veaute, Carolina Melania Isabel |
| author_facet | Camussone, Cecilia Veaute, Carolina Melania Isabel Pujato, Nazarena Morein, Bror Marcipar, Iván Sergio Calvinho, Luis Fernando |
| author_sort | Camussone, Cecilia |
| collection | INTA Digital |
| description | Staphylococcus aureus is the most frequently isolated pathogen from bovine intramammary infections worldwide. Commercially available vaccines for mastitis control are composed either of S. aureus lysates or whole-cells formulated with traditional adjuvants. We recently showed the ability of a S. aureus CP5 whole-cell vaccine adjuvanted with ISCOM Matrix to increase specific antibodies production in blood and milk, improving opsonic capacity, compared with the same vaccine formulated with Al(OH)3. However, there is no information about the use of ISCOM Matrix for the formulation of bacterial lysates. The aim of this study was to characterize the innate and humoral immune responses induced by a S. aureus CP5 whole-cell or lysate vaccine, formulated with ISCOM Matrix after immunization of pregnant heifers. Both immunogens stimulated strong humoral immune responses in blood and milk, raising antibodies that increased opsonic capacity. Lysate formulation generated a higher and longer lasting antibody titer and stimulated a higher expression of regulatory and pro-inflammatory cytokines compared with the whole-cell vaccine. |
| format | Artículo |
| id | INTA2834 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA28342018-07-20T12:15:02Z Immune response of heifers against a Staphylococcus aureus CP5 whole cell and lysate vaccine formulated with ISCOM Matrix adjuvant Camussone, Cecilia Veaute, Carolina Melania Isabel Pujato, Nazarena Morein, Bror Marcipar, Iván Sergio Calvinho, Luis Fernando Novilla Ganado Bovino Enfermedades de los Animales Staphylococcus aureus Vacuna Respuesta Inmunológica Coadyuvantes Heifers Cattle Animal Diseases Vaccines Immune Response Adjuvants ISCOM Matrix Staphylococcus aureus is the most frequently isolated pathogen from bovine intramammary infections worldwide. Commercially available vaccines for mastitis control are composed either of S. aureus lysates or whole-cells formulated with traditional adjuvants. We recently showed the ability of a S. aureus CP5 whole-cell vaccine adjuvanted with ISCOM Matrix to increase specific antibodies production in blood and milk, improving opsonic capacity, compared with the same vaccine formulated with Al(OH)3. However, there is no information about the use of ISCOM Matrix for the formulation of bacterial lysates. The aim of this study was to characterize the innate and humoral immune responses induced by a S. aureus CP5 whole-cell or lysate vaccine, formulated with ISCOM Matrix after immunization of pregnant heifers. Both immunogens stimulated strong humoral immune responses in blood and milk, raising antibodies that increased opsonic capacity. Lysate formulation generated a higher and longer lasting antibody titer and stimulated a higher expression of regulatory and pro-inflammatory cytokines compared with the whole-cell vaccine. EEA Rafaela Fil: Camussone, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Veaute, Carolina Melania Isabel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina Fil: Pujato, Nazarena. Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina Fil: Morein, Bror. Uppsala University. Department of Clinical Virology; Suecia Fil: Marcipar, Iván Sergio. Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina 2018-07-20T12:09:39Z 2018-07-20T12:09:39Z 2014-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0034528813003214 http://hdl.handle.net/20.500.12123/2834 0034-5288 1532-2661 https://doi.org/10.1016/j.rvsc.2013.10.004 eng info:eu-repo/semantics/restrictedAccess application/pdf Research in Veterinary Science 96 (1) : 86-94 (February 2014) |
| spellingShingle | Novilla Ganado Bovino Enfermedades de los Animales Staphylococcus aureus Vacuna Respuesta Inmunológica Coadyuvantes Heifers Cattle Animal Diseases Vaccines Immune Response Adjuvants ISCOM Matrix Camussone, Cecilia Veaute, Carolina Melania Isabel Pujato, Nazarena Morein, Bror Marcipar, Iván Sergio Calvinho, Luis Fernando Immune response of heifers against a Staphylococcus aureus CP5 whole cell and lysate vaccine formulated with ISCOM Matrix adjuvant |
| title | Immune response of heifers against a Staphylococcus aureus CP5 whole cell and lysate vaccine formulated with ISCOM Matrix adjuvant |
| title_full | Immune response of heifers against a Staphylococcus aureus CP5 whole cell and lysate vaccine formulated with ISCOM Matrix adjuvant |
| title_fullStr | Immune response of heifers against a Staphylococcus aureus CP5 whole cell and lysate vaccine formulated with ISCOM Matrix adjuvant |
| title_full_unstemmed | Immune response of heifers against a Staphylococcus aureus CP5 whole cell and lysate vaccine formulated with ISCOM Matrix adjuvant |
| title_short | Immune response of heifers against a Staphylococcus aureus CP5 whole cell and lysate vaccine formulated with ISCOM Matrix adjuvant |
| title_sort | immune response of heifers against a staphylococcus aureus cp5 whole cell and lysate vaccine formulated with iscom matrix adjuvant |
| topic | Novilla Ganado Bovino Enfermedades de los Animales Staphylococcus aureus Vacuna Respuesta Inmunológica Coadyuvantes Heifers Cattle Animal Diseases Vaccines Immune Response Adjuvants ISCOM Matrix |
| url | https://www.sciencedirect.com/science/article/pii/S0034528813003214 http://hdl.handle.net/20.500.12123/2834 https://doi.org/10.1016/j.rvsc.2013.10.004 |
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