Development and assessment of a new cage‐like particle adjuvant
Objectives: To obtain and assess stable cage‐like particles with low surface charge density, which can be prepared using a standardized, economic and scalable method. Methods: To form these nanoparticles, the lipid composition and proportion as well the method were modified in relation to cage‐like...
| Autores principales: | , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: | https://onlinelibrary.wiley.com/doi/abs/10.1111/jphp.12768 http://hdl.handle.net/20.500.12123/2813 https://doi.org/10.1111/jphp.12768 |
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| author | Bertona, Daiana Pujato, Nazarena Bontempi, Iván González, Verónica Doris Guadalupe Cabrera, Gabriel Gustavo Gugliotta, Luis Marcelino Hozbor, Daniela Flavia Nicastro, Alcides Calvinho, Luis Fernando Marcipar, Iván Sergio |
| author_browse | Bertona, Daiana Bontempi, Iván Cabrera, Gabriel Gustavo Calvinho, Luis Fernando González, Verónica Doris Guadalupe Gugliotta, Luis Marcelino Hozbor, Daniela Flavia Marcipar, Iván Sergio Nicastro, Alcides Pujato, Nazarena |
| author_facet | Bertona, Daiana Pujato, Nazarena Bontempi, Iván González, Verónica Doris Guadalupe Cabrera, Gabriel Gustavo Gugliotta, Luis Marcelino Hozbor, Daniela Flavia Nicastro, Alcides Calvinho, Luis Fernando Marcipar, Iván Sergio |
| author_sort | Bertona, Daiana |
| collection | INTA Digital |
| description | Objectives: To obtain and assess stable cage‐like particles with low surface charge density, which can be prepared using a standardized, economic and scalable method.
Methods: To form these nanoparticles, the lipid composition and proportion as well the method were modified in relation to cage‐like particles previously described elsewhere. Bovine albumin was used to compare ISPA performance with that of other adjuvants in mice and to assess stability. Adjuvant efficacy was analysed using a mouse model of Trypanosoma cruzi infection, which shows protection against an intracellular infection that needs a strong cellular response.
Key findings: The new particles were better in terms of level, kinetics and profile of humoral responses than Freund Adjuvant, aluminium hydroxide and Montanide TM ISA 206; they also tended to improve ISCOMATRIX™ performance. Particle size and adjuvant performance were conserved during the 6‐month period assessed after preparation. In the model of Trypanosoma cruzi infection, mice immunized with ISPA and trans‐sialidase developed high protection.
Conclusions: The obtained nanoparticles were stable and outperformed the other assessed adjuvants in joining together the capacity of most adjuvants to enhance the immune response against specific antigen, to reduce the number of doses, to homogenize the response between individuals and to reach a balanced TH1/TH2 response. |
| format | Artículo |
| id | INTA2813 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA28132018-07-18T14:22:50Z Development and assessment of a new cage‐like particle adjuvant Bertona, Daiana Pujato, Nazarena Bontempi, Iván González, Verónica Doris Guadalupe Cabrera, Gabriel Gustavo Gugliotta, Luis Marcelino Hozbor, Daniela Flavia Nicastro, Alcides Calvinho, Luis Fernando Marcipar, Iván Sergio Ganado Bovino Trypanosoma cruzi Coadyuvantes Respuesta Inmunológica Cattle Adjuvants Immune Response Objectives: To obtain and assess stable cage‐like particles with low surface charge density, which can be prepared using a standardized, economic and scalable method. Methods: To form these nanoparticles, the lipid composition and proportion as well the method were modified in relation to cage‐like particles previously described elsewhere. Bovine albumin was used to compare ISPA performance with that of other adjuvants in mice and to assess stability. Adjuvant efficacy was analysed using a mouse model of Trypanosoma cruzi infection, which shows protection against an intracellular infection that needs a strong cellular response. Key findings: The new particles were better in terms of level, kinetics and profile of humoral responses than Freund Adjuvant, aluminium hydroxide and Montanide TM ISA 206; they also tended to improve ISCOMATRIX™ performance. Particle size and adjuvant performance were conserved during the 6‐month period assessed after preparation. In the model of Trypanosoma cruzi infection, mice immunized with ISPA and trans‐sialidase developed high protection. Conclusions: The obtained nanoparticles were stable and outperformed the other assessed adjuvants in joining together the capacity of most adjuvants to enhance the immune response against specific antigen, to reduce the number of doses, to homogenize the response between individuals and to reach a balanced TH1/TH2 response. EEA Rafaela Fil: Bertona, Daiana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina Fil: Pujato, Nazarena. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina Fil: Bontempi, Iván. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina Fil: González, Verónica Doris Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina. Universidad Nacional del Litoral; Argentina Fil: Cabrera, Gabriel Gustavo. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gugliotta, Luis Marcelino. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina Fil: Hozbor, Daniela Flavia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Laboratorio VacSal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Nicastro, Alcides. LIPOMIZE SRL Santa Fe; Argentina Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Marcipar, Ivan Sergio. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2018-07-18T14:14:26Z 2018-07-18T14:14:26Z 2017-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://onlinelibrary.wiley.com/doi/abs/10.1111/jphp.12768 http://hdl.handle.net/20.500.12123/2813 0022-3573 2042-7158 https://doi.org/10.1111/jphp.12768 eng info:eu-repo/semantics/restrictedAccess application/pdf Journal of Pharmacy and Pharmacology 69 (10) : 1293-1303 (October 2017) |
| spellingShingle | Ganado Bovino Trypanosoma cruzi Coadyuvantes Respuesta Inmunológica Cattle Adjuvants Immune Response Bertona, Daiana Pujato, Nazarena Bontempi, Iván González, Verónica Doris Guadalupe Cabrera, Gabriel Gustavo Gugliotta, Luis Marcelino Hozbor, Daniela Flavia Nicastro, Alcides Calvinho, Luis Fernando Marcipar, Iván Sergio Development and assessment of a new cage‐like particle adjuvant |
| title | Development and assessment of a new cage‐like particle adjuvant |
| title_full | Development and assessment of a new cage‐like particle adjuvant |
| title_fullStr | Development and assessment of a new cage‐like particle adjuvant |
| title_full_unstemmed | Development and assessment of a new cage‐like particle adjuvant |
| title_short | Development and assessment of a new cage‐like particle adjuvant |
| title_sort | development and assessment of a new cage like particle adjuvant |
| topic | Ganado Bovino Trypanosoma cruzi Coadyuvantes Respuesta Inmunológica Cattle Adjuvants Immune Response |
| url | https://onlinelibrary.wiley.com/doi/abs/10.1111/jphp.12768 http://hdl.handle.net/20.500.12123/2813 https://doi.org/10.1111/jphp.12768 |
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