Matrix interference evaluation employing GC and LC coupled to triple quadrupole tandem mass spectrometry
Gas and liquid chromatography coupled to triple quadrupole tandem mass spectrometry are currently the most powerful tools employed for the routine analysis of pesticide residues in food control laboratories. However, whatever the multiresidue extraction method, there will be a residual matrix effect...
| Autores principales: | , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/2777 http://dx.doi.org/10.1016/j.talanta.2017.05.068 |
| _version_ | 1855035024472014848 |
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| author | Uclés, Samanta Lozano Fernández, A.B. Sosa, Alexis Lionel Parrilla Vázquez, Piedad Valverde García, Antonio Rodríguez Fernández-Alba, Amadeo |
| author_browse | Lozano Fernández, A.B. Parrilla Vázquez, Piedad Rodríguez Fernández-Alba, Amadeo Sosa, Alexis Lionel Uclés, Samanta Valverde García, Antonio |
| author_facet | Uclés, Samanta Lozano Fernández, A.B. Sosa, Alexis Lionel Parrilla Vázquez, Piedad Valverde García, Antonio Rodríguez Fernández-Alba, Amadeo |
| author_sort | Uclés, Samanta |
| collection | INTA Digital |
| description | Gas and liquid chromatography coupled to triple quadrupole tandem mass spectrometry are currently the most powerful tools employed for the routine analysis of pesticide residues in food control laboratories. However, whatever the multiresidue extraction method, there will be a residual matrix effect making it difficult to identify/quantify some specific compounds in certain cases. Two main effects stand out: (i) co-elution with isobaric
matrix interferents, which can be a major drawback for unequivocal identification, and therefore false negative detections, and (ii) signal suppression/enhancement, commonly called the “matrix effect”, which may cause serious problems including inaccurate quantitation, low analyte detectability and increased method uncertainty.
The aim of this analytical study is to provide a framework for evaluating the maximum expected errors associated with the matrix effects. The worst-case study contrived to give an estimation of the extreme errors caused by matrix effects when extraction/determination protocols are applied in routine multiresidue analysis. Twenty-five different blank matrices extracted with the four most common extraction methods used in routine analysis (citrate QuEChERS with/without PSA clean-up, ethyl acetate and the Dutch mini-Luke “NL” methods) were evaluated by both GC-QqQ-MS/MS and LC-QqQ-MS/MS. The results showed that the presence of matrix compounds with isobaric transitions to target pesticides was higher in GC than under LC in the experimental conditions tested.
In a second study, the number of “potential” false negatives was evaluated. For that, ten matrices with higher percentages of natural interfering components were checked. Additionally, the results showed that for more than 90% of the cases, pesticide quantification was not affected by matrix-matched standard calibration when an interferent was kept constant along the calibration curve. The error in quantification depended on the concentration level. In a third study, the “matrix effect” was evaluated for each commodity/extraction method. Results showed 44% of cases with suppression/enhancement for LC and 93% of cases with enhancement for GC. |
| format | info:ar-repo/semantics/artículo |
| id | INTA2777 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA27772018-07-26T15:41:45Z Matrix interference evaluation employing GC and LC coupled to triple quadrupole tandem mass spectrometry Uclés, Samanta Lozano Fernández, A.B. Sosa, Alexis Lionel Parrilla Vázquez, Piedad Valverde García, Antonio Rodríguez Fernández-Alba, Amadeo Frutas Hortalizas Residuos de Plaguicidas Espectrometría de Masas Mass Spectrometry Pesticide Residues Vegetables Fruits LC-MS/MS GC-MS/MS Gas and liquid chromatography coupled to triple quadrupole tandem mass spectrometry are currently the most powerful tools employed for the routine analysis of pesticide residues in food control laboratories. However, whatever the multiresidue extraction method, there will be a residual matrix effect making it difficult to identify/quantify some specific compounds in certain cases. Two main effects stand out: (i) co-elution with isobaric matrix interferents, which can be a major drawback for unequivocal identification, and therefore false negative detections, and (ii) signal suppression/enhancement, commonly called the “matrix effect”, which