Comparison of phenotypic tests for detecting penicillin G resistance with presence of blaZ gene in Staphylococcus aureus isolated from bovine intramammary infections
Few studies have described the relationship between genotypic and phenotypic methods for detecting penicillin resistance in Staphylococcus aureus isolated from bovine intramammary infection (IMI). Six phenotypic methods for penicillinase detection were compared with a genotypic method testing the pr...
| Main Authors: | , , , , , , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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2018
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| Online Access: | https://www.cambridge.org/core/journals/journal-of-dairy-research/article/comparison-of-phenotypic-tests-for-detecting-penicillin-g-resistance-with-presence-of-blaz-gene-in-staphylococcus-aureus-isolated-from-bovine-intramammary-infections/8AEBB8F7BD83F306EE0BF08095AFBCAC http://hdl.handle.net/20.500.12123/2817 https://doi.org/10.1017/S0022029915000242 |
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| author | Russi, Norma B. Maito, Julia Dieser, Silvana Andrea Renna, Maria Sol Signorini, Marcelo Camussone, Cecilia Neder, Veronica Elizabeth Pol, Martín Tirante, Liliana Odierno, Liliana Mónica Calvinho, Luis Fernando |
| author_browse | Calvinho, Luis Fernando Camussone, Cecilia Dieser, Silvana Andrea Maito, Julia Neder, Veronica Elizabeth Odierno, Liliana Mónica Pol, Martín Renna, Maria Sol Russi, Norma B. Signorini, Marcelo Tirante, Liliana |
| author_facet | Russi, Norma B. Maito, Julia Dieser, Silvana Andrea Renna, Maria Sol Signorini, Marcelo Camussone, Cecilia Neder, Veronica Elizabeth Pol, Martín Tirante, Liliana Odierno, Liliana Mónica Calvinho, Luis Fernando |
| author_sort | Russi, Norma B. |
| collection | INTA Digital |
| description | Few studies have described the relationship between genotypic and phenotypic methods for detecting penicillin resistance in Staphylococcus aureus isolated from bovine intramammary infection (IMI). Six phenotypic methods for penicillinase detection were compared with a genotypic method testing the presence of the β-lactamase gene blaZ in Staph. aureus (n = 150) isolated from bovine IMI. Highest sensitivities and specificities were observed for disk diffusion (DD) (93 and 97·4%), minimum inhibitory concentration (MIC) (90·3 and 97·4%), Cefinase™ (85·9 and 97·4%) and Diatabs™ (85·7 and 98·7%). The estimated cut-off points estimated in the present study can be considered close to the ones indicated by CLSI (2013). The molecular detection of blaZ gene is the only method that may indicate the real or potential capacity of producing β-lactamase in Staph. aureus. Considering that from a clinical standpoint a false negative result from a phenotypic test is the most unfavourable situation, a combination of standard DD with Diatabs™ or Cefinase™ should be performed by routine mastitis laboratories to minimise false negative results. |
| format | info:ar-repo/semantics/artículo |
| id | INTA2817 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA28172019-03-21T17:34:39Z Comparison of phenotypic tests for detecting penicillin G resistance with presence of blaZ gene in Staphylococcus aureus isolated from bovine intramammary infections Russi, Norma B. Maito, Julia Dieser, Silvana Andrea Renna, Maria Sol Signorini, Marcelo Camussone, Cecilia Neder, Veronica Elizabeth Pol, Martín Tirante, Liliana Odierno, Liliana Mónica Calvinho, Luis Fernando Staphylococcus aureus Enfermedades de los Animales Ganado Bovino Mastítis Bovina Penicilina Resistencia a los Antibióticos Animal Diseases Cattle Bovine Mastitis Penicillins Resistance to Antibiotics Few studies have described the relationship between genotypic and phenotypic methods for detecting penicillin resistance in Staphylococcus aureus isolated from bovine intramammary infection (IMI). Six phenotypic methods for penicillinase detection were compared with a genotypic method testing the presence of the β-lactamase gene blaZ in Staph. aureus (n = 150) isolated from bovine IMI. Highest sensitivities and specificities were observed for disk diffusion (DD) (93 and 97·4%), minimum inhibitory concentration (MIC) (90·3 and 97·4%), Cefinase™ (85·9 and 97·4%) and Diatabs™ (85·7 and 98·7%). The estimated cut-off points estimated in the present study can be considered close to the ones indicated by CLSI (2013). The molecular detection of blaZ gene is the only method that may indicate the real or potential capacity of producing β-lactamase in Staph. aureus. Considering that from a clinical standpoint a false negative result from a phenotypic test is the most unfavourable situation, a combination of standard DD with Diatabs™ or Cefinase™ should be performed by routine mastitis laboratories to minimise false negative results. EEA Rafaela Fil: Russi, Norma B.. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina Fil: Maito, Julia. Lactodiagnóstico Sur; Argentina Fil: Dieser, Silvana Andrea. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales; Argentina Fil: Renna, Maria Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Signorini, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Camussone, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Neder, Veronica Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Pol, Martín. Lactodiagnóstico Sur; Argentina Fil: Tirante, Liliana. Lactodiagnóstico Sur; Argentina Fil: Odierno, Liliana Mónica. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina 2018-07-18T15:56:03Z 2018-07-18T15:56:03Z 2015-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion https://www.cambridge.org/core/journals/journal-of-dairy-research/article/comparison-of-phenotypic-tests-for-detecting-penicillin-g-resistance-with-presence-of-blaz-gene-in-staphylococcus-aureus-isolated-from-bovine-intramammary-infections/8AEBB8F7BD83F306EE0BF08095AFBCAC http://hdl.handle.net/20.500.12123/2817 0022-0299 1469-7629 https://doi.org/10.1017/S0022029915000242 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Journal of Dairy Research 82 (3) : 317-321 (August 2015) |
| spellingShingle | Staphylococcus aureus Enfermedades de los Animales Ganado Bovino Mastítis Bovina Penicilina Resistencia a los Antibióticos Animal Diseases Cattle Bovine Mastitis Penicillins Resistance to Antibiotics Russi, Norma B. Maito, Julia Dieser, Silvana Andrea Renna, Maria Sol Signorini, Marcelo Camussone, Cecilia Neder, Veronica Elizabeth Pol, Martín Tirante, Liliana Odierno, Liliana Mónica Calvinho, Luis Fernando Comparison of phenotypic tests for detecting penicillin G resistance with presence of blaZ gene in Staphylococcus aureus isolated from bovine intramammary infections |
| title | Comparison of phenotypic tests for detecting penicillin G resistance with presence of blaZ gene in Staphylococcus aureus isolated from bovine intramammary infections |
| title_full | Comparison of phenotypic tests for detecting penicillin G resistance with presence of blaZ gene in Staphylococcus aureus isolated from bovine intramammary infections |
| title_fullStr | Comparison of phenotypic tests for detecting penicillin G resistance with presence of blaZ gene in Staphylococcus aureus isolated from bovine intramammary infections |
| title_full_unstemmed | Comparison of phenotypic tests for detecting penicillin G resistance with presence of blaZ gene in Staphylococcus aureus isolated from bovine intramammary infections |
| title_short | Comparison of phenotypic tests for detecting penicillin G resistance with presence of blaZ gene in Staphylococcus aureus isolated from bovine intramammary infections |
| title_sort | comparison of phenotypic tests for detecting penicillin g resistance with presence of blaz gene in staphylococcus aureus isolated from bovine intramammary infections |
| topic | Staphylococcus aureus Enfermedades de los Animales Ganado Bovino Mastítis Bovina Penicilina Resistencia a los Antibióticos Animal Diseases Cattle Bovine Mastitis Penicillins Resistance to Antibiotics |
| url | https://www.cambridge.org/core/journals/journal-of-dairy-research/article/comparison-of-phenotypic-tests-for-detecting-penicillin-g-resistance-with-presence-of-blaz-gene-in-staphylococcus-aureus-isolated-from-bovine-intramammary-infections/8AEBB8F7BD83F306EE0BF08095AFBCAC http://hdl.handle.net/20.500.12123/2817 https://doi.org/10.1017/S0022029915000242 |
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