Vaginal mucus from ewes treated with progestogen sponges affects quality of ram spermatozoa
The use of intravaginal sponges (IS) to synchronize estrous onset in ewes provokes vaginitis, an increase in the vaginal bacterial load, and growth of bacterial species that are not present during spontaneous estrous behavior. The objective of the study was to compare the functional sperm paramet...
| Autores principales: | , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/2762 http://dx.doi.org/10.1016/j.theriogenology.2015.10.033 |
| _version_ | 1855035021683851264 |
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| author | Manes, Jorgelina Rios, Glenda Laura Fiorentino, María Andrea Ungerfeld, Rodolfo |
| author_browse | Fiorentino, María Andrea Manes, Jorgelina Rios, Glenda Laura Ungerfeld, Rodolfo |
| author_facet | Manes, Jorgelina Rios, Glenda Laura Fiorentino, María Andrea Ungerfeld, Rodolfo |
| author_sort | Manes, Jorgelina |
| collection | INTA Digital |
| description | The use of intravaginal sponges (IS) to synchronize estrous onset in ewes provokes vaginitis,
an increase in the vaginal bacterial load, and growth of bacterial species that are not
present during spontaneous estrous behavior. The objective of the study was to compare
the functional sperm parameters after incubating it with mucus collected from the vagina
of ewes during spontaneous estrus or estrous synchronized with IS. Pooled spermatozoa
were co-incubated with: (1) vaginal mucus collected from ewes in spontaneous estrus; (2)
vaginal mucus collected from ewes in estrus pretreated with progestogen-impregnated IS;
(3) synthetic mucus; and (4) medium without mucus as a control group. Sperm samples
were evaluated after incubating it for 30 and 90 minutes. The number of colony-forming
units (CFUs/mL), pH, and osmolality were greater in the mucus collected from ewes
treated with IS than from those untreated (P ¼ 0.046; P < 0.0001, and P < 0.0001,
respectively). The percentage of sperm with progressive motility was lower after incubation
with vaginal mucus collected from estrous ewes treated with IS than in the other three
treatments both, 30 and 90 minutes after incubation (P ¼ 0.0009 and P < 0.0001,
respectively). The sample incubated for 30 minutes with mucus from ewes treated with IS
had a lower percentage of sperm with intact plasma membrane than all the other treatments
(P < 0.0001). The percentage of sperm with functional membrane was significantly
lower in the sample incubated for 30 minutes with vaginal mucus from ewes treated with
IS than in the other three treatments (P < 0.0001). After 90 minutes, the percentage was
still lower than that in the sample collected from ewes during their spontaneous estrus
(P ¼ 0.0005). The lowest percentages of sperm with acrosome damage were observed in
sperm incubated with mucus collected from sheep in spontaneous estrus for 30 and
90 minutes (P < 0.0001 and P ¼ 0.008, respectively). The percentage of apoptotic spermatozoa
was greater in samples incubated during 30 minutes with vaginal mucus
collected from ewes treated with IS than in the other three groups (P ¼ 0.0005). The
functionality and the viability of ram sperm is negatively affected by the cervical mucus of
ewes pretreated with progestagen-impregnated IS used in estrous synchronization treatments.
