Use of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi
Background: Asian soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. & Syd., is one of the main diseases affecting soybean and has been reported as one of the most economically important fungal pathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered when deve...
| Autores principales: | , , , , , , , , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S0717345815001268 http://hdl.handle.net/20.500.12123/2721 https://doi.org/10.1016/j.ejbt.2015.06.007 |
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| author | Rocha, Carla Maria Lourdes Vellicce, Gabriel Ricardo Garcia, Maria Gabriela Pardo, Esteban Mariano Racedo, Josefina Perera, María Francisca De Lucia, Adrían Darío Gilli, Javier Ramon Bogado, Noelia Bonnecarrére, Victoria German, Silvia Marcelino, Francismar Ledesma, Fernando Reznikov, Sebastian Ploper, Leonardo Daniel Welin, Bjorn Castagnaro, Atilio Pedro |
| author_browse | Bogado, Noelia Bonnecarrére, Victoria Castagnaro, Atilio Pedro De Lucia, Adrían Darío Garcia, Maria Gabriela German, Silvia Gilli, Javier Ramon Ledesma, Fernando Marcelino, Francismar Pardo, Esteban Mariano Perera, María Francisca Ploper, Leonardo Daniel Racedo, Josefina Reznikov, Sebastian Rocha, Carla Maria Lourdes Vellicce, Gabriel Ricardo Welin, Bjorn |
| author_facet | Rocha, Carla Maria Lourdes Vellicce, Gabriel Ricardo Garcia, Maria Gabriela Pardo, Esteban Mariano Racedo, Josefina Perera, María Francisca De Lucia, Adrían Darío Gilli, Javier Ramon Bogado, Noelia Bonnecarrére, Victoria German, Silvia Marcelino, Francismar Ledesma, Fernando Reznikov, Sebastian Ploper, Leonardo Daniel Welin, Bjorn Castagnaro, Atilio Pedro |
| author_sort | Rocha, Carla Maria Lourdes |
| collection | INTA Digital |
| description | Background: Asian soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. & Syd., is one of the main diseases affecting soybean and has been reported as one of the most economically important fungal pathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered when developing resistance breeding strategies. We aimed to analyze the genetic diversity of P. pachyrhizi combining simple sampling with a powerful and reproducible molecular technique.
Results: We employed Amplified Fragment Length Polymorphism (AFLP) technique for the amplification of P. pachyrhizi DNA extracted from naturally SBR-infected plants from 23 production fields. From a total of 1919 markers obtained, 77% were polymorphic. The high percentage of polymorphism and the Nei's genetic diversity coefficient (0.22) indicated high pathogen diversity. Analysis of molecular variance showed higher genetic variation within countries than among them. Temporal analysis showed a higher genetic variation within a year than between years. Cluster, phylogenetic and principal co-ordinate analysis showed that samples group by year of collection and then by country sampled.
Conclusions: The study proposed combining a simple collection of urediniospore with a subsequent analysis by AFLP was useful to examine the molecular polymorphism of samples of P. pachyrhizi collected and might have a significant contribution to the knowledge of its genetic diversity. Also, AFLP analysis is an important and potent molecular tool for the study of genetic diversity and could be useful to carry out wider genetic diversity studies. |
| format | info:ar-repo/semantics/artículo |
| id | INTA2721 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA27212018-07-04T13:43:27Z Use of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi Rocha, Carla Maria Lourdes Vellicce, Gabriel Ricardo Garcia, Maria Gabriela Pardo, Esteban Mariano Racedo, Josefina Perera, María Francisca De Lucia, Adrían Darío Gilli, Javier Ramon Bogado, Noelia Bonnecarrére, Victoria German, Silvia Marcelino, Francismar Ledesma, Fernando Reznikov, Sebastian Ploper, Leonardo Daniel Welin, Bjorn Castagnaro, Atilio Pedro Phakopsora pachyrhizi Polimorfismo de Longitud de Fragmentos Amplificados Marcadores Genéticos Soja Variación Genética Amplified Fragment Length Polymorphism Genetic Markers Soybeans Genetic Variation AFLP Marcadores Moleculares Background: Asian soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. & Syd., is one of the main diseases affecting soybean and has been reported as one of the most economically important fungal pathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered when developing resistance breeding strategies. We aimed to analyze the genetic diversity of P. pachyrhizi combining simple sampling with a powerful and reproducible molecular technique. Results: We employed Amplified Fragment Length Polymorphism (AFLP) technique for the amplification of P. pachyrhizi DNA extracted from naturally SBR-infected plants from 23 production fields. From a total of 1919 markers obtained, 77% were polymorphic. The high percentage of polymorphism and the Nei's genetic diversity coefficient (0.22) indicated high pathogen diversity. Analysis of molecular variance showed higher genetic variation within countries than among them. Temporal analysis showed a higher genetic variation within a year than between years. Cluster, phylogenetic