Immune response and functional role of antibodies raised in heifers against a Staphylococcus aureus CP5 lysate and recombinant antigens vaccine formulated with Iscom Matrix adjuvant
Staphylococcus aureus is the most frequently isolated pathogen from bovine intramammary infections worldwide. Commercially available vaccines for mastitis control are composed either of S. aureus lysates or inactivated whole-cells formulated with traditional adjuvants. We recently showed the ability...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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2018
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| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S0165242714002293 http://hdl.handle.net/20.500.12123/2826 https://doi.org/10.1016/j.vetimm.2014.10.004 |
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| author | Camussone, Cecilia Pujato, Nazarena Renna, María Sol Veaute, Carolina Melania Isabel Morein, Bror Marcipar, Iván Sergio Calvinho, Luis Fernando |
| author_browse | Calvinho, Luis Fernando Camussone, Cecilia Marcipar, Iván Sergio Morein, Bror Pujato, Nazarena Renna, María Sol Veaute, Carolina Melania Isabel |
| author_facet | Camussone, Cecilia Pujato, Nazarena Renna, María Sol Veaute, Carolina Melania Isabel Morein, Bror Marcipar, Iván Sergio Calvinho, Luis Fernando |
| author_sort | Camussone, Cecilia |
| collection | INTA Digital |
| description | Staphylococcus aureus is the most frequently isolated pathogen from bovine intramammary infections worldwide. Commercially available vaccines for mastitis control are composed either of S. aureus lysates or inactivated whole-cells formulated with traditional adjuvants. We recently showed the ability of a S. aureus CP5 lysate vaccine adjuvanted with Iscom Matrix to generate a longer lasting specific antibody response in blood and milk, with improved opsonic capacity, compared with a S. aureus CP5 whole-cell formulation. The aim of the present study was to obtain an experimental immunogen composed of lysed cells of a CP5 S. aureus strain supplemented with recombinant clumping factor A, fibronectin binding protein A and β-toxin formulated with Iscom Matrix, characterize the immune response generated when immunizing pregnant heifers and assess the functional role of antibodies raised against this immunogen in experimental models. Both a lysate vaccine and a lysate + recombinant antigens vaccine elicited antibodies that promoted neutrophil phagocytosis and inhibited internalization into mammary epithelial cells, in vitro. Incorporation of defined antigenic molecules to the lysate formulation elicited a strong specific humoral immune response against both lysate and recombinant antigens and was associated with higher expression of regulatory and pro-inflammatory cytokines. In addition, antibodies were efficient for blocking S. aureus binding to bovine fibrinogen and fibronectin, and neutralizing β-toxin effect in vitro, placing these antigens as candidates to be included in a formulation directed to prevent staphylococcal bovine mastitis. |
| format | Artículo |
| id | INTA2826 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA28262018-07-20T12:08:05Z Immune response and functional role of antibodies raised in heifers against a Staphylococcus aureus CP5 lysate and recombinant antigens vaccine formulated with Iscom Matrix adjuvant Camussone, Cecilia Pujato, Nazarena Renna, María Sol Veaute, Carolina Melania Isabel Morein, Bror Marcipar, Iván Sergio Calvinho, Luis Fernando Ganado Bovino Mastítis Bovina Enfermedades de los Animales Staphylococcus aureus Anticuerpos Novilla Respuesta Inmunológica Vacuna Cattle Bovine Mastitis Animal Diseases Antibodies Heifers Immune Response Vaccines ISCOM Matrix Staphylococcus aureus is the most frequently isolated pathogen from bovine intramammary infections worldwide. Commercially available vaccines for mastitis control are composed either of S. aureus lysates or inactivated whole-cells formulated with traditional adjuvants. We recently showed the ability of a S. aureus CP5 lysate vaccine adjuvanted with Iscom Matrix to generate a longer lasting specific antibody response in blood and milk, with improved opsonic capacity, compared with a