A cost-effective and customizable automated irrigation system for precise high-throughput phenotyping in drought stress studies
The development of high-yielding crops with drought tolerance is necessary to increase food, feed, fiber and fuel production. Methods that create similar environmental conditions for a large number of genotypes are essential to investigate plant responses to drought in gene discovery studies. Modern...
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| Format: | Artículo |
| Language: | Inglés |
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2018
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| Online Access: | http://hdl.handle.net/20.500.12123/2697 http://journals.plos.org/plosone/article/authors?id=10.1371/journal.pone.0198546 https://doi.org/10.1371/journal.pone.0198546 |
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| author | Ortiz, Diego Litvin, Alexander G. Salas Fernandez, María G. |
| author_browse | Litvin, Alexander G. Ortiz, Diego Salas Fernandez, María G. |
| author_facet | Ortiz, Diego Litvin, Alexander G. Salas Fernandez, María G. |
| author_sort | Ortiz, Diego |
| collection | INTA Digital |
| description | The development of high-yielding crops with drought tolerance is necessary to increase food, feed, fiber and fuel production. Methods that create similar environmental conditions for a large number of genotypes are essential to investigate plant responses to drought in gene discovery studies. Modern facilities that control water availability for each plant remain cost-prohibited to some sections of the research community. We present an alternative cost-effective automated irrigation system scalable for a high-throughput and controlled dry-down treatment of plants. This system was tested in sorghum using two experiments. First, four genotypes were subjected to ten days of dry-down to achieve three final Volumetric Water Content (VWC) levels: drought (0.10 and 0.20 m3 m-3) and control (0.30 m3 m-3). The final average VWC was 0.11, 0.22, and 0.31 m3 m-3, respectively, and significant differences in biomass accumulation were observed between control and drought treatments. Second, 42 diverse sorghum genotypes were subjected to a seven-day dry-down treatment for a final drought stress of 0.15 m3 m-3 VWC. The final average VWC was 0.17 m3 m-3, and plants presented significant differences in photosynthetic rate during the drought period. These results demonstrate that cost-effective automation systems can successfully control substrate water content for each plant, to accurately compare their phenotypic responses to drought, and be scaled up for high-throughput phenotyping studies. |
| format | Artículo |
| id | INTA2697 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA26972024-05-20T10:55:59Z A cost-effective and customizable automated irrigation system for precise high-throughput phenotyping in drought stress studies Ortiz, Diego Litvin, Alexander G. Salas Fernandez, María G. Sorghum Sorgo Sequía Fenotipos Riego Automático Drought Phenotypes Automatic Irrigation The development of high-yielding crops with drought tolerance is necessary to increase food, feed, fiber and fuel production. Methods that create similar environmental conditions for a large number of genotypes are essential to investigate plant responses to drought in gene discovery studies. Modern facilities that control water availability for each plant remain cost-prohibited to some sections of the research community. We present an alternative cost-effective automated irrigation system scalable for a high-throughput and controlled dry-down treatment of plants. This system was tested in sorghum using two experiments. First, four genotypes were subjected to ten days of dry-down to achieve three final Volumetric Water Content (VWC) levels: drought (0.10 and 0.20 m3 m-3) and control (0.30 m3 m-3). The final average VWC was 0.11, 0.22, and 0.31 m3 m-3, respectively, and significant differences in biomass accumulation were observed between control and drought treatments. Second, 42 diverse sorghum genotypes were subjected to a seven-day dry-down treatment for a final drought stress of 0.15 m3 m-3 VWC. The final average VWC was 0.17 m3 m-3, and plants presented significant differences in photosynthetic rate during the drought period. These results demonstrate that cost-effective automation systems can successfully control substrate water content for each plant, to accurately compare their phenotypic responses to drought, and be scaled up for high-throughput phenotyping studies. EEA Manfredi Fil: Ortìz, Diego. Iowa State University. Departament of Agronomy; Estados Unidos Fil: Litvin, Alexander G. Iowa State University. Departament of Horticulture; Estados Unidos Fil: Salas Fernandez, Marìa G. Iowa State University. Departament of Horticulture; Estados Unidos 2018-06-29T13:31:37Z 2018-06-29T13:31:37Z 2018-06-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/2697 http://journals.plos.org/plosone/article/authors?id=10.1371/journal.pone.0198546 1932-6203 https://doi.org/10.1371/journal.pone.0198546 eng info:eu-repograntAgreement/INTA/PNCYO/1127043/AR./Desarrollo de germoplasma y cultivares comerciales de cereales de verano. info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf PLoS ONE 13 (6) : e0198546 (2018) |
| spellingShingle | Sorghum Sorgo Sequía Fenotipos Riego Automático Drought Phenotypes Automatic Irrigation Ortiz, Diego Litvin, Alexander G. Salas Fernandez, María G. A cost-effective and customizable automated irrigation system for precise high-throughput phenotyping in drought stress studies |
| title | A cost-effective and customizable automated irrigation system for precise high-throughput phenotyping in drought stress studies |
| title_full | A cost-effective and customizable automated irrigation system for precise high-throughput phenotyping in drought stress studies |
| title_fullStr | A cost-effective and customizable automated irrigation system for precise high-throughput phenotyping in drought stress studies |
| title_full_unstemmed | A cost-effective and customizable automated irrigation system for precise high-throughput phenotyping in drought stress studies |
| title_short | A cost-effective and customizable automated irrigation system for precise high-throughput phenotyping in drought stress studies |
| title_sort | cost effective and customizable automated irrigation system for precise high throughput phenotyping in drought stress studies |
| topic | Sorghum Sorgo Sequía Fenotipos Riego Automático Drought Phenotypes Automatic Irrigation |
| url | http://hdl.handle.net/20.500.12123/2697 http://journals.plos.org/plosone/article/authors?id=10.1371/journal.pone.0198546 https://doi.org/10.1371/journal.pone.0198546 |
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