Panax ginseng extract reduces Staphylococcus aureus internalization into bovine mammary epithelial cells but does not affect macrophages phagocytic activity
Panax ginseng extract (PGe) has been shown to possess immunomodulatory effects in healthy dairy cows at drying off and to trigger an adequate immune response to protect from an experimental intramammary infection (IMI) with Staphylococcus aureus in a murine model. S. aureus is one of the major patho...
| Autores principales: | , , , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
|
| Materias: | |
| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S088240101731029X http://hdl.handle.net/20.500.12123/2719 https://doi.org/10.1016/j.micpath.2018.06.010 |
| _version_ | 1855483107533127680 |
|---|---|
| author | Beccaria, Camila Silvestrini, Paula Renna, María Sol Ortega, Hugo Hector Calvinho, Luis Fernando Dallard, Bibiana Elisabet Baravalle, Celina |
| author_browse | Baravalle, Celina Beccaria, Camila Calvinho, Luis Fernando Dallard, Bibiana Elisabet Ortega, Hugo Hector Renna, María Sol Silvestrini, Paula |
| author_facet | Beccaria, Camila Silvestrini, Paula Renna, María Sol Ortega, Hugo Hector Calvinho, Luis Fernando Dallard, Bibiana Elisabet Baravalle, Celina |
| author_sort | Beccaria, Camila |
| collection | INTA Digital |
| description | Panax ginseng extract (PGe) has been shown to possess immunomodulatory effects in healthy dairy cows at drying off and to trigger an adequate immune response to protect from an experimental intramammary infection (IMI) with Staphylococcus aureus in a murine model. S. aureus is one of the major pathogens isolated from bovine IMI; being capable to invade and survive within mammary epithelial cells. However, the precise mechanism by which PGe interacts with bovine mammary epithelial cells (MAC-T) and bovine macrophages in the course of a S. aureus infection remains unclear. We evaluated the effect of PGe on MAC-T cytokine response and on the internalization of S. aureus into MAC-T. In addition, we evaluated the effect of PGe on the phagocytic activity of macrophages isolated from bovine mammary secretions. Results shown that MAC-T cells TLR4 and NF-κB mRNA expression was not affected by PGe at all evaluated times. IL-6 mRNA expression and protein level and IL-4 protein level were significantly induced in MAC-T treated with 3 mg/ml of PGe. PGe at 3 mg/ml reduced significantly the internalization of two S. aureus strains in MAC-T. In addition, PGe did not affect the percentage of phagocytosis and the NO and ROS production of macrophages co-cultured with two strains of S. aureus. These results, obtained in in vitro models together with those obtained in in vivo previous studies carried out in bovines and mice can contribute to improve the understanding of the effects of PGe following inoculation in bovine mammary glands. |
| format | Artículo |
| id | INTA2719 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA27192018-07-04T12:20:11Z Panax ginseng extract reduces Staphylococcus aureus internalization into bovine mammary epithelial cells but does not affect macrophages phagocytic activity Beccaria, Camila Silvestrini, Paula Renna, María Sol Ortega, Hugo Hector Calvinho, Luis Fernando Dallard, Bibiana Elisabet Baravalle, Celina Staphylococcus aureus Panax pseudoginseng Extractos Control de Enfermedades Mastítis Bovina Citoquinas Extracts Disease Control Bovine Mastitis Cytokines Ginseng Panax ginseng Células Epiteliales Mamarias Panax ginseng extract (PGe) has been shown to possess immunomodulatory effects in healthy dairy cows at drying off and to trigger an adequate immune response to protect from an experimental intramammary infection (IMI) with Staphylococcus aureus in a murine model. S. aureus is one of the major pathogens isolated from bovine IMI; being capable to invade and survive within mammary epithelial cells. However, the precise mechanism by which PGe interacts with bovine mammary epithelial cells (MAC-T) and bovine macrophages in the course of a S. aureus infection remains unclear. We evaluated the effect of PGe on MAC-T cytokine response and on the internalization of S. aureus into MAC-T. In addition, we evaluated the effect of PGe on the phagocytic activity of macrophages isolated from bovine mammary secretions. Results shown that MAC-T cells TLR4 and NF-κB mRNA expression was not affected by PGe at all evaluated times. IL-6 mRNA expression and protein level and IL-4 protein level were significantly induced in MAC-T treated with 3 mg/ml of PGe. PGe at 3 mg/ml reduced significantly the internalization of two S. aureus strains in MAC-T. In addition, PGe did not affect the percentage of phagocytosis and the NO and ROS production of macrophages co-cultured with two strains of S. aureus. These results, obtained in in vitro models together with those obtained in in vivo previous studies carried out in bovines and mice can contribute to improve the understanding of the effects of PGe following inoculation in bovine mammary glands. EEA Rafaela Fil: Beccaría, Camila. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Silvestrini, Paula. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Renna, María Sol. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Ortega, Hugo Hector. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Dallard, Bibiana Elisabet. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Baravalle, Celina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina 2018-07-04T12:18:34Z 2018-07-04T12:18:34Z 2018-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S088240101731029X http://hdl.handle.net/20.500.12123/2719 0882-4010 https://doi.org/10.1016/j.micpath.2018.06.010 eng info:eu-repo/semantics/restrictedAccess application/pdf Microbial Pathogenesis 122 : 63-72 (September 2018) |
| spellingShingle | Staphylococcus aureus Panax pseudoginseng Extractos Control de Enfermedades Mastítis Bovina Citoquinas Extracts Disease Control Bovine Mastitis Cytokines Ginseng Panax ginseng Células Epiteliales Mamarias Beccaria, Camila Silvestrini, Paula Renna, María Sol Ortega, Hugo Hector Calvinho, Luis Fernando Dallard, Bibiana Elisabet Baravalle, Celina Panax ginseng extract reduces Staphylococcus aureus internalization into bovine mammary epithelial cells but does not affect macrophages phagocytic activity |
| title | Panax ginseng extract reduces Staphylococcus aureus internalization into bovine mammary epithelial cells but does not affect macrophages phagocytic activity |
| title_full | Panax ginseng extract reduces Staphylococcus aureus internalization into bovine mammary epithelial cells but does not affect macrophages phagocytic activity |
| title_fullStr | Panax ginseng extract reduces Staphylococcus aureus internalization into bovine mammary epithelial cells but does not affect macrophages phagocytic activity |
| title_full_unstemmed | Panax ginseng extract reduces Staphylococcus aureus internalization into bovine mammary epithelial cells but does not affect macrophages phagocytic activity |
| title_short | Panax ginseng extract reduces Staphylococcus aureus internalization into bovine mammary epithelial cells but does not affect macrophages phagocytic activity |
| title_sort | panax ginseng extract reduces staphylococcus aureus internalization into bovine mammary epithelial cells but does not affect macrophages phagocytic activity |
| topic | Staphylococcus aureus Panax pseudoginseng Extractos Control de Enfermedades Mastítis Bovina Citoquinas Extracts Disease Control Bovine Mastitis Cytokines Ginseng Panax ginseng Células Epiteliales Mamarias |
| url | https://www.sciencedirect.com/science/article/pii/S088240101731029X http://hdl.handle.net/20.500.12123/2719 https://doi.org/10.1016/j.micpath.2018.06.010 |
| work_keys_str_mv | AT beccariacamila panaxginsengextractreducesstaphylococcusaureusinternalizationintobovinemammaryepithelialcellsbutdoesnotaffectmacrophagesphagocyticactivity AT silvestrinipaula panaxginsengextractreducesstaphylococcusaureusinternalizationintobovinemammaryepithelialcellsbutdoesnotaffectmacrophagesphagocyticactivity AT rennamariasol panaxginsengextractreducesstaphylococcusaureusinternalizationintobovinemammaryepithelialcellsbutdoesnotaffectmacrophagesphagocyticactivity AT ortegahugohector panaxginsengextractreducesstaphylococcusaureusinternalizationintobovinemammaryepithelialcellsbutdoesnotaffectmacrophagesphagocyticactivity AT calvinholuisfernando panaxginsengextractreducesstaphylococcusaureusinternalizationintobovinemammaryepithelialcellsbutdoesnotaffectmacrophagesphagocyticactivity AT dallardbibianaelisabet panaxginsengextractreducesstaphylococcusaureusinternalizationintobovinemammaryepithelialcellsbutdoesnotaffectmacrophagesphagocyticactivity AT baravallecelina panaxginsengextractreducesstaphylococcusaureusinternalizationintobovinemammaryepithelialcellsbutdoesnotaffectmacrophagesphagocyticactivity |