Changes in the vaginal microbiota in ewes after insertion of intravaginal sponges at different stages of the oestrous cycle
The aims of this study were to determine if the ewe’ vaginal bacterial load differs according to the stage of the oestrous cycle; and thus, if the stage of the oestrous cycle when intravaginal sponges (IS) used for oestrous synchronisation are inserted influences the vaginal bacterial biota presen...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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2018
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| Online Access: | http://hdl.handle.net/20.500.12123/2759 https://doi.org/10.1016/j.livsci.2017.11.023 |
| _version_ | 1855035021128105984 |
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| author | Manes, Jorgelina Fiorentino, María Andrea San Martino, Silvina Ungerfeld, Rodolfo |
| author_browse | Fiorentino, María Andrea Manes, Jorgelina San Martino, Silvina Ungerfeld, Rodolfo |
| author_facet | Manes, Jorgelina Fiorentino, María Andrea San Martino, Silvina Ungerfeld, Rodolfo |
| author_sort | Manes, Jorgelina |
| collection | INTA Digital |
| description | The aims of this study were to determine if the ewe’ vaginal bacterial load differs according to the stage of the
oestrous cycle; and thus, if the stage of the oestrous cycle when intravaginal sponges (IS) used for oestrous
synchronisation are inserted influences the vaginal bacterial biota present at sponge withdrawal. During the
breeding season, 45 Texel ewes were detected in oestrus and randomly allocated to one of three treatments of 15
ewes each according to the stage of the oestrous cycle when the IS were inserted. Intravaginal sponges were
inserted: the day of oestrus (GOes), or 2 (GMet) or 8 (GDie) days after oestrus. Samples for bacterial analysis
were collected the day of IS insertion (Day 0), at sponge withdrawal (Day 12), 24 h (Day 13) and 48 h (Day 14)
after sponge withdrawal. Vaginal bacteria amount increased at sponge withdrawal in all treatment (p<0.0001).
The number of different types of colonies was greater in GMet group than in GDie at sponge insertion (p<0.05),
without difference with GOes. At sponge insertion, the presence of Staphylococcus spp. was greater in GMet than
in GOe and GDi (p<0.05); the presence of Trueperella pyogenes and Haemophilus somni was greater after sponge
withdrawal than before sponge insertion (p<0.001), but without difference between days 12, 13 and 14.
Overall, we concluded that in ewes the normal vaginal bacterial load differs according to the stage of the
oestrous cycle. The continuous presence of the IS homogenised the vaginal bacterial microbiota at withdrawal,
with only slight and transient differences observed on day 13. |
| format | info:ar-repo/semantics/artículo |
| id | INTA2759 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA27592018-07-10T18:37:04Z Changes in the vaginal microbiota in ewes after insertion of intravaginal sponges at different stages of the oestrous cycle Manes, Jorgelina Fiorentino, María Andrea San Martino, Silvina Ungerfeld, Rodolfo Flora Microbiana Vagina Sincronización del Celo Ciclo Estral Vaginitis Oveja Ewes Oestrous Cycle Oestrus Synchronization Microbial Flora Microbiota Vaginal Esponjas Intravaginales Intravaginal Sponges The aims of this study were to determine if the ewe’ vaginal bacterial load differs according to the stage of the oestrous cycle; and thus, if the stage of the oestrous cycle when intravaginal sponges (IS) used for oestrous synchronisation are inserted influences the vaginal bacterial biota present at sponge withdrawal. During the breeding season, 45 Texel ewes were detected in oestrus and randomly allocated to one of three treatments of 15 ewes each according to the stage of the oestrous cycle when the IS were inserted. Intravaginal sponges were inserted: the day of oestrus (GOes), or 2 (GMet) or 8 (GDie) days after oestrus. Samples for bacterial analysis were collected the day of IS insertion (Day 0), at sponge withdrawal (Day 12), 24 h (Day 13) and 48 h (Day 14) after sponge withdrawal. Vaginal bacteria amount increased at sponge withdrawal in all treatment (p<0.0001). The number of different types of colonies was greater in GMet group than in GDie at sponge insertion (p<0.05), without difference with GOes. At sponge insertion, the presence of Staphylococcus spp. was greater in GMet than in GOe and GDi (p<0.05); the presence of Trueperella pyogenes and Haemophilus somni was greater after sponge withdrawal than before sponge insertion (p<0.001), but without difference between days 12, 13 and 14. Overall, we concluded that in ewes the normal vaginal bacterial load differs according to the stage of the oestrous cycle. The continuous presence of the IS homogenised the vaginal bacterial microbiota at withdrawal, with only slight and transient differences observed on day 13. Fil: Manes, Jorgelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina. Universidad de la República. Facultad de Veterinaria. Departamento de Fisiología; Uruguay Fil: Fiorentino, María Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Laboratorio de Bacteriología; Argentina Fil: San Martino, Silvina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias. Departamento de Introducción a las Ciencias Agrarias. Estadística y Diseño Experimental; Argentina Fil: Ungerfeld, Rodolfo. Universidad de la República. Facultad de Veterinaria. Departamento de Fisiología; Uruguay 2018-07-10T18:26:28Z 2018-07-10T18:26:28Z 2018 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/2759 1871-1413 https://doi.org/10.1016/j.livsci.2017.11.023 eng info:eu-repo/semantics/restrictedAccess application/pdf Livestock science 208 : 55–59. (2018) |
| spellingShingle | Flora Microbiana Vagina Sincronización del Celo Ciclo Estral Vaginitis Oveja Ewes Oestrous Cycle Oestrus Synchronization Microbial Flora Microbiota Vaginal Esponjas Intravaginales Intravaginal Sponges Manes, Jorgelina Fiorentino, María Andrea San Martino, Silvina Ungerfeld, Rodolfo Changes in the vaginal microbiota in ewes after insertion of intravaginal sponges at different stages of the oestrous cycle |
| title | Changes in the vaginal microbiota in ewes after insertion of intravaginal sponges at different stages of the oestrous cycle |
| title_full | Changes in the vaginal microbiota in ewes after insertion of intravaginal sponges at different stages of the oestrous cycle |
| title_fullStr | Changes in the vaginal microbiota in ewes after insertion of intravaginal sponges at different stages of the oestrous cycle |
| title_full_unstemmed | Changes in the vaginal microbiota in ewes after insertion of intravaginal sponges at different stages of the oestrous cycle |
| title_short | Changes in the vaginal microbiota in ewes after insertion of intravaginal sponges at different stages of the oestrous cycle |
| title_sort | changes in the vaginal microbiota in ewes after insertion of intravaginal sponges at different stages of the oestrous cycle |
| topic | Flora Microbiana Vagina Sincronización del Celo Ciclo Estral Vaginitis Oveja Ewes Oestrous Cycle Oestrus Synchronization Microbial Flora Microbiota Vaginal Esponjas Intravaginales Intravaginal Sponges |
| url | http://hdl.handle.net/20.500.12123/2759 https://doi.org/10.1016/j.livsci.2017.11.023 |
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