Characterization of sensitivity of grove and packing house isolates of Penicillium digitatum to pyrimethanil
In most northeast Argentinean citrus packing houses, postharvest fungicide treatments are based onthe use of thiabendazole and imazalil. However, these fungicides have been used in a manner highlyconducive to the selection and proliferation of resistant biotypes of Penicillium digitatum, the main fr...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/2832 https://doi.org/10.1016/j.postharvbio.2014.06.012 |
| _version_ | 1855483128605310976 |
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| author | Vazquez, Daniel Eduardo Panozzo, Marina Graciela Almirón, Nanci Jacqueline Bello, Fernando Burdyn, Lourdes Garran, Sergio Mario |
| author_browse | Almirón, Nanci Jacqueline Bello, Fernando Burdyn, Lourdes Garran, Sergio Mario Panozzo, Marina Graciela Vazquez, Daniel Eduardo |
| author_facet | Vazquez, Daniel Eduardo Panozzo, Marina Graciela Almirón, Nanci Jacqueline Bello, Fernando Burdyn, Lourdes Garran, Sergio Mario |
| author_sort | Vazquez, Daniel Eduardo |
| collection | INTA Digital |
| description | In most northeast Argentinean citrus packing houses, postharvest fungicide treatments are based onthe use of thiabendazole and imazalil. However, these fungicides have been used in a manner highlyconducive to the selection and proliferation of resistant biotypes of Penicillium digitatum, the main fruitdecay fungus in the area. Recently, a new fungicide, pyrimethanil (PYR), was introduced to control molds.Aims of this study were to determine the baseline sensitivities for PYR against isolates of P. digitatumconsidering its use in the region is not yet widespread and to evaluate the control of the fungus in vivo.One hundred and nine (109) P. digitatum isolates were collected from diseased fruit within citrus groves(43 isolates) and packing houses (66 isolates). EC50was determined for each isolate by measuring colonydiameters on different agar dilutions of the fungicide. The mean EC50value of the green mold isolatescollected from the groves was 0.14 ± 0.03 mg L−1while the mean EC50of those collected from packinghouses was 0.13 ± 0.05 mg L−1. No resistant isolates were found in the field where the fungicide is notused, while one isolate originated from a packing house showed an EC50of 3.40 mg L−1, 26-fold higherthan the mean level. This isolate was collected from lemons stored in cool rooms of a packing house wherePYR had not been used. Fruit decay by sensitive isolates was reduced approximately 80% by PYR appliedat 500–600 mg L−1by immersion for 60 s at room temperature to inoculated oranges and mandarins. Incontrast, the resistant isolate was not controlled by PYR applied at 1000 mg L−1. Thus, the introduction ofPYR applied into packing houses should be done carefully and control strategies should be implementedin order to minimize the development of resistant isolates. |
| format | Artículo |
| id | INTA2832 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA28322018-08-07T18:08:55Z Characterization of sensitivity of grove and packing house isolates of Penicillium digitatum to pyrimethanil Vazquez, Daniel Eduardo Panozzo, Marina Graciela Almirón, Nanci Jacqueline Bello, Fernando Burdyn, Lourdes Garran, Sergio Mario Resistencia a los Fungicidas Penicillium Digitatum Citrus Contaminación Biológica Tecnología Postcosecha Postharvest Technology Packaging Resistance to Fungicides Empaquetado Pyrimethanil Baseline Sensitivity In most northeast Argentinean citrus packing houses, postharvest fungicide treatments are based onthe use of thiabendazole and imazalil. However, these fungicides have been used in a manner highlyconducive to the selection and proliferation of resistant biotypes of Penicillium digitatum, the main fruitdecay fungus in the area. Recently, a new fungicide, pyrimethanil (PYR), was introduced to control molds.Aims of this study were to determine the baseline sensitivities for PYR against isolates of P. digitatumconsidering its use in the region is not yet widespread and to evaluate the control of the fungus in vivo.One hundred and nine (109) P. digitatum isolates were collected from diseased fruit within citrus groves(43 isolates) and packing houses (66 isolates). EC50was determined for each isolate by measuring colonydiameters on different agar dilutions of the fungicide. The mean EC50value of the green mold isolatescollected from the groves was 0.14 ± 0.03 mg L−1while the mean EC50of those collected from packinghouses was 0.13 ± 0.05 mg L−1. No resistant isolates were found in the field where the fungicide is notused, while one isolate originated from a packing house showed an EC50of 3.40 mg L−1, 26-fold higherthan the mean level. This isolate was collected from lemons stored in cool rooms of a packing house wherePYR had not been used. Fruit decay by sensitive isolates was reduced approximately 80% by PYR appliedat 500–600 mg L−1by immersion for 60 s at room temperature to inoculated oranges and mandarins. Incontrast, the resistant isolate was not controlled by PYR applied at 1000 mg L−1. Thus, the introduction ofPYR applied into packing houses should be done carefully and control strategies should be implementedin order to minimize the development of resistant isolates. EEA Concordia Fil: Vazquez, Daniel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Panozzo, Marina Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Almiron, Nanci Jacqueline. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Bello, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Burdyn, Lourdes. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Garran, Sergio Mario. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina 2018-07-19T18:10:14Z 2018-07-19T18:10:14Z 2014 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/2832 0925-5214 https://doi.org/10.1016/j.postharvbio.2014.06.012 eng info:eu-repo/semantics/restrictedAccess application/pdf Postharvest biology and technology 98 : 1–6. (2014) |
| spellingShingle | Resistencia a los Fungicidas Penicillium Digitatum Citrus Contaminación Biológica Tecnología Postcosecha Postharvest Technology Packaging Resistance to Fungicides Empaquetado Pyrimethanil Baseline Sensitivity Vazquez, Daniel Eduardo Panozzo, Marina Graciela Almirón, Nanci Jacqueline Bello, Fernando Burdyn, Lourdes Garran, Sergio Mario Characterization of sensitivity of grove and packing house isolates of Penicillium digitatum to pyrimethanil |
| title | Characterization of sensitivity of grove and packing house isolates of Penicillium digitatum to pyrimethanil |
| title_full | Characterization of sensitivity of grove and packing house isolates of Penicillium digitatum to pyrimethanil |
| title_fullStr | Characterization of sensitivity of grove and packing house isolates of Penicillium digitatum to pyrimethanil |
| title_full_unstemmed | Characterization of sensitivity of grove and packing house isolates of Penicillium digitatum to pyrimethanil |
| title_short | Characterization of sensitivity of grove and packing house isolates of Penicillium digitatum to pyrimethanil |
| title_sort | characterization of sensitivity of grove and packing house isolates of penicillium digitatum to pyrimethanil |
| topic | Resistencia a los Fungicidas Penicillium Digitatum Citrus Contaminación Biológica Tecnología Postcosecha Postharvest Technology Packaging Resistance to Fungicides Empaquetado Pyrimethanil Baseline Sensitivity |
| url | http://hdl.handle.net/20.500.12123/2832 https://doi.org/10.1016/j.postharvbio.2014.06.012 |
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