Functional role of antibodies generated in heifers through immunization with Staphylococcus aureus vaccines in invasion and phagocytosis assays
A successful Staphylococcus aureus vaccine should elicit a long-term antibody response that prevents establishment of the infection. The aim of the present study was to evaluate the functional role of antibodies raised against different S. aureus CP5 vaccines in invasion to bovine mammary epithelial...
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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2018
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| Acceso en línea: | https://academic.oup.com/femsle/article/360/1/62/2683998 http://hdl.handle.net/20.500.12123/2828 https://doi.org/10.1111/1574-6968.12588 |
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| author | Renna, María Sol Pereyra, Elizabet Amanda Lorena Baravalle, Celina Camussone, Cecilia Dallard, Bibiana Elisabet Marcipar, Iván Sergio Calvinho, Luis Fernando |
| author_browse | Baravalle, Celina Calvinho, Luis Fernando Camussone, Cecilia Dallard, Bibiana Elisabet Marcipar, Iván Sergio Pereyra, Elizabet Amanda Lorena Renna, María Sol |
| author_facet | Renna, María Sol Pereyra, Elizabet Amanda Lorena Baravalle, Celina Camussone, Cecilia Dallard, Bibiana Elisabet Marcipar, Iván Sergio Calvinho, Luis Fernando |
| author_sort | Renna, María Sol |
| collection | INTA Digital |
| description | A successful Staphylococcus aureus vaccine should elicit a long-term antibody response that prevents establishment of the infection. The aim of the present study was to evaluate the functional role of antibodies raised against different S. aureus CP5 vaccines in invasion to bovine mammary epithelial cells (MAC-T) and phagocytosis by bovine milk macrophages in vitro. Sera and whey from cows immunized with a whole-cell S. aureus CP5 vaccine adjuvanted with Al(OH)3 or with ISCOM Matrix, significantly reduced internalization of S. aureus in MAC-T cells without significant differences between both groups. The effect of antibodies generated by a S. aureus whole-cell and a lysate vaccine formulated with ISCOM Matrix was also evaluated. Sera and whey from both immunized groups significantly reduced S. aureus internalization in MAC-T cells without significant differences between both groups. Whey antibodies against whole-cell and lysate vaccines were also able to inhibit internalization in MAC-T cells of a heterologous S. aureus strain. In addition, sera from animals vaccinated with S. aureus lysate or bacterin promoted milk macrophage phagocytosis. These results provide an insight into the potential mechanisms by which these vaccines can afford protection to the mammary gland against S. aureus intramammary infection. |
| format | info:ar-repo/semantics/artículo |
| id | INTA2828 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA28282019-09-11T18:36:17Z Functional role of antibodies generated in heifers through immunization with Staphylococcus aureus vaccines in invasion and phagocytosis assays Renna, María Sol Pereyra, Elizabet Amanda Lorena Baravalle, Celina Camussone, Cecilia Dallard, Bibiana Elisabet Marcipar, Iván Sergio Calvinho, Luis Fernando Ganado Bovino Novilla Enfermedades de los Animales Control de Enfermedades Mastítis Bovina Staphylococcus aureus Vacuna Anticuerpos Cattle Heifers Animal Diseases Disease Control Bovine Mastitis Vaccines Antibodies Phagocytosis Fagocitosis A successful Staphylococcus aureus vaccine should elicit a long-term antibody response that prevents establishment of the infection. The aim of the present study was to evaluate the functional role of antibodies raised against different S. aureus CP5 vaccines in invasion to bovine mammary epithelial cells (MAC-T) and phagocytosis by bovine milk macrophages in vitro. Sera and whey from cows immunized with a whole-cell S. aureus CP5 vaccine adjuvanted with Al(OH)3 or with ISCOM Matrix, significantly reduced internalization of S. aureus in MAC-T cells without significant differences between both groups. The effect of antibodies generated by a S. aureus whole-cell and a lysate vaccine formulated with ISCOM Matrix was also evaluated. Sera and whey from both immunized groups significantly reduced S. aureus internalization in MAC-T cells without significant differences between both groups. Whey antibodies against whole-cell and lysate vaccines were also able to inhibit internalization in MAC-T cells of a heterologous S. aureus strain. In addition, sera from animals vaccinated with S. aureus lysate or bacterin promoted milk macrophage phagocytosis. These results provide an insight into the potential mechanisms by which these vaccines can afford protection to the mammary gland against S. aureus intramammary infection. EEA Rafaela Fil: Renna, Maria Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina Fil: Pereyra, Elizabet Amanda Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Biología Celular y Molecular Aplicada; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina Fil: Baravalle, Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Biología Celular y Molecular Aplicada; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina Fil: Camussone, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Dallard, Bibiana Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Biología Celular y Molecular Aplicada; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina Fil: Marcipar, Iván Sergio. Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina 2018-07-19T14:48:39Z 2018-07-19T14:48:39Z 2014-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://academic.oup.com/femsle/article/360/1/62/2683998 http://hdl.handle.net/20.500.12123/2828 0378-1097 1574-6968 https://doi.org/10.1111/1574-6968.12588 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf FEMS Microbiology Letters 360 (1) : 62–69 (November 2014) |
| spellingShingle | Ganado Bovino Novilla Enfermedades de los Animales Control de Enfermedades Mastítis Bovina Staphylococcus aureus Vacuna Anticuerpos Cattle Heifers Animal Diseases Disease Control Bovine Mastitis Vaccines Antibodies Phagocytosis Fagocitosis Renna, María Sol Pereyra, Elizabet Amanda Lorena Baravalle, Celina Camussone, Cecilia Dallard, Bibiana Elisabet Marcipar, Iván Sergio Calvinho, Luis Fernando Functional role of antibodies generated in heifers through immunization with Staphylococcus aureus vaccines in invasion and phagocytosis assays |
| title | Functional role of antibodies generated in heifers through immunization with Staphylococcus aureus vaccines in invasion and phagocytosis assays |
| title_full | Functional role of antibodies generated in heifers through immunization with Staphylococcus aureus vaccines in invasion and phagocytosis assays |
| title_fullStr | Functional role of antibodies generated in heifers through immunization with Staphylococcus aureus vaccines in invasion and phagocytosis assays |
| title_full_unstemmed | Functional role of antibodies generated in heifers through immunization with Staphylococcus aureus vaccines in invasion and phagocytosis assays |
| title_short | Functional role of antibodies generated in heifers through immunization with Staphylococcus aureus vaccines in invasion and phagocytosis assays |
| title_sort | functional role of antibodies generated in heifers through immunization with staphylococcus aureus vaccines in invasion and phagocytosis assays |
| topic | Ganado Bovino Novilla Enfermedades de los Animales Control de Enfermedades Mastítis Bovina Staphylococcus aureus Vacuna Anticuerpos Cattle Heifers Animal Diseases Disease Control Bovine Mastitis Vaccines Antibodies Phagocytosis Fagocitosis |
| url | https://academic.oup.com/femsle/article/360/1/62/2683998 http://hdl.handle.net/20.500.12123/2828 https://doi.org/10.1111/1574-6968.12588 |
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