Exploring the diversity of the root-associated microbiome of Ilex paraguariensis St. Hil. (Yerba Mate)
Ilex paraguariensis St. Hil. (Yerba Mate) is an important crop for which a decrease in yields associated to unsustainable agricultural practices is well documented. The aim of this study is to investigate the diversity of bacteria and fungi inhabiting roots of Yerba Mate. This is an important pre-re...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/2791 http://dx.doi.org/10.1016/j.apsoil.2016.09.013 |
| _version_ | 1855483120968531968 |
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| author | Bergottini, Veronica M. Hervé, Vincent Sosa, Domingo Alberto Otegui, Mónica B. Zapata, Pedro Darío Junier, Pilar |
| author_browse | Bergottini, Veronica M. Hervé, Vincent Junier, Pilar Otegui, Mónica B. Sosa, Domingo Alberto Zapata, Pedro Darío |
| author_facet | Bergottini, Veronica M. Hervé, Vincent Sosa, Domingo Alberto Otegui, Mónica B. Zapata, Pedro Darío Junier, Pilar |
| author_sort | Bergottini, Veronica M. |
| collection | INTA Digital |
| description | Ilex paraguariensis St. Hil. (Yerba Mate) is an important crop for which a decrease in yields associated to unsustainable agricultural practices is well documented. The aim of this study is to investigate the diversity of bacteria and fungi inhabiting roots of Yerba Mate. This is an important pre-requisite for the use of microorganisms inhabiting roots to modulate plant nutrition and health as an ecologically friendly agricultural alternative for this crop. The diversity of the root-associated microbiome from eleven plantations with different agricultural practices was analyzed by high throughput sequencing of the 16S rRNA gene as a bacterial marker, whereas the fungal communities were targeted by amplifying the ITS region of the ribosomal RNA gene cluster. A comparison of the bacterial and fungal communities between plantation sites and cultivation practices was made to address the major factors contributing to the structure of the root microbiome of this crop. Operational taxonomic units (OTUs) related to well-known plant growth promoting bacteria such as Burkholderia, Bradyrhizobium, Weissella, Enterobacter and Rhizobium were detected. Those might constitute targets for future enrichment efforts of plant growth promoting clades. The analysis of the fungal community composition demonstrated that arbuscular mycorrhizae colonize Yerba Mate roots, and that the frequency of this group is favored in degraded soils. The detection of other groups harboring potential phytopathogens might help to broaden the understanding of the ailments affecting this crop. This study provides the
first description of the root-associated microbiome of Yerba Mate and constitutes a stepping-stone towards harnessing the role of microbes in the sustainable cultivation of this crop. |
| format | Artículo |
| id | INTA2791 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA27912018-07-16T11:24:02Z Exploring the diversity of the root-associated microbiome of Ilex paraguariensis St. Hil. (Yerba Mate) Bergottini, Veronica M. Hervé, Vincent Sosa, Domingo Alberto Otegui, Mónica B. Zapata, Pedro Darío Junier, Pilar Ilex Paraguariensis Mate Microorganismos Raíces Burkholderiaceae Mycorrhizae Rhizobacteria Rizobacterias Roots Microorganisms Ilex paraguariensis St. Hil. (Yerba Mate) is an important crop for which a decrease in yields associated to unsustainable agricultural practices is well documented. The aim of this study is to investigate the diversity of bacteria and fungi inhabiting roots of Yerba Mate. This is an important pre-requisite for the use of microorganisms inhabiting roots to modulate plant nutrition and health as an ecologically friendly agricultural alternative for this crop. The diversity of the root-associated microbiome from eleven plantations with different agricultural practices was analyzed by high throughput sequencing of the 16S rRNA gene as a bacterial marker, whereas the fungal communities were targeted by amplifying the ITS region of the ribosomal RNA gene cluster. A comparison of the bacterial and fungal communities between plantation sites and cultivation practices was made to address the major factors contributing to the structure of the root microbiome of this crop. Operational taxonomic units (OTUs) related to well-known plant growth promoting bacteria such as Burkholderia, Bradyrhizobium, Weissella, Enterobacter and Rhizobium were detected. Those might constitute targets for future enrichment efforts of plant growth promoting clades. The analysis of the fungal community composition demonstrated that arbuscular mycorrhizae colonize Yerba Mate roots, and that the frequency of this group is favored in degraded soils. The detection of other groups harboring potential phytopathogens might help to broaden the understanding of the ailments affecting this crop. This study provides the first description of the root-associated microbiome of Yerba Mate and constitutes a stepping-stone towards harnessing the role of microbes in the sustainable cultivation of this crop. Fil: Bergottini, Veronica M. University of Neuchâtel. Laboratory of Microbiology; Suiza Fil: Hervé, Vincent. University of Neuchâtel. Laboratory of Microbiology; Suiza. University of Lausanne. Institute of Earth Surface Dynamics. Laboratory of Biogeosciences; Suiza Fil: Sosa, Domingo Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cerro Azul; Argentina Fil: Otegui, Mónica B. Universidad Nacional de Misiones. Instituto de Biotecnología Misiones; Argentina Fil: Zapata, Pedro Darío. Universidad Nacional de Misiones. Instituto de Biotecnología Misiones; Argentina Fil: Junier, Pilar. University of Neuchâtel. Laboratory of Microbiology; Suiza 2018-07-13T15:55:17Z 2018-07-13T15:55:17Z 2017 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/2791 0929-1393 http://dx.doi.org/10.1016/j.apsoil.2016.09.013 eng info:eu-repo/semantics/restrictedAccess application/pdf Applied soil ecology 109 : 23–31. (2017) |
| spellingShingle | Ilex Paraguariensis Mate Microorganismos Raíces Burkholderiaceae Mycorrhizae Rhizobacteria Rizobacterias Roots Microorganisms Bergottini, Veronica M. Hervé, Vincent Sosa, Domingo Alberto Otegui, Mónica B. Zapata, Pedro Darío Junier, Pilar Exploring the diversity of the root-associated microbiome of Ilex paraguariensis St. Hil. (Yerba Mate) |
| title | Exploring the diversity of the root-associated microbiome of Ilex paraguariensis St. Hil. (Yerba Mate) |
| title_full | Exploring the diversity of the root-associated microbiome of Ilex paraguariensis St. Hil. (Yerba Mate) |
| title_fullStr | Exploring the diversity of the root-associated microbiome of Ilex paraguariensis St. Hil. (Yerba Mate) |
| title_full_unstemmed | Exploring the diversity of the root-associated microbiome of Ilex paraguariensis St. Hil. (Yerba Mate) |
| title_short | Exploring the diversity of the root-associated microbiome of Ilex paraguariensis St. Hil. (Yerba Mate) |
| title_sort | exploring the diversity of the root associated microbiome of ilex paraguariensis st hil yerba mate |
| topic | Ilex Paraguariensis Mate Microorganismos Raíces Burkholderiaceae Mycorrhizae Rhizobacteria Rizobacterias Roots Microorganisms |
| url | http://hdl.handle.net/20.500.12123/2791 http://dx.doi.org/10.1016/j.apsoil.2016.09.013 |
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