S. aureus CP5 whole-cell formulation. The aim of the present study was to obtain an experimental immunogen composed of lysed cells of a CP5 S. aureus strain supplemented with recombinant clumping factor A, fibronectin binding protein A and β-toxin formulated with Iscom Matrix, characterize the immune response generated when immunizing pregnant heifers and assess the functional role of antibodies raised against this immunogen in experimental models. Both a lysate vaccine and a lysate + recombinant antigens vaccine elicited antibodies that promoted neutrophil phagocytosis and inhibited internalization into mammary epithelial cells, in vitro. Incorporation of defined antigenic molecules to the lysate formulation elicited a strong specific humoral immune response against both lysate and recombinant antigens and was associated with higher expression of regulatory and pro-inflammatory cytokines. In addition, antibodies were efficient for blocking S. aureus binding to bovine fibrinogen and fibronectin, and neutralizing β-toxin effect in vitro, placing these antigens as candidates to be included in a formulation directed to prevent staphylococcal bovine mastitis. EEA Rafaela Fil: Camussone, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pujato, Nazarena. Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina Fil: Renna, Maria Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina Fil: Veaute, Carolina Melania Isabel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina Fil: Morein, Bror. Uppsala University. Department of Clinical Virology; Suecia Fil: Marcipar, Iván Sergio. Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina 2018-07-19T14:19:09Z 2018-07-19T14:19:09Z 2014-12-15 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0165242714002293 http://hdl.handle.net/20.500.12123/2826 0165-2427 https://doi.org/10.1016/j.vetimm.2014.10.004 eng info:eu-repo/semantics/restrictedAccess application/pdf Veterinary Immunology and Immunopathology 162 (3–4) : 96-107 (December 2014) |
| spellingShingle | Ganado Bovino Mastítis Bovina Enfermedades de los Animales Staphylococcus aureus Anticuerpos Novilla Respuesta Inmunológica Vacuna Cattle Bovine Mastitis Animal Diseases Antibodies Heifers Immune Response Vaccines ISCOM Matrix Camussone, Cecilia Pujato, Nazarena Renna, María Sol Veaute, Carolina Melania Isabel Morein, Bror Marcipar, Iván Sergio Calvinho, Luis Fernando Immune response and functional role of antibodies raised in heifers against a Staphylococcus aureus CP5 lysate and recombinant antigens vaccine formulated with Iscom Matrix adjuvant |
| title | Immune response and functional role of antibodies raised in heifers against a Staphylococcus aureus CP5 lysate and recombinant antigens vaccine formulated with Iscom Matrix adjuvant |
| title_full | Immune response and functional role of antibodies raised in heifers against a Staphylococcus aureus CP5 lysate and recombinant antigens vaccine formulated with Iscom Matrix adjuvant |
| title_fullStr | Immune response and functional role of antibodies raised in heifers against a Staphylococcus aureus CP5 lysate and recombinant antigens vaccine formulated with Iscom Matrix adjuvant |
| title_full_unstemmed | Immune response and functional role of antibodies raised in heifers against a Staphylococcus aureus CP5 lysate and recombinant antigens vaccine formulated with Iscom Matrix adjuvant |
| title_short | Immune response and functional role of antibodies raised in heifers against a Staphylococcus aureus CP5 lysate and recombinant antigens vaccine formulated with Iscom Matrix adjuvant |
| title_sort | immune response and functional role of antibodies raised in heifers against a staphylococcus aureus cp5 lysate and recombinant antigens vaccine formulated with iscom matrix adjuvant |
| topic | Ganado Bovino Mastítis Bovina Enfermedades de los Animales Staphylococcus aureus Anticuerpos Novilla Respuesta Inmunológica Vacuna Cattle Bovine Mastitis Animal Diseases Antibodies Heifers Immune Response Vaccines ISCOM Matrix |
| url | https://www.sciencedirect.com/science/article/pii/S0165242714002293 http://hdl.handle.net/20.500.12123/2826 https://doi.org/10.1016/j.vetimm.2014.10.004 |
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