This may partially explain the decrease in conception rate obtained with treatments
with IS. |
| format | info:ar-repo/semantics/artículo |
| id | INTA2762 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA27622018-07-11T12:32:10Z Vaginal mucus from ewes treated with progestogen sponges affects quality of ram spermatozoa Manes, Jorgelina Rios, Glenda Laura Fiorentino, María Andrea Ungerfeld, Rodolfo Sincronización del Celo Oveja Vaginitis Espermatozoo Morueco Progestagenos Progestogens Rams Spermatozoa Ewes Oestrus Synchronization Reproduction Control Reproducción Dirigida Carnero Esponjas Intravaginales Intravaginal Sponge The use of intravaginal sponges (IS) to synchronize estrous onset in ewes provokes vaginitis, an increase in the vaginal bacterial load, and growth of bacterial species that are not present during spontaneous estrous behavior. The objective of the study was to compare the functional sperm parameters after incubating it with mucus collected from the vagina of ewes during spontaneous estrus or estrous synchronized with IS. Pooled spermatozoa were co-incubated with: (1) vaginal mucus collected from ewes in spontaneous estrus; (2) vaginal mucus collected from ewes in estrus pretreated with progestogen-impregnated IS; (3) synthetic mucus; and (4) medium without mucus as a control group. Sperm samples were evaluated after incubating it for 30 and 90 minutes. The number of colony-forming units (CFUs/mL), pH, and osmolality were greater in the mucus collected from ewes treated with IS than from those untreated (P ¼ 0.046; P < 0.0001, and P < 0.0001, respectively). The percentage of sperm with progressive motility was lower after incubation with vaginal mucus collected from estrous ewes treated with IS than in the other three treatments both, 30 and 90 minutes after incubation (P ¼ 0.0009 and P < 0.0001, respectively). The sample incubated for 30 minutes with mucus from ewes treated with IS had a lower percentage of sperm with intact plasma membrane than all the other treatments (P < 0.0001). The percentage of sperm with functional membrane was significantly lower in the sample incubated for 30 minutes with vaginal mucus from ewes treated with IS than in the other three treatments (P < 0.0001). After 90 minutes, the percentage was still lower than that in the sample collected from ewes during their spontaneous estrus (P ¼ 0.0005). The lowest percentages of sperm with acrosome damage were observed in sperm incubated with mucus collected from sheep in spontaneous estrus for 30 and 90 minutes (P < 0.0001 and P ¼ 0.008, respectively). The percentage of apoptotic spermatozoa was greater in samples incubated during 30 minutes with vaginal mucus collected from ewes treated with IS than in the other three groups (P ¼ 0.0005). The functionality and the viability of ram sperm is negatively affected by the cervical mucus of ewes pretreated with progestagen-impregnated IS used in estrous synchronization treatments. This may partially explain the decrease in conception rate obtained with treatments with IS. EEA Balcarce Fil: Manes, Jorgelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina. Universidad de la República. Facultad de Veterinaria. Departamento de Fisiología; Uruguay Fil: Rios, Glenda Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina Fil: Fiorentino, María Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Laboratorio de Bacteriología; Argentina Fil: Ungerfeld, Rodolfo. Universidad de la República. Facultad de Veterinaria. Departamento de Fisiología; Uruguay 2018-07-11T12:23:41Z 2018-07-11T12:23:41Z 2016 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/2762 0093-691X http://dx.doi.org/10.1016/j.theriogenology.2015.10.033 eng info:eu-repo/semantics/restrictedAccess application/pdf Theriogenology 85 : 856–861. (2016) |
| spellingShingle | Sincronización del Celo Oveja Vaginitis Espermatozoo Morueco Progestagenos Progestogens Rams Spermatozoa Ewes Oestrus Synchronization Reproduction Control Reproducción Dirigida Carnero Esponjas Intravaginales Intravaginal Sponge Manes, Jorgelina Rios, Glenda Laura Fiorentino, María Andrea Ungerfeld, Rodolfo Vaginal mucus from ewes treated with progestogen sponges affects quality of ram spermatozoa |
| title | Vaginal mucus from ewes treated with progestogen sponges affects quality of ram spermatozoa |
| title_full | Vaginal mucus from ewes treated with progestogen sponges affects quality of ram spermatozoa |
| title_fullStr | Vaginal mucus from ewes treated with progestogen sponges affects quality of ram spermatozoa |
| title_full_unstemmed | Vaginal mucus from ewes treated with progestogen sponges affects quality of ram spermatozoa |
| title_short | Vaginal mucus from ewes treated with progestogen sponges affects quality of ram spermatozoa |
| title_sort | vaginal mucus from ewes treated with progestogen sponges affects quality of ram spermatozoa |
| topic | Sincronización del Celo Oveja Vaginitis Espermatozoo Morueco Progestagenos Progestogens Rams Spermatozoa Ewes Oestrus Synchronization Reproduction Control Reproducción Dirigida Carnero Esponjas Intravaginales Intravaginal Sponge |
| url | http://hdl.handle.net/20.500.12123/2762 http://dx.doi.org/10.1016/j.theriogenology.2015.10.033 |
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