may cause serious problems including inaccurate quantitation, low analyte detectability and increased method uncertainty. The aim of this analytical study is to provide a framework for evaluating the maximum expected errors associated with the matrix effects. The worst-case study contrived to give an estimation of the extreme errors caused by matrix effects when extraction/determination protocols are applied in routine multiresidue analysis. Twenty-five different blank matrices extracted with the four most common extraction methods used in routine analysis (citrate QuEChERS with/without PSA clean-up, ethyl acetate and the Dutch mini-Luke “NL” methods) were evaluated by both GC-QqQ-MS/MS and LC-QqQ-MS/MS. The results showed that the presence of matrix compounds with isobaric transitions to target pesticides was higher in GC than under LC in the experimental conditions tested. In a second study, the number of “potential” false negatives was evaluated. For that, ten matrices with higher percentages of natural interfering components were checked. Additionally, the results showed that for more than 90% of the cases, pesticide quantification was not affected by matrix-matched standard calibration when an interferent was kept constant along the calibration curve. The error in quantification depended on the concentration level. In a third study, the “matrix effect” was evaluated for each commodity/extraction method. Results showed 44% of cases with suppression/enhancement for LC and 93% of cases with enhancement for GC. EEA Concordia Fil: Uclés, Samanta. Universidad de Almería. Departamento de Química y Física. Campus de Excelencia Internacional en Agroalimentación; España. European Union Reference Laboratory for Pesticide Residues in Fruit and Vegetables; España Fil: Lozano Fernández, A.B. Universidad de Almería. Departamento de Química y Física. Campus de Excelencia Internacional en Agroalimentación; España. European Union Reference Laboratory for Pesticide Residues in Fruit and Vegetables; España Fil: Sosa, Alexis Lionel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Parrilla Vázquez, Piedad. Universidad de Almería. Departamento de Química y Física. Campus de Excelencia Internacional en Agroalimentación; España. European Union Reference Laboratory for Pesticide Residues in Fruit and Vegetables; España Fil: Valverde García, Antonio. Universidad de Almería. Departamento de Química y Física. Campus de Excelencia Internacional en Agroalimentación; España. European Union Reference Laboratory for Pesticide Residues in Fruit and Vegetables; España Fil: Rodríguez Fernández-Alba, Amadeo. Universidad de Almería. Departamento de Química y Física. Campus de Excelencia Internacional en Agroalimentación; España. European Union Reference Laboratory for Pesticide Residues in Fruit and Vegetables; España 2018-07-12T14:35:52Z 2018-07-12T14:35:52Z 2017 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/2777 0039-9140 http://dx.doi.org/10.1016/j.talanta.2017.05.068 eng info:eu-repo/semantics/restrictedAccess application/pdf Talanta 174 : 72–81. (2017) |
| spellingShingle | Frutas Hortalizas Residuos de Plaguicidas Espectrometría de Masas Mass Spectrometry Pesticide Residues Vegetables Fruits LC-MS/MS GC-MS/MS Uclés, Samanta Lozano Fernández, A.B. Sosa, Alexis Lionel Parrilla Vázquez, Piedad Valverde García, Antonio Rodríguez Fernández-Alba, Amadeo Matrix interference evaluation employing GC and LC coupled to triple quadrupole tandem mass spectrometry |
| title | Matrix interference evaluation employing GC and LC coupled to triple quadrupole tandem mass spectrometry |
| title_full | Matrix interference evaluation employing GC and LC coupled to triple quadrupole tandem mass spectrometry |
| title_fullStr | Matrix interference evaluation employing GC and LC coupled to triple quadrupole tandem mass spectrometry |
| title_full_unstemmed | Matrix interference evaluation employing GC and LC coupled to triple quadrupole tandem mass spectrometry |
| title_short | Matrix interference evaluation employing GC and LC coupled to triple quadrupole tandem mass spectrometry |
| title_sort | matrix interference evaluation employing gc and lc coupled to triple quadrupole tandem mass spectrometry |
| topic | Frutas Hortalizas Residuos de Plaguicidas Espectrometría de Masas Mass Spectrometry Pesticide Residues Vegetables Fruits LC-MS/MS GC-MS/MS |
| url | http://hdl.handle.net/20.500.12123/2777 http://dx.doi.org/10.1016/j.talanta.2017.05.068 |
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