and principal co-ordinate analysis showed that samples group by year of collection and then by country sampled. Conclusions: The study proposed combining a simple collection of urediniospore with a subsequent analysis by AFLP was useful to examine the molecular polymorphism of samples of P. pachyrhizi collected and might have a significant contribution to the knowledge of its genetic diversity. Also, AFLP analysis is an important and potent molecular tool for the study of genetic diversity and could be useful to carry out wider genetic diversity studies. EEA Cerro Azul Fil: Rocha, Carla Maria Lourdes. Gobierno de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial Obispo Colombres; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino; Argentina Fil: Vellicce, Gabriel Ricardo. Gobierno de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial Obispo Colombres; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino; Argentina Fil: Garcia, Maria Gabriela. Gobierno de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial Obispo Colombres; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino; Argentina Fil: Pardo, Esteban Mariano. Gobierno de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial Obispo Colombres; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino; Argentina Fil: Racedo, Josefina. Gobierno de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial Obispo Colombres; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino; Argentina Fil: Perera, María Francisca. Gobierno de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial Obispo Colombres; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino; Argentina Fil: De Lucia, Adrían Darío. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cerro Azul; Argentina Fil: Gilli, Javier Ramon. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Bogado, Noelia. Instituto Paraguayo de Tecnología Agraria. Centro de Investigación Capitán Miranda; Paraguay Fil: Bonnecarrère, Victoria. Instituto Nacional de Investigación Agropecuaria. Estación Experimental Wilson Ferreira Aldunate. Unidad de Biotecnología; Uruguay Fil: German, Silvia. Instituto Nacional de Investigación Agropecuaria. Estación Experimental Wilson Ferreira Aldunate. Unidad de Biotecnología; Uruguay Fil: Marcelino, Francismar. Empresa Brasilera de Pesquisa Agropecuaria (Embrapa). Soja; Brasil Fil: Ledesma, Fernando. Gobierno de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial Obispo Colombres; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino; Argentina Fil: Reznikov, Sebastian. Gobierno de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial Obispo Colombres; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino; Argentina Fil: Ploper, Leonardo Daniel. Gobierno de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial Obispo Colombres; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino; Argentina Fil: Welin, Bjorn. Gobierno de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial Obispo Colombres; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino; Argentina Fil: Castagnaro, Atilio Pedro. Gobierno de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial Obispo Colombres; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino; Argentina 2018-07-04T13:42:05Z 2018-07-04T13:42:05Z 2015-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0717345815001268 http://hdl.handle.net/20.500.12123/2721 0717-3458 https://doi.org/10.1016/j.ejbt.2015.06.007 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Electronic Journal of Biotechnology 18 (6) : 439-444 (November 2015) |
| spellingShingle | Phakopsora pachyrhizi Polimorfismo de Longitud de Fragmentos Amplificados Marcadores Genéticos Soja Variación Genética Amplified Fragment Length Polymorphism Genetic Markers Soybeans Genetic Variation AFLP Marcadores Moleculares Rocha, Carla Maria Lourdes Vellicce, Gabriel Ricardo Garcia, Maria Gabriela Pardo, Esteban Mariano Racedo, Josefina Perera, María Francisca De Lucia, Adrían Darío Gilli, Javier Ramon Bogado, Noelia Bonnecarrére, Victoria German, Silvia Marcelino, Francismar Ledesma, Fernando Reznikov, Sebastian Ploper, Leonardo Daniel Welin, Bjorn Castagnaro, Atilio Pedro Use of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi |
| title | Use of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi |
| title_full | Use of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi |
| title_fullStr | Use of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi |
| title_full_unstemmed | Use of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi |
| title_short | Use of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi |
| title_sort | use of aflp markers to estimate molecular diversity of phakopsora pachyrhizi |
| topic | Phakopsora pachyrhizi Polimorfismo de Longitud de Fragmentos Amplificados Marcadores Genéticos Soja Variación Genética Amplified Fragment Length Polymorphism Genetic Markers Soybeans Genetic Variation AFLP Marcadores Moleculares |
| url | https://www.sciencedirect.com/science/article/pii/S0717345815001268 http://hdl.handle.net/20.500.12123/2721 https://doi.org/10.1016/j.ejbt.2015.06.007 |
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